|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||123 Antibodies||135 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies||23 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||66 Antibodies|
|Immunochromatography (IC)||3 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||28 Antibodies|
|Immunofluorescence (IF)||53 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||220 Antibodies|
|Immunohistochemistry (IHC)||165 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||94 Antibodies|
|Immunoprecipitation (IP)||21 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies|
|Western Blotting (WB)||393 Antibodies|
|Cytometry by Time of Flight (CyTOF)||4 Antibodies|
|Antigen||Epidermal Growth Factor Receptor (EGFR) Antibodies|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This EGFR antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-EGFR AntibodyTarget Details EGFR Application Details Handling Images
|Specificity||This antibody recognizes Human Epidermal Growth Factor Receptor.|
Species reactivity (expected):Chicken, Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Protein G Chromatography.|
|Immunogen||Recombinant 20 amino acid residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.|
Target Details EGFRProduct Details anti-EGFR Antibody Application Details Handling Images back to top
|Alternative Name||EGFR / ERBB1 (EGFR Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
ELISA. Western Blot. Immunoprecipitation. Immunocytochemistry. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
|Buffer||0.15 M PBS, pH 7.2 without preservatives|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ImagesProduct Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top