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anti-Dog (Canine) EGFR antibody for Immunohistochemistry (Frozen Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pTyr1173
127
56
55
52
51
49
48
45
45
44
44
33
32
30
30
25
23
23
23
22
21
19
18
17
16
15
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
7
7
7
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
1700
661
590
20
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1264
392
36
25
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
74
57
45
35
25
25
23
22
21
21
21
21
21
21
20
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1102
500
380
239
232
204
175
141
86
60
54
53
25
11
9
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126779
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008866263 ABIN126776 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr1173 Log in to see 9H2

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 135 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 188 Antibodies
Immunohistochemistry (IHC) 163 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 96 Antibodies
Immunoprecipitation (IP) 36 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 444 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1173
(127), (56), (55), (52), (51), (49), (48), (45), (45), (44), (44), (33), (32), (30), (30), (25), (23), (23), (23), (22), (21), (19), (18), (17), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(1700), (661), (590), (20), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1264), (392), (36), (25), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(74), (57), (45), (35), (25), (25), (23), (22), (21), (21), (21), (21), (21), (21), (20), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1102), (500), (380), (239), (232), (204), (175), (141), (86), (60), (54), (53), (25), (11), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity Clone 9H2 specifically interacts with the 1170 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR. Antibody AM00042PU-N does not crossreact with the highly homologous pTyr1248 of acticated ErbB2.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Purified from Serum-Free Cell Culture Supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHEpitope: phospho-Y1197 N A E pY L R V
Clone 9H2
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Usecelllysate delivered with this product as positive control: see protocol below! Immunoprecipitation (use at 1-10 μg per 10^6 vanadate-treated A431 cells). Immunocytochemistry (use at 1-10 μg/mL). Immunohistochemistry on Frozen and Paraffin Embedded Material. ELISA (use at 0.05 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control cell lysate provided: HepG2 Pervanadate Treated-recommended for ImmunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withpervanadate. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoid repeated freezing and thawing. Application: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysate contains SDS and is not recommended for applications withnative proteins such as immunoprecipitation !
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Reconstitution Restore with 1.0 mL H2O for 15 min at RT
Buffer PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles!
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1173) (ABIN126779) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody