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anti-Human EGFR antibody for Cell-ELISA

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Reactivity
Human
2147
741
607
21
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1374
686
60
44
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
93
73
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS)
1312
565
504
442
414
243
232
180
86
74
67
55
25
11
11
9
8
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN1381744
$ 358.05
Plus shipping costs $45.00

Top referenced anti-EGFR antibody for Cell-ELISA

Similar anti-EGFR Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 557 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 510 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 25 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 189 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2147), (741), (607), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1374), (686), (60), (44), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (73), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Cell-ELISA (cELISA), Flow Cytometry (FACS)
(1312), (565), (504), (442), (414), (243), (232), (180), (86), (74), (67), (55), (25), (11), (11), (9), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1381744) Images
Specificity Anti-human Receptor tyrosine-protein kinase ErbB-1 (EGFR)
Characteristics Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
Purification Protein G
Immunogen genetic immunisation with cDNA encoding human ErbB-1
Clone DP-4A1
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN1381744) Images back to top
Antigen
Alternative Name ErbB1 (EGFR Antibody Abstract)
Background Epidermal growth factor receptor (EGFR, ErbB-1) is a 170-kDa transmembrane glycoprotein encoded by the HER1 proto-oncogene. It belongs to the EGFR family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her3 (ErbB-3) and Her4 (ErbB-4). ErbB-1 is expressed on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Growth factor binding to the extracellular domain of ErbB-1 induces formation of homo- and heterodimers with another member of the ErbB receptor family. The EGFR signaling pathway is one of the most important pathways that regulate growth, survival, proliferation and differentiation in mammalian cells, while over-expression of ErbB-1 have been associated with a number of cancers, including lung cancer and glioblastoma.
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN1381744) Images back to top
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: EGFR

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN1381744) Images back to top
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C

References for anti-EGFR Antibody (ABIN1381744)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Normanno, Bianco, Strizzi, Mancino, Maiello, De Luca, Caponigro, Salomon: "The ErbB receptors and their ligands in cancer: an overview." in: Current drug targets, Vol. 6, Issue 3, pp. 243-57, 2005

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1381744) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN1381744) FACS analysis of BOSC23 cells using DP-4A1. BOSC23 cells were transiently transfected...
 image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN1381744) CGE analysis of purified DP-4A1 monoclonal antibody. Lane 1: molecular weight marker,...