anti-Human EGFR antibody for Cell-ELISA

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Reactivity
Human
2440
850
684
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1480
858
81
44
9
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
104
82
73
65
56
54
54
32
32
28
28
28
28
28
28
28
26
25
24
24
21
21
21
21
21
21
20
18
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS)
1469
677
615
556
502
272
259
229
124
84
66
56
40
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1381744
$ 423.78
Plus shipping costs $45.00

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2440), (850), (684), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1480), (858), (81), (44), (9), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(104), (82), (73), (65), (56), (54), (54), (32), (32), (28), (28), (28), (28), (28), (28), (28), (26), (25), (24), (24), (21), (21), (21), (21), (21), (21), (20), (18), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cell-ELISA (cELISA), Flow Cytometry (FACS)
(1469), (677), (615), (556), (502), (272), (259), (229), (124), (84), (66), (56), (40), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN1381744) Images
Specificity Anti-human Receptor tyrosine-protein kinase ErbB-1 (EGFR)
Characteristics Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
Purification Protein G
Immunogen genetic immunisation with cDNA encoding human ErbB-1
Clone DP-4A1
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN1381744) Images back to top
Antigen
Alternative Name ErbB1 (EGFR Antibody Abstract)
Background Epidermal growth factor receptor (EGFR, ErbB-1) is a 170-kDa transmembrane glycoprotein encoded by the HER1 proto-oncogene. It belongs to the EGFR family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her3 (ErbB-3) and Her4 (ErbB-4). ErbB-1 is expressed on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). Growth factor binding to the extracellular domain of ErbB-1 induces formation of homo- and heterodimers with another member of the ErbB receptor family. The EGFR signaling pathway is one of the most important pathways that regulate growth, survival, proliferation and differentiation in mammalian cells, while over-expression of ErbB-1 have been associated with a number of cancers, including lung cancer and glioblastoma.
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN1381744) Images back to top
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: EGFR

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN1381744) Images back to top
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C

References for anti-EGFR antibody (ABIN1381744)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Normanno, Bianco, Strizzi, Mancino, Maiello, De Luca, Caponigro, Salomon: "The ErbB receptors and their ligands in cancer: an overview." in: Current drug targets, Vol. 6, Issue 3, pp. 243-57, 2005

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN1381744) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) antibody (ABIN1381744) FACS analysis of BOSC23 cells using DP-4A1. BOSC23 cells were transiently transfected...
 image for anti-Epidermal Growth Factor Receptor (EGFR) antibody (ABIN1381744) CGE analysis of purified DP-4A1 monoclonal antibody. Lane 1: molecular weight marker,...