anti-Human EGFR antibody for Dot Blot

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pTyr1016
148
122
62
59
57
53
52
51
48
48
48
43
38
36
33
30
29
27
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
16
15
15
15
15
15
15
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2334
850
699
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Rabbit
1434
798
67
44
9
3
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Dot Blot (DB), Western Blotting (WB)
1362
633
572
529
442
272
245
230
111
83
67
56
35
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN389891
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
29.194513 ABIN389792 DB WB Rabbit Ig pTyr1172 Log in to see Polyclonal 4
29.194513 ABIN389764 DB Rabbit Ig pTyr1125 Log in to see Polyclonal 4
29.194513 ABIN1881284 DB WB Rabbit pTyr998 Log in to see Polyclonal 5
29.194513 ABIN1881286 DB WB Rabbit pSer768 Log in to see Polyclonal 5
29.194513 ABIN1881283 DB WB Rabbit pTyr1069 Log in to see Polyclonal 5
29.194513 ABIN1881285 DB WB Rabbit pTyr1092 Log in to see Polyclonal 5
29.194513 ABIN3030843 DB ELISA WB Rabbit Ig Fraction pTyr1016 Log in to see Polyclonal
29.194513 ABIN3030846 DB ELISA WB Rabbit Ig Fraction pTyr998 Log in to see Polyclonal
29.194513 ABIN3030842 DB ELISA WB Rabbit Ig Fraction pSer768 Log in to see Polyclonal
29.194513 ABIN3030841 DB ELISA WB Rabbit Ig Fraction pSer1070 Log in to see Polyclonal
29.194513 ABIN3030844 DB ELISA WB Rabbit Ig Fraction pTyr1092 Log in to see Polyclonal
29.194513 ABIN3030845 DB ELISA WB Rabbit Ig Fraction pTyr1172 Log in to see Polyclonal
29.194513 ABIN3030840 DB ELISA WB Rabbit Ig Fraction pTyr1069 Log in to see Polyclonal
29.194513 ABIN3030836 DB ELISA Rabbit Ig Fraction pTyr1125 Log in to see Polyclonal
1 ABIN1944845 DB WB Rabbit Ig pSer1070 Log in to see Polyclonal 5
1 ABIN1953146 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG pTyr1125 Log in to see Polyclonal
1 ABIN1955273 DB ELISA WB Biotin Rabbit IgG pTyr1125 Log in to see Polyclonal
1 ABIN1956453 DB ELISA WB FITC Rabbit IgG pTyr1125 Log in to see Polyclonal
1 ABIN1954018 DB ELISA WB APC Rabbit IgG pTyr1125 Log in to see Polyclonal
1 ABIN1958791 DB ELISA WB PE Rabbit IgG pTyr1125 Log in to see Polyclonal

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Cytometry by Time of Flight (CyTOF) 35 Antibodies
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General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1016
(148), (122), (62), (59), (57), (53), (52), (51), (48), (48), (48), (43), (38), (36), (33), (30), (29), (27), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2334), (850), (699), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1434), (798), (67), (44), (9), (3), (2), (1), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Dot Blot (DB), Western Blotting (WB)
(1362), (633), (572), (529), (442), (272), (245), (230), (111), (83), (67), (56), (35), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN389891) Images
Specificity This Phospho-EGFR-pY1016 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Clone RB11166
Isotype Ig

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN389891) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. It is involved in the control of cell growth and differentiation.
Synonyms: Epidermal growth factor receptor [Precursor], Receptor tyrosine-protein kinase ErbB-1, EGFR, ERBB1
Molecular Weight 134277 DA
Gene ID 1956
UniProt P00533
Research Area Cell Cycle, Phospho-specific antibodies, Cell Structure
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling ProductDetails: References for anti-EGFR antibody (ABIN389891) Images back to top
Application Notes WB = 1:8000, DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details ProductDetails: References for anti-EGFR antibody (ABIN389891) Images back to top
Format Liquid
Concentration 0.3 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-EGFR antibody (ABIN389891)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Aifa, Aydin, Nordvall, Lundström, Svensson, Hermanson: "A basic peptide within the juxtamembrane region is required for EGF receptor dimerization." in: Experimental cell research, Vol. 302, Issue 1, pp. 108-14, 2004

Kuribayashi, Kataoka, Kurabayashi, Miura: "Evidence that basal activity, but not transactivation, of the epidermal growth factor receptor tyrosine kinase is required for insulin-like growth factor I-induced activation of extracellular signal-regulated kinase in oral carcinoma cells." in: Endocrinology, Vol. 145, Issue 11, pp. 4976-84, 2004

Kapoor, Zhan, Johnson, ORourke: "Distinct domains in the SHP-2 phosphatase differentially regulate epidermal growth factor receptor/NF-kappaB activation through Gab1 in glioblastoma cells." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 823-36, 2003

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN389891) back to top
Supplier Images
Dot Blot (DB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1016) (ABIN389891) Dot blot analysis of anti-EGFR B1 Phospho-specific Pab (ABIN389891) on nitrocellulose...