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anti-Human EGFR antibody for Dot Blot

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pTyr1016
146
138
57
57
53
51
48
48
46
45
44
44
35
33
32
30
30
25
24
23
23
23
23
22
22
19
18
17
16
14
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2147
741
607
21
14
14
12
10
7
7
4
2
1
1
1
Host
Rabbit
1373
687
60
44
4
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
93
73
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Dot Blot (DB), Western Blotting (WB)
1311
565
505
442
414
243
232
180
85
74
67
55
25
11
11
9
8
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN389891
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.6397257 ABIN389764 DB Rabbit Ig pTyr1125 Log in to see Polyclonal 4
0.6397257 ABIN389792 DB WB Rabbit Ig pTyr1172 Log in to see Polyclonal 4
0.6397257 ABIN1881286 DB WB Rabbit pSer768 Log in to see Polyclonal 5
0.6397257 ABIN1881284 DB WB Rabbit pTyr998 Log in to see Polyclonal 5
0.6397257 ABIN1881283 DB WB Rabbit pTyr1069 Log in to see Polyclonal 5
0.6397257 ABIN1881285 DB WB Rabbit pTyr1092 Log in to see Polyclonal 5
0.6397257 ABIN2160828 DB Rabbit Ig Fraction pThr1125 Log in to see Polyclonal
0.6397257 ABIN2158648 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.6397257 ABIN2158649 DB ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.0008925438 ABIN1944845 DB WB Rabbit Ig pSer1070 Log in to see Polyclonal 5
0.0008925438 ABIN1953000 DB IHC ELISA Alkaline Phosphatase (AP) Rabbit IgG Ser768, pSer768 Log in to see Polyclonal
0.0008925438 ABIN1953141 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Tyr1016, pTyr1016 Log in to see Polyclonal
0.0008925438 ABIN1953146 DB ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Tyr1125, pTyr1125 Log in to see Polyclonal
0.0008925438 ABIN1953150 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Tyr1172, pTyr1172 Log in to see Polyclonal
0.0008925438 ABIN1953872 DB IHC ELISA APC Rabbit IgG Ser768, pSer768 Log in to see Polyclonal
0.0008925438 ABIN1954013 DB ELISA APC Rabbit IgG Tyr1016, pTyr1016 Log in to see Polyclonal
0.0008925438 ABIN1954018 DB ELISA WB APC Rabbit IgG Tyr1125, pTyr1125 Log in to see Polyclonal
0.0008925438 ABIN1954022 DB ELISA APC Rabbit IgG Tyr1172, pTyr1172 Log in to see Polyclonal
0.0008925438 ABIN1956313 DB IHC ELISA FITC Rabbit IgG Ser768, pSer768 Log in to see Polyclonal
0.0008925438 ABIN1956448 DB ELISA FITC Rabbit IgG Tyr1016, pTyr1016 Log in to see Polyclonal

Top referenced anti-EGFR antibody for Dot Blot

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Application / Reactivity Human
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Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 557 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 510 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
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Immunochromatography (IC) 25 Antibodies
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Immunofluorescence (IF) 189 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 232 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1016
(146), (138), (57), (57), (53), (51), (48), (48), (46), (45), (44), (44), (35), (33), (32), (30), (30), (25), (24), (23), (23), (23), (23), (22), (22), (19), (18), (17), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2147), (741), (607), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Rabbit
(1373), (687), (60), (44), (4), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (73), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Dot Blot (DB), Western Blotting (WB)
(1311), (565), (505), (442), (414), (243), (232), (180), (85), (74), (67), (55), (25), (11), (11), (9), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN389891) Images
Specificity This Phospho-EGFR-pY1016 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR.
Clone RB11166
Isotype Ig

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN389891) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. It is involved in the control of cell growth and differentiation.
Synonyms: Epidermal growth factor receptor [Precursor], Receptor tyrosine-protein kinase ErbB-1, EGFR, ERBB1
Molecular Weight 134277 DA
Gene ID 1956
UniProt P00533
Research Area Cell Cycle, Phospho-specific antibodies, Cell Structure
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN389891) Images back to top
Application Notes WB = 1:8000, DB = 1:500
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN389891) Images back to top
Format Liquid
Concentration 0.3 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-EGFR Antibody (ABIN389891)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Aifa, Aydin, Nordvall, Lundström, Svensson, Hermanson: "A basic peptide within the juxtamembrane region is required for EGF receptor dimerization." in: Experimental cell research, Vol. 302, Issue 1, pp. 108-14, 2004

Kuribayashi, Kataoka, Kurabayashi, Miura: "Evidence that basal activity, but not transactivation, of the epidermal growth factor receptor tyrosine kinase is required for insulin-like growth factor I-induced activation of extracellular signal-regulated kinase in oral carcinoma cells." in: Endocrinology, Vol. 145, Issue 11, pp. 4976-84, 2004

Kapoor, Zhan, Johnson, ORourke: "Distinct domains in the SHP-2 phosphatase differentially regulate epidermal growth factor receptor/NF-kappaB activation through Gab1 in glioblastoma cells." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 823-36, 2003

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN389891) back to top
Supplier Images
Dot Blot (DB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1016) (ABIN389891) Dot blot analysis of anti-EGFR B1 Phospho-specific Pab (ABIN389891) on nitrocellulose...