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anti-Human EGFR antibody for ELISA

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
AA 693-893
146
138
57
57
53
51
49
48
46
45
44
44
35
33
32
30
30
25
24
23
23
23
23
22
22
19
18
17
16
14
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
8
8
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2147
741
607
21
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1374
686
60
44
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
93
73
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
1311
564
504
442
414
243
232
180
86
74
67
55
25
11
11
9
8
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN1724889
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32753587 ABIN1724890 FACS IHC ELISA WB Mouse IgG1 AA 693-893 Log in to see 7A6F12 2
0.32753587 ABIN1724825 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
0.32753587 ABIN1724824 FACS IHC ELISA WB Mouse IgG1 AA 693-893, Mutant Log in to see 5G9B5 2
0.32753587 ABIN515187 ELISA Mouse IgG2a kappa AA 26-125, partial Log in to see 4H2 1
0.32753587 ABIN1847548 ELISA WB Rabbit C-Term, pSer1026, pSer1028, pSer1027 Log in to see Polyclonal
0.0012091895 ABIN98862 IP ELISA WB Rabbit Log in to see Polyclonal
0.0012091895 ABIN1532007 ELISA WB Rabbit IgG pSer1026 Log in to see Polyclonal 1
0.0012091895 ABIN1532008 ELISA WB Rabbit IgG pSer1071 Log in to see Polyclonal 1
0.0012091895 ABIN1533044 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012091895 ABIN1533045 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012091895 ABIN1532602 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012091895 ABIN1532852 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012091895 ABIN1533046 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012091895 ABIN284399 ICC IP ELISA WB Mouse IgG1 kappa pTyr1173 Log in to see 9H2
0.0012091895 ABIN967602 IHC (f) ELISA WB Mouse IgG1, kappa pTyr845 Log in to see 12A3 7
0.0012091895 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
0.0012091895 ABIN1742583 IHC (fro) IHC (p) IP ELISA Mouse IgG1 AA 985-996 Log in to see 1
0.0012091895 ABIN1584389 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
0.0012091895 ABIN934068 ELISA WB Mouse IgG2b kappa AA 424-605 Log in to see AT6E3 1
0.0012091895 ABIN1450404 ELISA WB Rabbit IgG pSer695, Internal Region Log in to see Polyclonal 1

Top referenced anti-EGFR antibody for ELISA

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ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 510 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
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Immunocytochemistry (ICC) 74 Antibodies
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Immunofluorescence (IF) 189 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 232 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 693-893
(146), (138), (57), (57), (53), (51), (49), (48), (46), (45), (44), (44), (35), (33), (32), (30), (30), (25), (24), (23), (23), (23), (23), (22), (22), (19), (18), (17), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2147), (741), (607), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1374), (686), (60), (44), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (73), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(1311), (564), (504), (442), (414), (243), (232), (180), (86), (74), (67), (55), (25), (11), (11), (9), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1724889) Images
Purification Purified
Immunogen Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.
Clone 5E10D3
Isotype IgG2b

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN1724889) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. ,
Molecular Weight 175 kDa
Gene ID 1956
HGNC 1956
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN1724889) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN1724889) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-EGFR Antibody (ABIN1724889)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Derer, Bauer, Lohse, Scheel, Berger, Kellner, Peipp, Valerius: "Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 11, pp. 5230-9, 2012

Minder, Bayha, Becker-Pauly, Sterchi: "Meprin? transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35201-11, 2012

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1724889) back to top
Supplier Images
ELISA image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 693-893) (ABIN1724889) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 693-893) (ABIN1724889) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody (Image 2)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 693-893) (ABIN1724889) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody (Image 3)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 693-893) (ABIN1724889) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody (Image 4)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 693-893) (ABIN1724889) anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody (Image 5)