anti-Human EGFR antibody for Immunofluorescence

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Epitope
AA 1020-1046
147
135
64
63
59
57
56
54
53
52
48
48
43
42
39
36
33
30
30
29
27
26
25
25
22
19
18
18
18
18
17
16
16
16
16
15
15
15
15
15
14
14
13
11
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2440
849
684
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1480
858
81
44
9
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
104
82
73
65
56
54
54
32
32
28
28
28
28
28
28
28
26
25
24
24
21
21
21
21
21
21
20
18
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1468
678
615
556
501
272
258
228
124
84
66
56
40
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN967696
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.687622 ABIN1532331 IF IHC ELISA WB Rabbit IgG AA 986-1035 Log in to see Polyclonal
11.687622 ABIN1531178 IF IHC ELISA WB Rabbit IgG AA 661-710, pThr693 Log in to see Polyclonal
11.687622 ABIN1531312 IF IHC ELISA WB Rabbit IgG AA 986-1035, pTyr1016 Log in to see Polyclonal
11.687622 ABIN1531177 IF ELISA WB Rabbit IgG AA 651-700, pThr678 Log in to see Polyclonal
11.687622 ABIN1532195 IF IHC ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal
11.687622 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 2
11.687622 ABIN4307205 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
11.687622 ABIN4307221 FACS IF IHC (p) Mouse IgG2a kappa Log in to see GFR450
11.687622 ABIN2854848 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
11.687622 ABIN1995313 FACS IF IF/ICC Mouse IgG1 AA 1-645 Log in to see 08
11.687622 ABIN515185 IF PLA WB Mouse AA 1-405, full length Log in to see Polyclonal 1
11.687622 ABIN2801210 IC IF IP IHC WB Rabbit C-Term, pTyr1016 Log in to see Polyclonal
11.687622 ABIN2706075 IC IF IHC WB Rabbit pSer693 Log in to see Polyclonal
11.687622 ABIN2706078 IC IF IHC WB Rabbit Center Log in to see Polyclonal
11.687622 ABIN2957555 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.687622 ABIN2704752 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.687622 ABIN3024084 FACS IF IHC Mouse IgG2a, kappa Log in to see GFR450
11.687622 ABIN4307285 ICC IF IHC IHC (p) IP WB Mouse IgG2b C-Term Log in to see 133
11.687622 ABIN1995321 IHC (fro) IF IHC (p) ELISA Rabbit IgG AA 1-645 Log in to see 043
11.687622 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 1020-1046
(147), (135), (64), (63), (59), (57), (56), (54), (53), (52), (48), (48), (43), (42), (39), (36), (33), (30), (30), (29), (27), (26), (25), (25), (22), (19), (18), (18), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2440), (849), (684), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1480), (858), (81), (44), (9), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(104), (82), (73), (65), (56), (54), (54), (32), (32), (28), (28), (28), (28), (28), (28), (28), (26), (25), (24), (24), (21), (21), (21), (21), (21), (21), (20), (18), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1468), (678), (615), (556), (501), (272), (258), (228), (124), (84), (66), (56), (40), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN967696) Images
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human EGF Receptor aa. 1020-1046
Clone 13-EGFR
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN967696) Images back to top
Antigen
Alternative Name EGF Receptor (EGFR Antibody Abstract)
Background Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cgamma. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos.
Molecular Weight 180 kDa
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN967696) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN967696) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-EGFR antibody (ABIN967696)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Deugnier, Faraldo, Janji, Rousselle, Thiery, Glukhova: "EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor properties." in: The Journal of cell biology, Vol. 159, Issue 3, pp. 453-63, 2002

Golubovskaya, Beviglia, Xu, Earp, Craven, Cance: "Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38978-87, 2002

Ford, Valle, Soroka, Merchant: "EGF receptor activation stimulates endogenous gastrin gene expression in canine G cells and human gastric cell cultures." in: The Journal of clinical investigation, Vol. 99, Issue 11, pp. 2762-71, 1997

Kizaka-Kondoh, Matsuda, Okayama: "CrkII signals from epidermal growth factor receptor to Ras." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 22, pp. 12177-82, 1996

Ward, Gough, Rashke, Wan, Tribbick, Wang: "Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor." in: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5603-9, 1996

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN967696) back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 1020-1046) antibody (ABIN967696) Western blot analysis of EGF Receptor on A431 lysate. Lane 1: 1:2500, lane 2: 1: 5000...
Immunofluorescence (IF) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 1020-1046) antibody (ABIN967696) Immunofluorescent staining of A431 cells with anti-EGF Receptor antibody.