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anti-Human EGFR antibody for Immunofluorescence

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
AA 1020-1046
146
140
58
57
53
51
50
48
46
45
44
44
35
33
32
30
30
25
24
23
23
23
23
22
22
19
18
17
16
14
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2159
748
611
23
14
14
13
10
8
7
4
2
1
1
1
1
1
Host
Mouse
1378
691
63
45
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
93
74
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1320
568
511
449
420
249
232
183
86
76
71
55
25
11
11
9
8
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN967696
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN1531178 IF IHC ELISA WB Rabbit IgG pThr693 Log in to see Polyclonal
0.32040194 ABIN1531177 IF ELISA WB Rabbit IgG pThr678 Log in to see Polyclonal
0.32040194 ABIN1531312 IF IHC ELISA WB Rabbit IgG pTyr1016 Log in to see Polyclonal
0.32040194 ABIN189322 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr1173 Log in to see E124
0.32040194 ABIN1532331 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.32040194 ABIN1532195 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.32040194 ABIN2854848 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.32040194 ABIN189316 ICC FACS IF IP WB Rabbit IgG C-Term Log in to see E235
0.32040194 ABIN515185 IF PLA WB Mouse AA 1-405, full length Log in to see Polyclonal 1
0.32040194 ABIN2706078 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.32040194 ABIN2706075 IC IF IHC WB Rabbit pSer693 Log in to see Polyclonal
0.32040194 ABIN2704752 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.32040194 ABIN189315 ICC IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see E114
0.32040194 ABIN1995313 FACS IF Mouse IgG1 AA 1-645 Log in to see 8
0.32040194 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.32040194 ABIN2164436 IF ELISA WB Rabbit IgG Thr678 Log in to see Polyclonal
0.32040194 ABIN2164439 IF IHC ELISA WB Rabbit IgG Tyr1016 Log in to see Polyclonal
0.32040194 ABIN2164440 IF IHC ELISA WB Rabbit IgG Thr693 Log in to see Polyclonal
0.32040194 ABIN2164458 IF IHC ELISA WB Rabbit IgG Thr693 Log in to see Polyclonal
0.32040194 ABIN1995321 IHC (fro) IF IHC (p) ELISA Rabbit IgG AA 1-645 Log in to see 43

Top referenced anti-EGFR antibody for Immunofluorescence

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 137 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 67 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 78 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 188 Antibodies
Immunohistochemistry (IHC) 186 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 134 Antibodies
Immunoprecipitation (IP) 40 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 485 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 1020-1046
(146), (140), (58), (57), (53), (51), (50), (48), (46), (45), (44), (44), (35), (33), (32), (30), (30), (25), (24), (23), (23), (23), (23), (22), (22), (19), (18), (17), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2159), (748), (611), (23), (14), (14), (13), (10), (8), (7), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(1378), (691), (63), (45), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (74), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1320), (568), (511), (449), (420), (249), (232), (183), (86), (76), (71), (55), (25), (11), (11), (9), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN967696) Images
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human EGF Receptor aa. 1020-1046
Clone 13-EGFR
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN967696) Images back to top
Antigen
Alternative Name EGF Receptor (EGFR Antibody Abstract)
Background Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cgamma. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos.
Molecular Weight 180 kDa
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN967696) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN967696) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-EGFR Antibody (ABIN967696)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Deugnier, Faraldo, Janji, Rousselle, Thiery, Glukhova: "EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor properties." in: The Journal of cell biology, Vol. 159, Issue 3, pp. 453-63, 2002

Golubovskaya, Beviglia, Xu, Earp, Craven, Cance: "Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38978-87, 2002

Ford, Valle, Soroka, Merchant: "EGF receptor activation stimulates endogenous gastrin gene expression in canine G cells and human gastric cell cultures." in: The Journal of clinical investigation, Vol. 99, Issue 11, pp. 2762-71, 1997

Kizaka-Kondoh, Matsuda, Okayama: "CrkII signals from epidermal growth factor receptor to Ras." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 22, pp. 12177-82, 1996

Ward, Gough, Rashke, Wan, Tribbick, Wang: "Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor." in: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5603-9, 1996

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN967696) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1020-1046) (ABIN967696) Western blot analysis of EGF Receptor on A431 lysate. Lane 1: 1:2500, lane 2: 1: 5000...
Immunofluorescence (IF) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1020-1046) (ABIN967696) Immunofluorescent staining of A431 cells with anti-EGF Receptor antibody.