anti-Human EGFR antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 975-1015
148
122
62
59
57
53
53
51
48
48
48
43
38
36
33
30
29
27
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
16
15
15
15
15
15
14
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2334
849
698
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Rabbit
1434
798
67
44
9
3
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1363
633
572
528
442
271
245
230
111
84
67
56
35
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN725720
$ 295.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN895017 IF (p) Alexa Fluor 350 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN895015 IF (p) Alexa Fluor 488 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN895012 IF (p) Alexa Fluor 555 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN2812702 IF (p) Alexa Fluor 594 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN895013 IF (p) Alexa Fluor 647 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN725723 IF (p) Cy3 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN725724 IF (p) Cy5 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN725725 IF (p) Cy5.5 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN725726 IF (p) Cy7 Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN725727 IF (p) FITC Rabbit IgG AA 975-1015 Log in to see Polyclonal
1 ABIN757977 IF (p) Cy5.5 Rabbit IgG pSer1070 Log in to see Polyclonal
1 ABIN878749 IF (p) Alexa Fluor 350 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN878745 IF (p) Alexa Fluor 488 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN878746 IF (p) Alexa Fluor 555 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN2812707 IF (p) Alexa Fluor 594 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN878747 IF (p) Alexa Fluor 647 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN684256 IF (p) Cy3 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN684257 IF (p) Cy5 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN684258 IF (p) Cy5.5 Rabbit IgG pTyr1138 Log in to see Polyclonal
1 ABIN684259 IF (p) Cy7 Rabbit IgG pTyr1138 Log in to see Polyclonal

Similar anti-EGFR Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 23 Antibodies 14 Antibodies 13 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies 4 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 1350 Antibodies 551 Antibodies 453 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies 1 Antibodies
Neutralization (Neut) 14 Antibodies
Luminex Assay (LMNX) 11 Antibodies
Intracellular Staining (ICS) 2 Antibodies 2 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 975-1015
(148), (122), (62), (59), (57), (53), (53), (51), (48), (48), (48), (43), (38), (36), (33), (30), (29), (27), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2334), (849), (698), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1434), (798), (67), (44), (9), (3), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(1363), (633), (572), (528), (442), (271), (245), (230), (111), (84), (67), (56), (35), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human EGFR
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

Subcellular location: Cytoplasm

Synonyms: ERBB, HER1, mENA, ERBB1, PIG61, NISBD2, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR

Gene ID 1956
UniProt P00533
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 975-1015) (ABIN725720) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-EGFR Poly...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 975-1015) (ABIN725720) Paraformaldehyde-fixed, paraffin embedded human brain glioma, Antigen retrieval by bo...