anti-Human EGFR antibody for Immunofluorescence (fixed cells)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 1-645
147
134
64
64
60
58
57
54
54
53
49
49
43
39
36
33
32
30
29
29
27
26
26
25
24
22
19
18
17
17
16
16
16
15
15
15
15
15
14
14
13
11
10
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2444
866
700
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1462
867
92
44
11
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
103
80
71
64
58
56
34
34
28
28
28
28
28
28
28
27
27
26
26
26
24
21
21
21
21
21
20
19
18
4
2
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
1452
701
615
556
511
272
259
230
127
84
66
56
45
23
19
14
12
11
7
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1995313
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1995319 ELISA ELISA (Detection) IF IF/ICC IP WB Rabbit IgG AA 1-645 Log in to see 021
1 ABIN1843432 IF/ICC WB Rabbit pThr678 Log in to see Polyclonal
1 ABIN1843440 IF/ICC IHC WB Rabbit pThr693 Log in to see Polyclonal
1 ABIN1843442 IF/ICC IHC WB Rabbit pTyr1016 Log in to see Polyclonal
1 ABIN1843444 IF/ICC IHC WB Rabbit Log in to see Polyclonal
1 ABIN1843446 IF/ICC IHC WB Rabbit Log in to see Polyclonal
1 ABIN1854890 FACS IF/ICC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2972433 IF/ICC IHC WB Rabbit Center Log in to see Polyclonal
1 ABIN2972435 IF/ICC IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2974353 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3071075 IF/ICC IHC IP WB Rabbit C-Term, pTyr1016 Log in to see Polyclonal
1 ABIN2971519 IF/ICC IHC WB Rabbit Center, pThr693 Log in to see Polyclonal
1 ABIN3038056 FACS IF/ICC IHC IP PE Mouse IgG2b Log in to see
1 ABIN3038053 FACS IF/ICC IHC IP Mouse IgG2b Log in to see
1 ABIN3038045 ELISA IF/ICC WB Rabbit Log in to see Polyclonal
1 ABIN3067264 IF/ICC IHC WB Mouse Log in to see
1 ABIN3067262 IF/ICC IP WB Mouse Log in to see
1 ABIN2927286 IF/ICC WB Rabbit Internal Region, pThr678 Log in to see Polyclonal
1 ABIN2927287 IF/ICC IHC WB Rabbit Internal Region, pThr693 Log in to see Polyclonal
1 ABIN2927960 ELISA IF/ICC IHC WB Rabbit AA 713-984 Log in to see Polyclonal

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 1-645
(147), (134), (64), (64), (60), (58), (57), (54), (54), (53), (49), (49), (43), (39), (36), (33), (32), (30), (29), (29), (27), (26), (26), (25), (24), (22), (19), (18), (17), (17), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (10), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2444), (866), (700), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1462), (867), (92), (44), (11), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(103), (80), (71), (64), (58), (56), (34), (34), (28), (28), (28), (28), (28), (28), (28), (27), (27), (26), (26), (26), (24), (21), (21), (21), (21), (21), (20), (19), (18), (4), (2), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(1452), (701), (615), (556), (511), (272), (259), (230), (127), (84), (66), (56), (45), (23), (19), (14), (12), (11), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity Human EGFR / HER1 / ErbB1
No cross-reactivity in ELISA with
Human ErbB2
Human ErbB3
Human ErbB4
No Cross-Reactivity Human
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1, ABIN2001845 NP_005219, Met1-Ser645,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human EGFR / HER1 / ErbB1 protein (ABIN2001845)
Clone 08
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is a type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR signaling plays a crucial role in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, initiates subsequent tyrosine autophosphorylation and various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling has been shown to exert actions on carcinogenesis and disease progression, and thus EGFR was proposed as a target for cancer therapy currently.
NCBI Accession NP_005219
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes ICC/IF: 5-20 μg/mL
FCM: 0.5-2 μg/Test
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 1-645) antibody (ABIN1995313) Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA...