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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Blocking Reagent (BR)||9 Antibodies|
|Cell-ELISA (cELISA)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||86 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||55 Antibodies|
|Flow Cytometry (FACS)||180 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||2 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||25 Antibodies|
|Immunocytochemistry (ICC)||66 Antibodies|
|Immunoelectron Microscopy (IEM)||2 Antibodies|
|Immunofluorescence (IF)||150 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||236 Antibodies|
|Antigen||Epidermal Growth Factor Receptor (EGFR) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Conjugate||This EGFR antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
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Product Details anti-EGFR AntibodyTarget Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN118950) Images
|Specificity||This antibody recognises the epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. This antibody binds to epitope B from EGFR (1, 2) and has an affinity of 6.7 x 10e-9 M.|
|Purification||Ion exchange chromatography|
|Immunogen||Extracellular domain of human Epidermal Growth Factor Receptor (EGFR) from head and neck carcinoma.|
Target Details EGFRProduct Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN118950) Images back to top
|Alternative Name||EGFR / ERBB1 (EGFR Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN118950) Images back to top
Immunoprecipitation. Western blotting. Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Recommended positive control: Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN118950) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-EGFR Antibody (ABIN118950)Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Modjtahedi, Dean: "The receptor for EGF and its ligands - expression, prognostic value and target for therapy in cancer (review)." in: International journal of oncology, Vol. 4, Issue 2, pp. 277-96, 2011 (PubMed).
Modjtahedi, Eccles, Box et al.: "Antitumor activity of combinations of antibodies directed against different epitopes on the extracellular domain of the human EGF receptor." in: Cell biophysics, Vol. 22, Issue 1-3, pp. 129-46, 1995 (PubMed).
ImagesProduct Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN118950) back to top