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anti-Human EGFR antibody for Immunohistochemistry (Frozen Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pTyr869
146
138
57
57
53
50
49
48
46
45
44
44
35
33
32
30
30
25
24
23
23
23
23
22
22
19
18
17
16
14
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2147
740
607
21
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1374
686
60
44
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
93
73
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1311
565
505
441
414
242
232
179
86
73
67
54
25
11
11
9
8
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126761
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088295416 ABIN118950 FACS IHC (fro) IP WB Rat IgG2a Extracellular Domain Log in to see ICR10 2
0.00088295416 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
0.00088295416 ABIN114788 IHC (fro) IHC (p) IP WB Mouse IgG1 Extracellular Domain Log in to see 111-6
0.00088295416 ABIN114789 IHC (fro) IHC (p) Mouse IgG1 Extracellular Domain Log in to see 111-6
0.00088295416 ABIN371623 IHC (fro) WB Rat IgG2a Log in to see SB41a 6
0.00088295416 ABIN780370 ICC IHC (fro) FACS IP Mouse IgG2b Extracellular Domain Log in to see EGFR1 3
0.00088295416 ABIN1742583 IHC (fro) IHC (p) IP ELISA Mouse IgG1 AA 985-996 Log in to see 1
0.00088295416 ABIN118951 IHC (fro) IP Rat IgG2a Log in to see ICR11 1
0.00088295416 ABIN120073 IHC (fro) IP Mouse IgG2b Log in to see ICR61 1
0.00088295416 ABIN1742582 IHC (fro) IP Mouse IgG2b Log in to see 1
0.00088295416 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
0.00088295416 ABIN111402 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Log in to see EGF-R2
0.00088295416 ABIN235069 IHC (fro) IHC (p) Biotin Mouse IgG1 Internal Region Log in to see F4
0.00088295416 ABIN118952 IHC (fro) Mouse IgG1 AA 985-996 Log in to see F4 3
0.00088295416 ABIN126776 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr1173 Log in to see 9H2
0.00088295416 ABIN492657 EIA FACS IHC (fro) IHC (p) Mouse IgG1 Extracellular Domain Log in to see EGF-R2
0.00088295416 ABIN211610 FACS IHC (fro) IP Rat IgG2a Extracellular Domain Log in to see ICR10
0.00088295416 ABIN249234 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 111-6
0.00088295416 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Extracellular Domain Log in to see ICR10
0.00088295416 ABIN1995321 IHC (fro) IF IHC (p) ELISA Rabbit IgG AA 1-645 Log in to see 43

Top referenced anti-EGFR antibody for Immunohistochemistry (Frozen Sections)

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 136 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 64 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 188 Antibodies
Immunohistochemistry (IHC) 182 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 129 Antibodies
Immunoprecipitation (IP) 36 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 482 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr869
(146), (138), (57), (57), (53), (50), (49), (48), (46), (45), (44), (44), (35), (33), (32), (30), (30), (25), (24), (23), (23), (23), (23), (22), (22), (19), (18), (17), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2147), (740), (607), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1374), (686), (60), (44), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(93), (73), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1311), (565), (505), (441), (414), (242), (232), (179), (86), (73), (67), (54), (25), (11), (11), (9), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031PU-N does not crossreact with the highly homologues pTyr 877 of activated erbB2.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 869AA Sequence: E K E pY H A E
Clone 12A3
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes ELISA: Use at 0.1 μg/mL (direct ELISA). Immunocytochemistry: Use at 1-10 μg/mL. Immunohistochemistry on Frozen and Paraffin Embedded Material. Immunoprecipitation: Use at 1-10 μg per 10^6 vanadate-treated A431 cells. Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer (cat#AS00002BU-N). Included Positive Control: Cell lysate delivered with this product (See Protocol below! ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Reconstitution Restore in 1 mL distilled water for 15 min at RT.
Buffer PBS containing 0.09 % Sodium Azide / PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at-80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3months. Avoid repeated freeze / thaw cycles!
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr869) (ABIN126761) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr869) antibody