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anti-Human EGFR antibody for Immunoprecipitation

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
Tyr992, pTyr992
127
56
55
52
51
49
48
45
45
44
44
33
32
30
30
25
23
23
23
22
21
19
18
17
16
16
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
7
7
7
6
6
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1700
662
590
21
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1264
392
36
25
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
74
57
45
35
25
25
23
22
21
21
21
21
21
21
20
2
2
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
1102
500
380
240
232
204
174
142
86
60
55
54
25
11
9
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN343716
$ 381.15
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.49796146 ABIN343717 FACS IP WB Mouse IgG1 Tyr1173, pTyr1173 Log in to see EM-13 5
0.49796146 ABIN2169503 ICC IP WB Mouse IgG1 Log in to see 6H11-2D11-G3
0.49796146 ABIN2169502 ICC IP WB Mouse IgG1 Log in to see 5E10-G10-E11
0.49796146 ABIN2169501 IP WB Mouse IgG1 Log in to see 1A11-G9-1E9
0.49796146 ABIN2169500 ICC IP IHC WB Mouse IgG1 Log in to see 3F12-1H7-A10
0.49796146 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0009004611 ABIN98862 IP ELISA WB Rabbit Log in to see Polyclonal
0.0009004611 ABIN452102 FACS IF IHC (p) IP WB Mouse IgG1 Log in to see H11 1
0.0009004611 ABIN126767 EIA IF IP WB Mouse IgG1 pSer1071 Log in to see 1H9 1
0.0009004611 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
0.0009004611 ABIN118950 FACS IHC (fro) IP WB Rat IgG2a Extracellular Domain Log in to see ICR10 2
0.0009004611 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
0.0009004611 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
0.0009004611 ABIN114788 IHC (fro) IHC (p) IP WB Mouse IgG1 Extracellular Domain Log in to see 111-6
0.0009004611 ABIN284399 ICC IP ELISA WB Mouse IgG1 kappa pTyr1173 Log in to see 9H2
0.0009004611 ABIN780370 ICC IHC (fro) FACS IP Mouse IgG2b Extracellular Domain Log in to see EGFR1 3
0.0009004611 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
0.0009004611 ABIN967696 IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
0.0009004611 ABIN967697 FACS IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
0.0009004611 ABIN1742583 IHC (fro) IHC (p) IP ELISA Mouse IgG1 AA 985-996 Log in to see 1

Top referenced anti-EGFR antibody for Immunoprecipitation

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 492 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 180 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 25 Antibodies
Immunocytochemistry (ICC) 67 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 151 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 232 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope Tyr992, pTyr992
(127), (56), (55), (52), (51), (49), (48), (45), (45), (44), (44), (33), (32), (30), (30), (25), (23), (23), (23), (22), (21), (19), (18), (17), (16), (16), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1700), (662), (590), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1264), (392), (36), (25), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(74), (57), (45), (35), (25), (25), (23), (22), (21), (21), (21), (21), (21), (21), (20), (2), (2), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(1102), (500), (380), (240), (232), (204), (174), (142), (86), (60), (55), (54), (25), (11), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN343716) Images
Specificity The antibody EM-12 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 992.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Synthetic phospho-peptide covering sequence around tyrosine 992 of human EGFR.
Clone EM-12
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN343716) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN343716) Images back to top
Application Notes Western Blotting: Recommended dilution: 1 µg/mL
Positive control: EGF-stimulated A431
Negative control: Non-stimulated A431

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN343716) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-EGFR Antibody (ABIN343716)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Daniele, Di Tullio, Santoro, Turacchio, De Matteis: "ARAP1 regulates EGF receptor trafficking and signalling." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 12, pp. 2221-35, 2009

Rappl, Piontek, Schlegel: "EGFR-dependent migration of glial cells is mediated by reorganisation of N-cadherin." in: Journal of cell science, Vol. 121, Issue Pt 24, pp. 4089-97, 2008

Moulder, Yakes, Muthuswamy, Bianco, Simpson, Arteaga: "Epidermal growth factor receptor (HER1) tyrosine kinase inhibitor ZD1839 (Iressa) inhibits HER2/neu (erbB2)-overexpressing breast cancer cells in vitro and in vivo." in: Cancer research, Vol. 61, Issue 24, pp. 8887-95, 2001

Batzer, Blaikie, Nelson, Schlessinger, Margolis: "The phosphotyrosine interaction domain of Shc binds an LXNPXY motif on the epidermal growth factor receptor." in: Molecular and cellular biology, Vol. 15, Issue 8, pp. 4403-9, 1995

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN343716) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (Tyr992) (ABIN343716) Western blotting analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12...
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (Tyr992) (ABIN343716) Immunoprecipitation ofEGFR from EGF-treated A431 cells by monoclonal antibodies (A), ...
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (Tyr992) (ABIN343716) Immunoprecipitation ofEGFR from EGF-treated A431 cells byphosphospecific monoclonal a...