anti-Human EGFR antibody for Immunoprecipitation

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
Tyr1173, pTyr1173
148
122
61
58
56
53
52
50
48
48
47
43
36
35
33
30
29
26
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
16
15
15
15
15
15
15
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2273
847
699
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1398
773
67
43
9
2
1
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
1324
608
561
487
438
272
222
221
109
84
66
56
35
24
19
14
11
8
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN343717
$ 381.15
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
22.267073 ABIN343716 FACS IP WB Mouse IgG1 Tyr992, pTyr992 Log in to see EM-12 4
22.267073 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
22.267073 ABIN269687 ICC FACS IF IP WB Mouse IgG2b Log in to see 6F1
22.267073 ABIN4307189 CyTOF FACS IP WB Mouse IgG1 pTyr1173 Log in to see EM-13
22.267073 ABIN4307190 CyTOF FACS IP WB Mouse IgG1 pTyr992 Log in to see EM-12
22.267073 ABIN4307285 ICC IF IHC IHC (p) IP WB Mouse IgG2b C-Term Log in to see 133
22.267073 ABIN2852742 IC IF IP WB Mouse Log in to see
1 ABIN98862 IP ELISA WB Rabbit Log in to see Polyclonal
1 ABIN452102 FACS IF IHC (p) IP WB Mouse IgG1 Log in to see H11 1
1 ABIN126767 EIA IF IP WB Mouse IgG1 pSer1071 Log in to see 1H9 1
1 ABIN118950 FACS IHC (fro) IP WB Rat IgG2a Extracellular Domain Log in to see ICR10 2
1 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN114788 IHC (fro) IHC (p) IP WB Mouse IgG1 Extracellular Domain Log in to see 111-6
1 ABIN152027 ICC IF IHC IHC (p) IP WB Rabbit AA 1100-1150 Log in to see Polyclonal 4
1 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Log in to see ICR10 1
1 ABIN967696 IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
1 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
1 ABIN967697 FACS IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
1 ABIN4899925 ELISA (Capture) FACS ICC IHC IP WB Goat IgG AA 25-645 Log in to see Polyclonal 6

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Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies
Dot Blot (DB) 84 Antibodies
ELISA 548 Antibodies
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General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope Tyr1173, pTyr1173
(148), (122), (61), (58), (56), (53), (52), (50), (48), (48), (47), (43), (36), (35), (33), (30), (29), (26), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2273), (847), (699), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1398), (773), (67), (43), (9), (2), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(1324), (608), (561), (487), (438), (272), (222), (221), (109), (84), (66), (56), (35), (24), (19), (14), (11), (8), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN343717) Images
Specificity The antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 1173.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.
Clone EM-13
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN343717) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling ProductDetails: References for anti-EGFR antibody (ABIN343717) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details ProductDetails: References for anti-EGFR antibody (ABIN343717) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

ProductDetails: References for anti-EGFR antibody (ABIN343717)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Gandhi, Zhang, Xie, Soh, Shigematsu, Zhang, Yamamoto, Peyton, Girard, Lockwood, Lam, Varella-Garcia, Minna, Gazdar: "Alterations in genes of the EGFR signaling pathway and their relationship to EGFR tyrosine kinase inhibitor sensitivity in lung cancer cell lines." in: PLoS ONE, Vol. 4, Issue 2, pp. e4576, 2009

Daniele, Di Tullio, Santoro, Turacchio, De Matteis: "ARAP1 regulates EGF receptor trafficking and signalling." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 12, pp. 2221-35, 2009

Rappl, Piontek, Schlegel: "EGFR-dependent migration of glial cells is mediated by reorganisation of N-cadherin." in: Journal of cell science, Vol. 121, Issue Pt 24, pp. 4089-97, 2008

Moulder, Yakes, Muthuswamy, Bianco, Simpson, Arteaga: "Epidermal growth factor receptor (HER1) tyrosine kinase inhibitor ZD1839 (Iressa) inhibits HER2/neu (erbB2)-overexpressing breast cancer cells in vitro and in vivo." in: Cancer research, Vol. 61, Issue 24, pp. 8887-95, 2001

Batzer, Blaikie, Nelson, Schlessinger, Margolis: "The phosphotyrosine interaction domain of Shc binds an LXNPXY motif on the epidermal growth factor receptor." in: Molecular and cellular biology, Vol. 15, Issue 8, pp. 4403-9, 1995

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN343717) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (Tyr1173) (ABIN343717) Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-1...
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (Tyr1173) (ABIN343717) Immunoprecipitation ofEGFR from EGF-treated A431 cells by monoclonal antibodies EM-12...
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (Tyr1173) (ABIN343717) Immunoprecipitation ofEGFR from EGF-treated A431 cells byphosphospecific monoclonal a...