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anti-Human EGFR antibody for Immunoprecipitation

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Reactivity
Human
2251
750
609
23
16
14
13
10
8
5
4
2
1
1
1
1
1
Host
Mouse
1399
750
69
45
8
2
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
96
76
66
59
48
46
25
24
23
23
23
22
21
21
21
21
21
21
21
21
21
21
21
21
20
19
18
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
1322
590
569
470
444
232
232
229
113
87
75
55
34
25
12
11
8
6
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2169502
$ 392.86
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.758226 ABIN2169503 ICC IP WB Mouse IgG1 Log in to see 6H11-2D11-G3
23.758226 ABIN343716 FACS IP WB Mouse IgG1 Tyr992, pTyr992 Log in to see EM-12 4
23.758226 ABIN343717 FACS IP WB Mouse IgG1 Tyr1173, pTyr1173 Log in to see EM-13 5
23.758226 ABIN2169500 ICC IP IHC WB Mouse IgG1 Log in to see 3F12-1H7-A10
23.758226 ABIN2169501 IP WB Mouse IgG1 Log in to see 1A11-G9-1E9
23.758226 ABIN269687 ICC FACS IF IP WB Mouse IgG2b Log in to see 6F1
23.758226 ABIN2854754 ICC FACS IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
23.758226 ABIN4307190 CyTOF FACS IP WB Mouse IgG1 pTyr992 Log in to see EM-12
23.758226 ABIN4307189 CyTOF FACS IP WB Mouse IgG1 pTyr1173 Log in to see EM-13
23.758226 ABIN2852742 IC IF IP WB Mouse Log in to see
23.758226 ABIN4307285 ICC IF IHC IHC (p) IP WB Mouse IgG2b C-Term Log in to see 133
1 ABIN98862 IP ELISA WB Rabbit Log in to see Polyclonal
1 ABIN452102 FACS IF IHC (p) IP WB Mouse IgG1 Log in to see H11 1
1 ABIN126767 EIA IF IP WB Mouse IgG1 pSer1071 Log in to see 1H9 1
1 ABIN118950 FACS IHC (fro) IP WB Rat IgG2a Extracellular Domain Log in to see ICR10 2
1 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
1 ABIN114788 IHC (fro) IHC (p) IP WB Mouse IgG1 Extracellular Domain Log in to see 111-6
1 ABIN152027 ICC IF IHC IHC (p) IP WB Rabbit AA 1100-1150 Log in to see Polyclonal 4

Top referenced anti-EGFR antibody for Immunoprecipitation

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Dot Blot (DB) 87 Antibodies
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Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
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Immunocytochemistry (ICC) 112 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2251), (750), (609), (23), (16), (14), (13), (10), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(1399), (750), (69), (45), (8), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(96), (76), (66), (59), (48), (46), (25), (24), (23), (23), (23), (22), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (20), (19), (18), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(1322), (590), (569), (470), (444), (232), (232), (229), (113), (87), (75), (55), (34), (25), (12), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity The antibody detects endogenous level of total EGFR.
Cross-Reactivity Monkey
Cross-Reactivity (Details) Does not cross-react with related proteins.
Purification Affinity purified
Immunogen Purified recombinant human EGFR protein fragments expressed in E.coli.
Clone 5E10-G10-E11
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Subunit structure: Binding of the ligand triggers homo- and/or heterodimerization of the receptor triggering its autophosphorylation. Heterodimer with ERBB2. Interacts with ERRFI1, inhibits dimerization of the kinase domain and autophosphorylation. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts with GRB2, an adapter protein coupling the receptor to downstream signaling pathways. Interacts with GAB2, involved in signaling downstream of EGFR. Interacts with STAT3, mediates EGFR downstream signaling in cell proliferation. Interacts with RIPK1, involved in NF-kappa-B activation. Interacts (autophosphorylated) with CBL, CBLB and CBLC, involved in EGFR ubiquitination and regulation. Interacts with SOCS5, regulates EGFR degradation through TCEB1- and TCEB2-mediated ubiquitination and proteasomal degradation. Interacts with PRMT5, methylates EGFR and enhances interaction with PTPN6. Interacts (phosphorylated) with PTPN6, inhibits EGFR-dependent activation of MAPK/ERK. Interacts with COPG1, essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2, this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts with PCNA, positively regulates PCNA. Interacts with PELP1. Interacts with MUC1. Interacts with AP2M1. Interacts with FER. May interact with EPS8, mediates EPS8 phosphorylation. Interacts (via SH2 domains) with GRB2, NCK1 and NCK2. Interacts with ATX2. Interacts with GAREM. Interacts (ubiquitinated) with ANKRD13A/B/D, the interaction is direct and may regulate EGFR internalization after EGF stimulation. Interacts with GPER1, the interaction occurs in an estrogen-dependent manner.
Subcellular location: Cell membrane, Single-pass type I membrane protein. Endoplasmic reticulum membrane, Single-pass type I membrane protein. Golgi apparatus membrane, Single-pass type I membrane protein. Nucleus membrane, Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). Isoform 2: Secreted.
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.
Molecular Weight 175 kDa
Gene ID 1956
NCBI Accession NM_005228
UniProt P00533
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Buffer PBS (pH 7.4) containing with 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169502) anti-Epidermal Growth Factor Receptor (EGFR) antibody
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169502) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 2)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN2169502) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 3)