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anti-Human EGFR antibody for Proximity Ligation Assay

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
C-Term
145
138
59
57
54
52
52
49
47
46
45
44
36
34
32
31
30
26
25
24
24
24
23
22
22
19
18
17
17
16
16
14
14
14
14
14
14
14
13
13
13
11
10
10
10
9
9
9
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2196
763
625
23
16
14
13
10
8
5
4
2
1
1
1
1
1
Host
Rabbit
1397
698
69
45
7
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
94
74
64
57
46
44
44
25
23
21
21
21
21
21
21
21
20
20
19
19
19
19
19
19
19
19
16
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1332
577
527
447
426
252
244
184
87
73
73
55
25
11
11
8
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN152028
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.69973534 ABIN515185 IF PLA WB Mouse AA 1-405, full length Log in to see Polyclonal 1
0.69973534 ABIN515186 PLA WB Rabbit AA 1-405, full length Log in to see Polyclonal
0.0008911806 ABIN1978673 IF IP IHC (p) PLA WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-EGFR antibody for Proximity Ligation Assay

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 136 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 67 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 204 Antibodies
Immunohistochemistry (IHC) 185 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 131 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 482 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 8 Antibodies
Blocking Reagent (BR) 6 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope C-Term
(145), (138), (59), (57), (54), (52), (52), (49), (47), (46), (45), (44), (36), (34), (32), (31), (30), (26), (25), (24), (24), (24), (23), (22), (22), (19), (18), (17), (17), (16), (16), (14), (14), (14), (14), (14), (14), (14), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2196), (763), (625), (23), (16), (14), (13), (10), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1397), (698), (69), (45), (7), (2)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(94), (74), (64), (57), (46), (44), (44), (25), (23), (21), (21), (21), (21), (21), (21), (21), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (16), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(1332), (577), (527), (447), (426), (252), (244), (184), (87), (73), (73), (55), (25), (11), (11), (8), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN152028) Images
Cross-Reactivity (Details) Reactivity with mouse EGFR is likely owing to high conservation of sequence between this ortholog,human EGFR at the site to which the epitope maps. Based on 100% sequence identity, this antibody is expected to react with Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset,Northern white-cheeked gibbon. (Not validated)
Purification Immunogen affinity purified
Immunogen A synthetic peptide which maps to a region between residues 1150 and the C-terminus (residue 1210) of human Epidermal Growth Factor Receptor using the numbering given in Swiss-Prot entry P00533 (GeneID 1956).

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN152028) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Gene Symbol: EGFR
Gene ID 1956, 13649
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN152028) Images back to top
Application Notes Western Blot 1:10000- 1:25000, Immunohistochemistry 1:500- 1:2000, Immunocytochemistry/Immunofluorescence 1:250-1:2000, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:500- 1:2000, Proximity Ligation Assay 1:1000-1:20000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN152028) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate buffer, pH 7 to 8
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-EGFR Antibody (ABIN152028)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Loubeau, Boudra, Maquaire, Lours-Calet, Beaudoin, Verrelle, Morel: "NPM1 silencing reduces tumour growth and MAPK signalling in prostate cancer cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e96293, 2014 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN152028) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN152028) Detection of Human EGFR by Western Blot and Immunoprecipitation. Samples: Whole cell ...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN152028) Western Blot: EGF R/ErbB1 Antibody [ABIN152028] - Whole cell lysate (5, 15 and 50 ug)...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN152028) Western Blot: EGF R/ErbB1 Antibody [ABIN152028] - Whole cell lysate (50 ug) from 293T...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN152028) Immunohistochemistry-Paraffin: EGF R/ErbB1 Antibody [ABIN152028] - FFPE section of hu...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN152028) Immunohistochemistry-Paraffin: EGF R/ErbB1 Antibody [ABIN152028] - Human placenta. An...
Proximity Ligation Assay (PLA) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN152028) Proximity Ligation Assay: EGF R/ErbB1 Antibody [ABIN152028] - The above image shows r...