anti-Mouse (Murine) EGFR antibody for Enzyme Immunoassay

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pSer1071
147
134
64
64
60
58
57
54
54
53
49
49
43
39
36
33
32
30
29
29
27
26
26
25
24
22
19
18
18
17
16
16
16
15
15
15
15
15
14
14
12
11
10
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
2444
865
700
28
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1462
867
92
44
11
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
103
80
71
64
58
56
34
34
28
28
28
28
28
28
28
27
27
26
26
26
24
21
21
21
21
21
20
19
18
4
2
2
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
1451
702
615
556
511
272
259
229
127
84
66
55
45
24
19
14
12
11
7
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126767
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN126765 EIA IP WB Mouse IgG1 pTyr1069 Log in to see 11C2
1 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN5551788 EIA IHC (p) WB Rabbit IgG AA 1189-1199, pTyr1197 Log in to see Polyclonal
1 ABIN5551776 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal
1 ABIN126786 EIA IF IP WB Mouse IgG1 C-Term Log in to see 13G8
1 ABIN126780 EIA IF IP WB Mouse IgG1 Tyr1197 Log in to see 20G3
1 ABIN5552236 EIA IHC (p) WB Rabbit Ig Fraction Ser1070 Log in to see Polyclonal
1 ABIN5556718 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal
1 ABIN318810 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal 1
1 ABIN492342 EIA IP WB Biotin Mouse IgG1 pTyr1069 Log in to see 11C2
1 ABIN492344 EIA IF IP WB Biotin Mouse IgG1 Tyr1197 Log in to see 20G3
1 ABIN126785 EIA IF IP WB Biotin Mouse IgG1 C-Term Log in to see 13G8
1 ABIN265396 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN372504 EIA IF IHC (p) IP WB Sheep IgG Log in to see Polyclonal
1 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126766 EIA IF IP WB Biotin Mouse IgG1 pSer1071 Log in to see 1H9

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pSer1071
(147), (134), (64), (64), (60), (58), (57), (54), (54), (53), (49), (49), (43), (39), (36), (33), (32), (30), (29), (29), (27), (26), (26), (25), (24), (22), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (12), (11), (10), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(2444), (865), (700), (28), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1462), (867), (92), (44), (11), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(103), (80), (71), (64), (58), (56), (34), (34), (28), (28), (28), (28), (28), (28), (28), (27), (27), (26), (26), (26), (24), (21), (21), (21), (21), (21), (20), (19), (18), (4), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(1451), (702), (615), (556), (511), (272), (259), (229), (127), (84), (66), (55), (45), (24), (19), (14), (12), (11), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN126767) Images
Specificity This antibody recognizes EGFR phosphorylated at Serine 1071.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHRemarks: Epitope: Phosphoserine 1071, R Y S pS D S T
Clone 1H9
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN126767) Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN126767) Images back to top
Application Notes Western blot. ELISA (0.1 μg/mL). Immunoprecipitation (1 - 10 g per 10^6 pervanadate-treated A431 cells). Immunoflourescence (0.1-1 μg/mL). Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN126767) Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze inliquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-EGFR antibody (ABIN126767)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Kondo, Higa-Nakamine, Maeda, Toku, Kakinohana, Sugahara, Kukita, Yamamoto: "Stimulation of Cell Migration by Flagellin Through the p38 MAP Kinase Pathway in Cultured Intestinal Epithelial Cells." in: Journal of cellular biochemistry, 2015

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN126767) back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody (ABIN126767) anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody