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anti-Mouse (Murine) EGFR antibody for Enzyme Immunoassay

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pSer1071
145
139
59
58
54
52
51
48
46
46
46
44
36
34
32
31
30
25
25
24
23
23
23
22
22
19
19
17
17
16
16
14
14
13
13
13
13
13
13
13
12
12
10
10
10
10
10
9
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
2251
749
609
22
16
14
13
10
8
5
4
2
1
1
1
1
1
Host
Mouse
1399
750
69
45
8
2
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
96
76
66
59
48
46
25
24
23
23
23
22
21
21
21
21
21
21
21
21
21
21
21
21
20
19
18
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
1322
590
569
470
444
232
232
228
114
87
75
54
34
25
12
11
8
6
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN126767
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN359838 EIA IHC (p) WB Rabbit Ig Ser1070 Log in to see Polyclonal 3
1 ABIN401341 EIA IHC (p) WB Rabbit IgG AA 1189-1199, pTyr1197 Log in to see Polyclonal 4
1 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
1 ABIN401323 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal 4
1 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126765 EIA IP WB Mouse IgG1 pTyr1069 Log in to see 11C2
1 ABIN126785 EIA IF IP WB Biotin Mouse IgG1 C-Term Log in to see 13G8
1 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN318810 EIA WB Rabbit IgG AA 1189-1199 Log in to see Polyclonal 1
1 ABIN126768 EIA IP WB Biotin Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126780 EIA IF IP WB Mouse IgG1 Tyr1197 Log in to see 20G3
1 ABIN126766 EIA IF IP WB Biotin Mouse IgG1 pSer1071 Log in to see 1H9
1 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126786 EIA IF IP WB Mouse IgG1 C-Term Log in to see 13G8
1 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN492342 EIA IP WB Biotin Mouse IgG1 pTyr1069 Log in to see 11C2
1 ABIN492344 EIA IF IP WB Biotin Mouse IgG1 Tyr1197 Log in to see 20G3
1 ABIN265396 EIA IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-EGFR antibody for Enzyme Immunoassay

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Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 4 Antibodies
ELISA 136 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 67 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 78 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 188 Antibodies
Immunohistochemistry (IHC) 186 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 132 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 482 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 8 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pSer1071
(145), (139), (59), (58), (54), (52), (51), (48), (46), (46), (46), (44), (36), (34), (32), (31), (30), (25), (25), (24), (23), (23), (23), (22), (22), (19), (19), (17), (17), (16), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (10), (10), (10), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(2251), (749), (609), (22), (16), (14), (13), (10), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(1399), (750), (69), (45), (8), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(96), (76), (66), (59), (48), (46), (25), (24), (23), (23), (23), (22), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (20), (19), (18), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(1322), (590), (569), (470), (444), (232), (232), (228), (114), (87), (75), (54), (34), (25), (12), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN126767) Images
Specificity This antibody recognizes EGFR phosphorylated at Serine 1071.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHRemarks: Epitope: Phosphoserine 1071, R Y S pS D S T
Clone 1H9
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN126767) Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN126767) Images back to top
Application Notes Western blot. ELISA (0.1 μg/mL). Immunoprecipitation (1 - 10 g per 10^6 pervanadate-treated A431 cells). Immunoflourescence (0.1-1 μg/mL). Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN126767) Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze inliquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-EGFR Antibody (ABIN126767)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Kondo, Higa-Nakamine, Maeda, Toku, Kakinohana, Sugahara, Kukita, Yamamoto: "Stimulation of Cell Migration by Flagellin Through the p38 MAP Kinase Pathway in Cultured Intestinal Epithelial Cells." in: Journal of cellular biochemistry, 2015

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN126767) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pSer1071) (ABIN126767) anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody