anti-Mouse (Murine) EGFR antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pTyr845
147
134
64
64
60
58
57
54
54
53
49
49
43
39
36
33
32
30
29
29
27
26
25
25
24
22
19
18
18
17
16
16
16
15
15
15
15
15
14
14
13
11
10
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2444
865
699
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1462
867
92
44
11
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
103
80
71
64
58
56
34
34
28
28
28
28
28
28
28
27
27
26
26
26
24
21
21
21
21
21
20
19
18
4
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), ELISA, Western Blotting (WB)
1451
702
614
556
511
272
260
230
127
84
66
56
45
24
19
14
12
11
7
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN967602
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr845
(147), (134), (64), (64), (60), (58), (57), (54), (54), (53), (49), (49), (43), (39), (36), (33), (32), (30), (29), (29), (27), (26), (25), (25), (24), (22), (19), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (10), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2444), (865), (699), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1462), (867), (92), (44), (11), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(103), (80), (71), (64), (58), (56), (34), (34), (28), (28), (28), (28), (28), (28), (28), (27), (27), (26), (26), (26), (24), (21), (21), (21), (21), (21), (20), (19), (18), (4), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), ELISA, Western Blotting (WB)
(1451), (702), (614), (556), (511), (272), (260), (230), (127), (84), (66), (56), (45), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN967602) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human EGF Receptor Peptide
Clone 12A3
Isotype IgG1, kappa

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN967602) Images back to top
Antigen
Alternative Name EGF Receptor (EGFR Antibody Abstract)
Background Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF Receptor (EGFR) binding and activation of intrinsic tyrosine kinase activity. EGFR, also known as ErbB1 or HER1, is a member of the ErbB class of receptor protein tyrosine kinases. It has an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic region containing a protein tyrosine kinase domain and a c-terminal regulatory domain with many phosphorylation sites. Following ligand binding, EGFR forms homodimers and heterodimers with ErbB2. Specific C-terminal tyrosine residues are then autophosphorylated and, in turn, bind to adaptor proteins, kinases, or protein tyrosine phosphatases. In addition, c-Src-dependent phosphorylations at other sites, such as tyrosine 845 (Y845) in the kinase domain, regulate the receptor's kinase activity. Inappropriate expression or mutations of EGFR and/or deregulation of its signaling pathways are associated with many types of cancer, making EGFR a promising target for cancer therapies.
The 12A3 monoclonal antibody recognizes the phosphorylated Y845 in the protein tyrosine kinase domain of human EGFR.
Molecular Weight 175 kDa
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN967602) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN967602) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-EGFR antibody (ABIN967602)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Hristozova, Konschak, Budach, Tinhofer: "A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 81, Issue 6, pp. 489-95, 2012

Boerner, Demory, Silva, Parsons: "Phosphorylation of Y845 on the epidermal growth factor receptor mediates binding to the mitochondrial protein cytochrome c oxidase subunit II." in: Molecular and cellular biology, Vol. 24, Issue 16, pp. 7059-71, 2004

Mendelsohn, Baselga: "Status of epidermal growth factor receptor antagonists in the biology and treatment of cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 21, Issue 14, pp. 2787-99, 2003

Moro, Dolce, Cabodi, Bergatto, Boeri Erba, Smeriglio, Turco, Retta, Giuffrida, Venturino, Godovac-Zimmermann, Conti, Schaefer, Beguinot, Tacchetti, Gaggini, Silengo, Tarone, Defilippi: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, 2002

Wu, Graves, Gill, Parsons, Samet: "Src-dependent phosphorylation of the epidermal growth factor receptor on tyrosine 845 is required for zinc-induced Ras activation." in: The Journal of biological chemistry, Vol. 277, Issue 27, pp. 24252-7, 2002

Olayioye, Neve, Lane, Hynes: "The ErbB signaling network: receptor heterodimerization in development and cancer." in: The EMBO journal, Vol. 19, Issue 13, pp. 3159-67, 2000

Biscardi, Maa, Tice, Cox, Leu, Parsons: "c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 8335-43, 1999

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN967602) back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr845) antibody (ABIN967602) Western blot analysis of EGF Receptor (pY845) in human epidermis. Lysates from contro...
 image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr845) antibody (ABIN967602) EGF Receptor (pY845) staining on tonsil. Fresh human tonsil was incubated in 5 mM Per...
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr845) antibody (ABIN967602) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr845) antibody (Image 3)