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anti-Mouse (Murine) EGFR antibody for Western Blotting

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pSer1071
127
56
55
52
51
49
48
45
45
44
44
33
32
30
30
25
23
23
23
22
21
19
18
17
16
16
14
13
13
13
13
13
13
13
12
11
10
10
10
9
9
9
8
8
8
8
8
7
7
7
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
1700
661
590
20
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1264
392
36
25
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
74
57
45
35
25
25
23
22
21
21
21
21
21
21
20
2
2
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
1102
500
380
240
232
204
175
141
86
60
54
54
25
11
9
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126767
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12226487 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
0.12226487 ABIN1532007 ELISA WB Rabbit IgG pSer1026 Log in to see Polyclonal 1
0.12226487 ABIN1532008 ELISA WB Rabbit IgG pSer1071 Log in to see Polyclonal 1
0.12226487 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
0.12226487 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
0.12226487 ABIN391943 IHC (p) WB Rabbit Ig AA 1048-1077, Ser1070 Log in to see Polyclonal 5
0.12226487 ABIN359838 EIA IHC (p) WB Rabbit Ig Ser1070 Log in to see Polyclonal 3
0.12226487 ABIN1533044 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12226487 ABIN1533045 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12226487 ABIN1532602 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12226487 ABIN1532852 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12226487 ABIN1533046 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12226487 ABIN967602 IHC (f) ELISA WB Mouse IgG1, kappa pTyr845 Log in to see 12A3 7
0.12226487 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
0.12226487 ABIN967696 IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
0.12226487 ABIN967697 FACS IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
0.12226487 ABIN1584389 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
0.12226487 ABIN126765 EIA IP WB Mouse IgG1 pTyr1069 Log in to see 11C2
0.12226487 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
0.12226487 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2

Top referenced anti-EGFR antibody for Western Blotting

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 135 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 188 Antibodies
Immunohistochemistry (IHC) 163 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 96 Antibodies
Immunoprecipitation (IP) 36 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 444 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pSer1071
(127), (56), (55), (52), (51), (49), (48), (45), (45), (44), (44), (33), (32), (30), (30), (25), (23), (23), (23), (22), (21), (19), (18), (17), (16), (16), (14), (13), (13), (13), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(1700), (661), (590), (20), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1264), (392), (36), (25), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(74), (57), (45), (35), (25), (25), (23), (22), (21), (21), (21), (21), (21), (21), (20), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(1102), (500), (380), (240), (232), (204), (175), (141), (86), (60), (54), (54), (25), (11), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN126767) Images
Specificity This antibody recognizes EGFR phosphorylated at Serine 1071.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Subsequent thiophilic adsorption and size exclusion chromatography
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHRemarks: Epitope: Phosphoserine 1071, R Y S pS D S T
Clone 1H9
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN126767) Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN126767) Images back to top
Application Notes Western blot. ELISA (0.1 μg/mL). Immunoprecipitation (1 - 10 g per 10^6 pervanadate-treated A431 cells). Immunoflourescence (0.1-1 μg/mL). Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN126767) Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze inliquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-EGFR Antibody (ABIN126767)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Kondo, Higa-Nakamine, Maeda, Toku, Kakinohana, Sugahara, Kukita, Yamamoto: "Stimulation of Cell Migration by Flagellin Through the p38 MAP Kinase Pathway in Cultured Intestinal Epithelial Cells." in: Journal of cellular biochemistry, 2015

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN126767) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pSer1071) (ABIN126767) anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody