anti-Rat (Rattus) EGFR antibody for Immunoprecipitation

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pTyr1173
148
122
61
58
56
53
52
50
48
48
47
43
36
35
33
30
29
26
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
16
15
15
15
15
15
15
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
2273
846
698
28
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1398
773
67
43
9
2
1
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1324
609
560
487
438
272
222
221
109
84
66
56
35
24
19
14
11
8
7
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN967603
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126768 EIA IP WB Biotin Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN266917 IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN372504 EIA IF IHC (p) IP WB Sheep IgG Log in to see Polyclonal
1 ABIN2704751 IP IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN5556718 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal
1 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN2736680 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN364308 ICC IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal
1 ABIN2471324 ICC IP IHC WB Sheep Internal Region Log in to see Polyclonal
1 ABIN2470777 ICC IF IP IHC ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN594843 ICC IP ELISA WB Mouse IgG1 AA 1165-1186 Log in to see
1 ABIN2692020 IP ELISA WB Rabbit IgG AA 1-646 Log in to see Polyclonal
1 ABIN615211 IF IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal
1 ABIN1733923 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN218581 IHC (p) IP ELISA WB Rabbit AA 1166-1180 Log in to see Polyclonal
1 ABIN217086 IP ELISA WB Rabbit Tyr1016 Log in to see Polyclonal
1 ABIN1733924 ICC IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal

Top referenced anti-EGFR antibody for Immunoprecipitation

Similar anti-EGFR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 143 Antibodies 161 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies 23 Antibodies
Flow Cytometry (FACS) 3 Antibodies 66 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 51 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 220 Antibodies
Immunohistochemistry (IHC) 160 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Western Blotting (WB) 453 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1173
(148), (122), (61), (58), (56), (53), (52), (50), (48), (48), (47), (43), (36), (35), (33), (30), (29), (26), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(2273), (846), (698), (28), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1398), (773), (67), (43), (9), (2), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1324), (609), (560), (487), (438), (272), (222), (221), (109), (84), (66), (56), (35), (24), (19), (14), (11), (8), (7), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN967603) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human EGF Receptor Peptide
Clone 9H2
Isotype IgG1, kappa

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN967603) Images back to top
Antigen
Alternative Name EGF Receptor (EGFR Antibody Abstract)
Background Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF Receptor (EGFR) binding and activation of intrinsic tyrosine kinase activity. EGFR, also known as ErbB1 or HER1, is a member of the ErbB class of receptor protein tyrosine kinases. It has an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic region containing a protein tyrosine kinase domain and a c-terminal regulatory domain with many phosphorylation sites. Following ligand binding, EGFR forms homodimers and heterodimers with ErbB2. Specific C-terminal tyrosine residues are then autophosphorylated and, in turn, bind to adaptor proteins, kinases, or protein tyrosine phosphatases. Specifically, phosphorylated tyrosine 1173 (Y1173) interacts with Shc, SHP1, and PLCg, which mediate downstream signaling cascades and negative feedback regulation of EGFR activation. Inappropriate expression or mutations of EGFR and/or deregulation of its signaling pathways are associated with many types of cancer, making EGFR a promising target for cancer therapies.
The 9H2 monoclonal antibody recognizes the phosphorylated Y1173 in the regulatory domain of human EGFR.
Molecular Weight 175 kDa
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling ProductDetails: References for anti-EGFR antibody (ABIN967603) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details ProductDetails: References for anti-EGFR antibody (ABIN967603) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-EGFR antibody (ABIN967603)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Hristozova, Konschak, Budach, Tinhofer: "A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 81, Issue 6, pp. 489-95, 2012

Mendelsohn, Baselga: "Status of epidermal growth factor receptor antagonists in the biology and treatment of cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 21, Issue 14, pp. 2787-99, 2003

Olayioye, Neve, Lane, Hynes: "The ErbB signaling network: receptor heterodimerization in development and cancer." in: The EMBO journal, Vol. 19, Issue 13, pp. 3159-67, 2000

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN967603) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1173) (ABIN967603) Western blot analysis of EGF Receptor (pY1173) in human epidermis (left figure). Lysa...
 image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1173) (ABIN967603) Immunohistochemical analysis for EGF Receptor (pY1173) staining on tonsil (right figu...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1173) (ABIN967603) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 3)
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr1173) (ABIN967603) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (Image 4)