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anti-Rat (Rattus) EGFR antibody for Western Blotting

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pSer1071
145
139
59
57
54
52
52
49
47
46
45
44
36
34
32
31
30
26
25
24
24
24
23
22
22
19
18
17
17
16
16
14
14
14
14
14
14
14
13
13
12
11
10
10
10
9
9
9
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2196
763
624
23
16
14
13
10
8
5
4
2
1
1
1
1
1
Host
Rabbit
1397
698
69
45
7
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
94
74
64
57
46
44
44
25
23
21
21
21
21
21
21
21
20
20
19
19
19
19
19
19
19
19
16
2
2
2
2
1
Application
ELISA, Western Blotting (WB)
1332
576
527
448
427
252
245
185
87
74
73
55
25
11
11
8
6
6
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN1532008
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12496077 ABIN1532007 ELISA WB Rabbit IgG pSer1026 Log in to see Polyclonal 1
0.12496077 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
0.12496077 ABIN1532602 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12496077 ABIN1532852 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12496077 ABIN1533044 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12496077 ABIN1533045 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12496077 ABIN1533046 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12496077 ABIN1584389 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
0.12496077 ABIN967602 IHC (f) ELISA WB Mouse IgG1, kappa pTyr845 Log in to see 12A3 7
0.12496077 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
0.12496077 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
0.12496077 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
0.12496077 ABIN126763 WB Mouse IgG1 AA 960-980 Log in to see 16F8
0.12496077 ABIN343626 ICC WB Rabbit IgG Log in to see Polyclonal 2
0.12496077 ABIN1531312 IF IHC ELISA WB Rabbit IgG pTyr1016 Log in to see Polyclonal
0.12496077 ABIN1531177 IF ELISA WB Rabbit IgG pThr678 Log in to see Polyclonal
0.12496077 ABIN1531178 IF IHC ELISA WB Rabbit IgG pThr693 Log in to see Polyclonal
0.12496077 ABIN1531585 ELISA WB Rabbit IgG pTyr1069 Log in to see Polyclonal
0.12496077 ABIN1531821 ELISA WB Rabbit IgG pTyr1172 Log in to see Polyclonal
0.12496077 ABIN1531818 IHC ELISA WB Rabbit IgG pSer1070 Log in to see Polyclonal

Top referenced anti-EGFR antibody for Western Blotting

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Radioimmunoassay (RIA) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies 1 Antibodies
Neutralization (Neut) 11 Antibodies
Luminex Assay (LMNX) 11 Antibodies
Intracellular Staining (ICS) 2 Antibodies 2 Antibodies 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
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Indirect Immunofluorescence Assay (IFA) 2 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pSer1071
(145), (139), (59), (57), (54), (52), (52), (49), (47), (46), (45), (44), (36), (34), (32), (31), (30), (26), (25), (24), (24), (24), (23), (22), (22), (19), (18), (17), (17), (16), (16), (14), (14), (14), (14), (14), (14), (14), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2196), (763), (624), (23), (16), (14), (13), (10), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1397), (698), (69), (45), (7), (2)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(94), (74), (64), (57), (46), (44), (44), (25), (23), (21), (21), (21), (21), (21), (21), (21), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (16), (2), (2), (2), (2), (1)
Application ELISA, Western Blotting (WB)
(1332), (576), (527), (448), (427), (252), (245), (185), (87), (74), (73), (55), (25), (11), (11), (8), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1532008) Images
Specificity EGFR (Phospho-Ser1071) Antibody detects endogenous levels of EGFR only when phosphorylated at Ser1071.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Ser1071.
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN1532008) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background Molecular weight of target protein: 134 kDa
Synonyms: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 , EGFR , ERBB1
Molecular Weight 134 kDa
Gene ID 1956
OMIM 131550
UniProt P00533
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN1532008) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.488293 (NCBI Gene Symbol: EGFR)

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN1532008) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-EGFR Antibody (ABIN1532008)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Ullrich, Coussens, Hayflick, Dull, Gray, Tam, Lee, Yarden, Libermann, Schlessinger: "Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells." in: Nature, Vol. 309, Issue 5967, pp. 418-25, 1984

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1532008) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pSer1071) (ABIN1532008) Western blot analysis of extracts from HepG2 cells treated with serum 20% 15', using ...