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|Application / Reactivity||Rat (Rattus)|
|Confocal Microscopy (CM)||4 Antibodies|
|Dot Blot (DB)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Neutralization (Neut)||3 Antibodies|
|Western Blotting (WB)||28 Antibodies|
|Antigen||Growth Hormone 1 (GH1) Antibodies|
|Epitope||AA 27-216 Alternatives|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This Growth Hormone 1 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-Growth Hormone 1 AntibodyTarget Details Growth Hormone 1 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Somatotropin(GH1) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Rat.
Gene Name: growth hormone 1
Protein Name: Somatotropin
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived rat GH recombinant protein(Position: F27-F216).|
Target Details Growth Hormone 1Product Details anti-Growth Hormone 1 Antibody Application Details Handling Images back to top
|Alternative Name||GH1 (GH1 Antibody Abstract)|
|Background||Growth Hormone(GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98 % amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.|
|Pathways||NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process|
Application DetailsProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Rat
Neut: Species: Rat
IP: Species: Rat
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Growth Hormone 1 Antibody Target Details Growth Hormone 1 Application Details Handling back to top