Human Growth Hormone 1 ELISA Kit

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Antigen
Growth Hormone 1 (GH1) ELISA Kits
  • ghl
  • GH
  • GH1
  • Lhrhr
  • Gh1
  • Gh
  • GH-N
  • GHN
  • IGHD1B
  • hGH-N
  • RNGHGP
  • CSH-4
  • gh
  • growth hormone 1
  • gonadotropin releasing hormone receptor
  • growth hormone
  • growth hormone prepeptide
  • Somatotropin-like
  • somatotropin-like
  • gh1
  • LOC100232594
  • GH1
  • Gnrhr
  • Gh
  • GH
  • Gh1
  • LOC100136588
  • LOC100356068
  • LOC100392546
Epitope
AA 27-217
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
26
9
8
8
8
5
4
4
4
3
3
3
1
1
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Options
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Catalog No. ABIN411378
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.76768 ABIN624985 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 4-600 pg/mL 4 pg/mL Log in to see
9.76768 ABIN1115304 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
9.76768 ABIN1305170 Sandwich ELISA Serum 0.087-24.0 ng/mL 0.087 ng/mL Log in to see
9.76768 ABIN2708231 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
9.76768 ABIN2114952 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
1 ABIN367593 Sandwich ELISA Serum, Plasma 2.5-50 ng/mL 2.5 ng/mL Log in to see
1 ABIN455267 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN509672 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
1 ABIN4947624 Competition ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 123.5-10.000 pg/mL 123.5 pg/mL Log in to see
1 ABIN649033 Sandwich ELISA Log in to see 5
1 ABIN5520680 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 25-1600 pg/mL 25 pg/mL Log in to see
1 ABIN2042489 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1846925 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1995298 Sandwich ELISA Plasma Log in to see
1 ABIN5068170 Sandwich ELISA Serum Log in to see
1 ABIN2534153 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 48-2800 pg/mL 48 pg/mL Log in to see
1 ABIN5068345 Sandwich ELISA Plasma, Serum Log in to see
1 ABIN2948067 0.313-20 ng/mL 0.313 ng/mL Log in to see
1 ABIN2948066 0.313-20 ng/mL 0.313 ng/mL Log in to see
1 ABIN2116007 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see

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General

Antigen Growth Hormone 1 (GH1) ELISA Kits
Epitope AA 27-217
Reactivity Human
Kits with alternative reactivity to:
(26), (9), (8), (8), (8), (5), (4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Growth Hormone 1 ELISA Kit

Target details Application Details Handling ProductDetails: References for Growth Hormone 1 Kit (ABIN411378) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Growth Hormone
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: E.coli
Immunogen sequence: F27-F217
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: F27-F217

Target details

Product Details Growth Hormone 1 ELISA Kit Application Details Handling ProductDetails: References for Growth Hormone 1 Kit (ABIN411378) Images back to top
Antigen
Alternative Name GH1 (GH1 ELISA Kit Abstract)
Background

Background: Growth hormone(GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone(GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency(GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.

Synonyms: Growth hormone 1 isoform 1 ,HCG1749481, isoform CRA_s ,GH1 ,hCG_1749481 ,

Full Gene Name: growth hormone 1

Cellular Localisation: Secreted.
Gene ID 2688
UniProt B1A4G6
Pathways NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process

Application Details

Product Details Growth Hormone 1 ELISA Kit Target details Handling ProductDetails: References for Growth Hormone 1 Kit (ABIN411378) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated
Protocol human GH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH has been precoated onto 96-well plates. Standards(E.coli, F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GH amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human GH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human GH standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 101, Standard deviation: 4.44, CV(%): 4.4
  • Sample 2: n=16, Mean(pg/ml): 335, Standard deviation: 12.06, CV(%): 3.6
  • Sample 3: n=16, Mean(pg/ml): 618, Standard deviation: 24.10, CV(%): 3.9,
  • Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 9.922, CV(%): 8.2
  • Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 26.55, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 596, Standard deviation: 43.51, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details Growth Hormone 1 ELISA Kit Target details Application Details ProductDetails: References for Growth Hormone 1 Kit (ABIN411378) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for Growth Hormone 1 Kit (ABIN411378)

Product Details Growth Hormone 1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Baum, Katznelson, Sherman, Biller, Hayden, Schoenfeld, Cannistraro, Klibanski: "Effects of physiological growth hormone (GH) therapy on cognition and quality of life in patients with adult-onset GH deficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 83, Issue 9, pp. 3184-9, 1998

Owerbach, Rutter, Martial, Baxter, Shows: "Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans." in: Science (New York, N.Y.), Vol. 209, Issue 4453, pp. 289-92, 1980

Niall, Hogan, Sauer, Rosenblum, Greenwood: "Sequences of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 68, Issue 4, pp. 866-70, 1971

Images

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Supplier Images
ELISA image for Growth Hormone 1 ELISA Kit (GH1) (ABIN411378) Human Growth Hormone PicoKine ELISA Kit standard curve