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Human Growth Hormone 1 ELISA Kit

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Antigen
Growth Hormone 1 (GH1) ELISA Kits
  • CSH-4
  • gh
  • GH
  • Gh
  • GH-N
  • Gh1
  • GH1
  • ghl
  • GHN
  • hGH-N
  • IGHD1B
  • Lhrhr
  • RNGHGP
Epitope
AA 27-217
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
32
14
14
10
10
10
9
6
5
5
4
4
3
2
2
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN411378
$ 430.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN414380 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.30628014 ABIN1115304 Sandwich ELISA Serum, Plasma, Biological Fluids 0.313-20 ng/mL 0.31 ng/mL Log in to see
0.30628014 ABIN1305170 Sandwich ELISA Serum 0.087-24.0 ng/mL 0.087 ng/mL Log in to see
0.30628014 ABIN1028764 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.30628014 ABIN2708231 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.30628014 ABIN2114952 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012155735 ABIN367593 Sandwich ELISA Serum, Plasma 2.5-50 ng/mL 2.5 ng/mL Log in to see
0.0012155735 ABIN3032927 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.5-600 pg/mL 2.5 pg/mL Log in to see
0.0012155735 ABIN509672 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
0.0012155735 ABIN3198541 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 62.50-4000 pg/mL 62.50 pg/mL Log in to see
0.0012155735 ABIN455267 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN649033 Log in to see 5
0.0012155735 ABIN624985 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012155735 ABIN1112615 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2684359 Sandwich ELISA Serum 0.1-12 ng/mL 0.1 ng/mL Log in to see 2
0.0012155735 ABIN2042489 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2762219 Serum, Plasma 2.5-50 ng/mL 2.5 ng/mL Log in to see
0.0012155735 ABIN2064303 Serum Log in to see
0.0012155735 ABIN2684360 Sandwich ELISA Plasma (ACD), Plasma (heparin), Serum 1-50 mIU/L 1 mIU/L Log in to see
0.0012155735 ABIN1846925 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced Growth Hormone 1 ELISA Kit for ELISA

Similar Growth Hormone 1 ELISA Kits

Application / Reactivity Human
ELISA 30 ELISA Kits

General

Antigen Growth Hormone 1 (GH1) ELISA Kits
Epitope AA 27-217
Reactivity Human
Kits with alternative reactivity to:
(32), (14), (14), (10), (10), (10), (9), (6), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
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Product Details Growth Hormone 1 ELISA Kit

Target details Application Details Handling References for Growth Hormone 1 Kit (ABIN411378) Images
Purpose For quantitative detection of human GH in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human GH
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human Growth Hormone antibod - 1
  • Lyophilized recombinant human Growth Hormone standar - 10ng/tubex2
  • Biotinylated anti- human Growth Hormone antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: F27-F217

Target details

Product Details Growth Hormone 1 ELISA Kit Application Details Handling References for Growth Hormone 1 Kit (ABIN411378) Images back to top
Antigen
Alternative Name GH1 (GH1 ELISA Kit Abstract)
Background Growth hormone(GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone(GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency(GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
Gene ID 2688
UniProt B1A4G6
Pathways NF-kappaB Signaling, JAK-STAT Signaling

Application Details

Product Details Growth Hormone 1 ELISA Kit Target details Handling References for Growth Hormone 1 Kit (ABIN411378) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human GH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GH has been precoated onto 96-well plates. Standards(E.coli, F27-F217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GH amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human GH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human GH standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 101, Standard deviation: 4.44, CV(%): 4.4
  • Sample 2: n=16, Mean(pg/ml): 335, Standard deviation: 12.06, CV(%): 3.6
  • Sample 3: n=16, Mean(pg/ml): 618, Standard deviation: 24.10, CV(%): 3.9,
  • Sample 1: n=24, Mean(pg/ml): 121, Standard deviation: 9.922, CV(%): 8.2
  • Sample 2: n=24, Mean(pg/ml): 354, Standard deviation: 26.55, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 596, Standard deviation: 43.51, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details Growth Hormone 1 ELISA Kit Target details Application Details References for Growth Hormone 1 Kit (ABIN411378) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Growth Hormone 1 Kit (ABIN411378)

Product Details Growth Hormone 1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Baum, Katznelson, Sherman, Biller, Hayden, Schoenfeld, Cannistraro, Klibanski: "Effects of physiological growth hormone (GH) therapy on cognition and quality of life in patients with adult-onset GH deficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 83, Issue 9, pp. 3184-9, 1998

Owerbach, Rutter, Martial, Baxter, Shows: "Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans." in: Science (New York, N.Y.), Vol. 209, Issue 4453, pp. 289-92, 1980

Niall, Hogan, Sauer, Rosenblum, Greenwood: "Sequences of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 68, Issue 4, pp. 866-70, 1971

Images

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Supplier Images
ELISA image for Growth Hormone 1 ELISA Kit (GH1) (ABIN411378) Human Growth Hormone PicoKine ELISA Kit standard curve