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|Application / Reactivity||Human|
|Dot Blot (DB)||243 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||44 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||62 Antibodies|
|Immunohistochemistry (IHC)||85 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||49 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies|
|Proximity Ligation Assay (PLA)||6 Antibodies|
|Western Blotting (WB)||231 Antibodies|
|Antigen||Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies|
|Conjugate||This IKBKB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-IKBKB AntibodyTarget Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN358141) Images
|Specificity||This antibody detects IKKb pTyr199. Predicted to cross react with Mouse (100 % Antigen Homology).|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.|
Target Details IKBKBProduct Details anti-IKBKB Antibody Application Details Handling References for anti-IKBKB Antibody (ABIN358141) Images back to top
|Alternative Name||IKBKB / IKKB (IKBKB Antibody Abstract)|
|Background||NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta|
|Molecular Weight||86564 Da|
|Gene ID||3551, 9606|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-IKBKB Antibody Target Details IKBKB Handling References for anti-IKBKB Antibody (ABIN358141) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IKBKB Antibody Target Details IKBKB Application Details References for anti-IKBKB Antibody (ABIN358141) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IKBKB Antibody (ABIN358141)Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Tomasoni, Aiello, Cassis et al.: "Dendritic cells genetically engineered with adenoviral vector encoding dnIKK2 induce the formation of potent CD4+ T-regulatory cells." in: Transplantation, Vol. 79, Issue 9, pp. 1056-61, 2005 (PubMed).
Lappas, Yee, Permezel et al.: "Sulfasalazine and BAY 11-7082 interfere with the nuclear factor-kappa B and I kappa B kinase pathway to regulate the release of proinflammatory cytokines from human adipose tissue and skeletal muscle ..." in: Endocrinology, Vol. 146, Issue 3, pp. 1491-7, 2005 (PubMed).
Ea, Sun, Inoue et al.: "TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 43, pp. 15318-23, 2004 (PubMed).
May, Larsen, Shim et al.: "A novel ubiquitin-like domain in IkappaB kinase beta is required for functional activity of the kinase." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 45528-39, 2004 (PubMed).
Cárcamo, Pedraza, Bórquez-Ojeda et al.: "Vitamin C is a kinase inhibitor: dehydroascorbic acid inhibits IkappaBalpha kinase beta." in: Molecular and cellular biology, Vol. 24, Issue 15, pp. 6645-52, 2004 (PubMed).
ImagesProduct Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN358141) back to top