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anti-Human IKBKB antibody for Enzyme Immunoassay

Recommended IKBKB Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
  • AI132552
  • AIM-1
  • anon-89Bd
  • CG4201
  • DIK
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • DLAK
  • dLak
  • Dmel\\CG4201
  • DmIKK
  • Dmikkb
  • DmIKKb
  • DmIkkbeta
  • dmIKKbeta
  • Dmikkbeta
  • DmIKKbeta
  • DmIKKBeta
  • Ik
  • IK
  • IKBKB
  • ikk
  • IKK
  • IKK-2
  • IKK-beta
  • ikk-beta
  • ikk2
  • IKK2
  • Ikkb
  • IKKB
  • IKKb
  • ikkb
  • ikkbeta
  • Ikkbeta
  • IKKbeta
  • IKK[b]
  • ird
  • IRD-5
  • Ird-5
  • Ird5
  • IRD5
  • ird5/DmIkk[beta]
  • Lak
  • LRK
  • NFKBIKB
Epitope
pTyr199
47
31
26
21
21
21
20
20
20
20
19
19
19
19
19
19
19
14
14
14
13
9
8
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
597
152
122
16
10
2
2
1
1
Host
Rabbit
543
68
9
2
Clonality
Polyclonal
Conjugate
This IKBKB antibody is un-conjugated
36
36
36
31
31
31
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
296
264
231
82
58
47
43
22
14
5
5
3
3
2
1
1
Supplier
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Catalog No. ABIN358141
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN499972 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.0008833851 ABIN356415 EIA WB Mouse IgG1 Log in to see 62AT216
0.0008833851 ABIN1107723 EIA WB Mouse IgG1 Log in to see 10A2 1
0.0008833851 ABIN303371 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.0008833851 ABIN116875 EIA IP WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-IKBKB antibody for Enzyme Immunoassay

Similar anti-IKBKB Antibodies

Application / Reactivity Human
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ELISA 282 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 44 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 58 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 23 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies
Western Blotting (WB) 231 Antibodies

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
Epitope pTyr199
(47), (31), (26), (21), (21), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (14), (14), (14), (13), (9), (8), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(597), (152), (122), (16), (10), (2), (2), (1), (1)
Host Rabbit
(543), (68), (9), (2)
Clonality
Polyclonal
Conjugate This IKBKB antibody is un-conjugated
(36), (36), (36), (31), (31), (31), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(296), (264), (231), (82), (58), (47), (43), (22), (14), (5), (5), (3), (3), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-IKBKB Antibody

Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN358141) Images
Specificity This antibody detects IKKb pTyr199. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y199 of human IKKb.
Isotype Ig

Target Details IKBKB

Product Details anti-IKBKB Antibody Application Details Handling References for anti-IKBKB Antibody (ABIN358141) Images back to top
Antigen
Alternative Name IKBKB / IKKB (IKBKB Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta
Molecular Weight 86564 Da
Gene ID 3551, 9606
UniProt O14920
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-IKBKB Antibody Target Details IKBKB Handling References for anti-IKBKB Antibody (ABIN358141) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details References for anti-IKBKB Antibody (ABIN358141) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-IKBKB Antibody (ABIN358141)

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Background publications

Tomasoni, Aiello, Cassis, Noris, Longaretti, Cavinato, Azzollini, Pezzotta, Remuzzi, Benigni: "Dendritic cells genetically engineered with adenoviral vector encoding dnIKK2 induce the formation of potent CD4+ T-regulatory cells." in: Transplantation, Vol. 79, Issue 9, pp. 1056-61, 2005

Lappas, Yee, Permezel, Rice et al.: "Sulfasalazine and BAY 11-7082 interfere with the nuclear factor-kappa B and I kappa B kinase pathway to regulate the release of proinflammatory cytokines from human adipose tissue and skeletal muscle ..." in: Endocrinology, Vol. 146, Issue 3, pp. 1491-7, 2005

Cárcamo, Pedraza, Bórquez-Ojeda, Zhang, Sanchez, Golde: "Vitamin C is a kinase inhibitor: dehydroascorbic acid inhibits IkappaBalpha kinase beta." in: Molecular and cellular biology, Vol. 24, Issue 15, pp. 6645-52, 2004

May, Larsen, Shim, Madge, Ghosh: "A novel ubiquitin-like domain in IkappaB kinase beta is required for functional activity of the kinase." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 45528-39, 2004

Ea, Sun, Inoue, Chen: "TIFA activates IkappaB kinase (IKK) by promoting oligomerization and ubiquitination of TRAF6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 43, pp. 15318-23, 2004

Images

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN358141) back to top
Supplier Images
 image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (pTyr199) (ABIN358141) anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (pTyr199) antibody