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|Application / Reactivity||Human|
|Dot Blot (DB)||243 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||14 Antibodies|
|Immunofluorescence (IF)||44 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||62 Antibodies|
|Immunohistochemistry (IHC)||85 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||49 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies|
|Proximity Ligation Assay (PLA)||6 Antibodies|
|Western Blotting (WB)||231 Antibodies|
|Antigen||Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies|
|Conjugate||This IKBKB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-IKBKB AntibodyTarget Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN499972) Images
|Specificity||IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid. This polyclonal antibody has no cross response to IKKa or IKKy.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human IKK beta (C-Terminus 3) Peptide|
Target Details IKBKBProduct Details anti-IKBKB Antibody Application Details Handling References for anti-IKBKB Antibody (ABIN499972) Images back to top
|Alternative Name||IKBKB / IKKB (IKBKB Antibody Abstract)|
|Background||Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IkappaB degradation and NF-kappaB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKalpha and IKKbeta interact with each other and both are essential for NF-kappaB activation. IKKbeta phosphorylates both IkappaB-alpha and IkappaB-beta. IKKbeta is expressed in variety of human tissues.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-IKBKB Antibody Target Details IKBKB Handling References for anti-IKBKB Antibody (ABIN499972) Images back to top
ELISA. Wesern Blot: 1 - 2 μg/mL. A 87 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IKBKB Antibody Target Details IKBKB Application Details References for anti-IKBKB Antibody (ABIN499972) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-IKBKB Antibody (ABIN499972)Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Woronicz, Gao, Cao et al.: "IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 866-9, 1997 (PubMed).
Zandi, Rothwarf, Delhase et al.: "The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation." in: Cell, Vol. 91, Issue 2, pp. 243-52, 1997 (PubMed).
DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).
Régnier, Song, Gao et al.: "Identification and characterization of an IkappaB kinase." in: Cell, Vol. 90, Issue 2, pp. 373-83, 1997 (PubMed).
ImagesProduct Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN499972) back to top