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anti-Human IKBKB antibody for Immunoprecipitation

Recommended IKBKB Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
  • AI132552
  • AIM-1
  • anon-89Bd
  • CG4201
  • DIK
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • DLAK
  • dLak
  • Dmel\\CG4201
  • DmIKK
  • Dmikkb
  • DmIKKb
  • DmIkkbeta
  • dmIKKbeta
  • Dmikkbeta
  • DmIKKbeta
  • DmIKKBeta
  • Ik
  • IK
  • IKBKB
  • ikk
  • IKK
  • IKK-2
  • IKK-beta
  • ikk-beta
  • ikk2
  • IKK2
  • Ikkb
  • IKKB
  • IKKb
  • ikkb
  • ikkbeta
  • Ikkbeta
  • IKKbeta
  • IKK[b]
  • ird
  • IRD-5
  • Ird-5
  • Ird5
  • IRD5
  • ird5/DmIkk[beta]
  • Lak
  • LRK
  • NFKBIKB
Epitope
AA 1-666
48
32
26
21
21
21
20
20
20
20
19
19
19
19
19
19
19
14
14
14
13
9
8
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
642
192
126
16
10
2
2
1
1
Host
Mouse
550
106
9
2
Clonality
Monoclonal
Conjugate
This IKBKB antibody is un-conjugated
38
36
36
33
31
31
9
7
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
300
273
264
105
70
58
56
49
39
20
6
5
4
3
3
2
1
1
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Catalog No. ABIN967355
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN208072 IP WB Mouse IgG Log in to see 10A9B6
0.00089556724 ABIN2706368 IP IHC WB Rabbit pTyr199 Log in to see Polyclonal
0.00089556724 ABIN333168 IP ELISA WB Mouse IgG2b, kappa Log in to see
0.00089556724 ABIN208069 FACS IP WB Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN218607 IF IHC (p) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN303371 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN228561 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089556724 ABIN2668915 IP WB Mouse IgG1 Log in to see 10A9B6
0.00089556724 ABIN2470337 IF IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN220388 IP ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089556724 ABIN116875 EIA IP WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN4324264 FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324269 FACS IHC IHC (p) IP WB Allophycocyanin Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324270 FACS IHC IHC (p) IP WB DyLight 650 Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324271 FACS IHC IHC (p) IP WB DyLight 680 Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324272 FACS IHC IHC (p) IP WB DyLight 488 Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324274 FACS IHC IHC (p) IP WB DyLight 755 Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324275 FACS IHC IHC (p) IP WB PerCP Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324276 FACS IHC IHC (p) IP WB PE Mouse IgG1 Log in to see 10AG2
0.00089556724 ABIN4324277 FACS IHC IHC (p) IP WB DyLight 550 Mouse IgG1 Log in to see 10AG2

Top referenced anti-IKBKB antibody for Immunoprecipitation

Similar anti-IKBKB Antibodies

Application / Reactivity Human
Dot Blot (DB) 243 Antibodies
ELISA 285 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 39 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 49 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 58 Antibodies
Immunohistochemistry (IHC) 105 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 69 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies
Western Blotting (WB) 271 Antibodies

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
Epitope AA 1-666
(48), (32), (26), (21), (21), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (19), (14), (14), (14), (13), (9), (8), (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(642), (192), (126), (16), (10), (2), (2), (1), (1)
Host Mouse
(550), (106), (9), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IKBKB antibody is un-conjugated
(38), (36), (36), (33), (31), (31), (9), (7), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(300), (273), (264), (105), (70), (58), (56), (49), (39), (20), (6), (5), (4), (3), (3), (2), (1), (1)
Pubmed 12 references available
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Product Details anti-IKBKB Antibody

Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN967355) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IKKbeta recombinant protein aa. 1-666
Clone F18-1875
Isotype IgG1

Target Details IKBKB

Product Details anti-IKBKB Antibody Application Details Handling References for anti-IKBKB Antibody (ABIN967355) Images back to top
Antigen
Alternative Name IKK beta (IKBKB Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF-kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Experiments done on mice lacking IKKbeta demonstrate that these mice died at mid-gestation from uncontrolled liver apoptosis. Furthermore, when cells from IKKbeta-deficient m ice were treated with tumor necrosis factor alpha or interleukin 1, neither IKK nor NF-kappaB were activated. Thus IKKbeta, but not IKKalpha, plays a pivotal role in the induction of NF-kappaB and activation of IKK. IKKbeta migrates at ~87 kDa in SDS/PAGE. The antibody recognizes human IKKbeta. A recombinant protein corresponding to amino acids 1-666 of human IKKbeta was used as the immunogen.
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-IKBKB Antibody Target Details IKBKB Handling References for anti-IKBKB Antibody (ABIN967355) Images back to top
Application Notes Applications include western blot analysis (0.06 - 0.25 µg/ml). Other applications not routinely tested include immunoprecipitation (4 µg/sample). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (store lysate at -20°C). HeLa control lysate (ABIN968535) is sold separately as a ready-to-use western blot control.
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only

Handling

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details References for anti-IKBKB Antibody (ABIN967355) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-IKBKB Antibody (ABIN967355)

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Product cited in:

Israël: "The IKK complex: an integrator of all signals that activate NF-kappaB?" in: Trends in cell biology, Vol. 10, Issue 4, pp. 129-33, 2000

Chu, Ostertag, Li, Chang, Chen, Hu, Williams, Perrault, Karin: "JNK2 and IKKbeta are required for activating the innate response to viral infection." in: Immunity, Vol. 11, Issue 6, pp. 721-31, 2000

Li, Van Antwerp, Mercurio, Lee, Verma: "Severe liver degeneration in mice lacking the IkappaB kinase 2 gene." in: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 321-5, 1999

Li, Chu, Hu, Delhase, Deerinck, Ellisman, Johnson, Karin: "The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1839-45, 1999

Tanaka, Fuentes, Yamaguchi, Durnin, Dalrymple, Hardy, Goeddel: "Embryonic lethality, liver degeneration, and impaired NF-kappa B activation in IKK-beta-deficient mice." in: Immunity, Vol. 10, Issue 4, pp. 421-9, 1999

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998

Lee, Hagler, Chen, Maniatis: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." in: Cell, Vol. 88, Issue 2, pp. 213-22, 1997

Malinin, Boldin, Kovalenko, Wallach: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." in: Nature, Vol. 385, Issue 6616, pp. 540-4, 1997

Background publications

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997

Images

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling References for anti-IKBKB Antibody (ABIN967355) back to top
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (AA 1-666) (ABIN967355) Western blot analysis of Ikkbeta. Lysate from HeLa cells was probed with anti-Ikkbeta...