anti-Human IKBKB antibody for Western Blotting

Recommended IKBKB Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
  • ikk-beta
  • ikk2
  • ikkb
  • ikkbeta
  • IKBKB
  • IKKB
  • CG4201
  • DIK
  • DLAK
  • DmIKK
  • DmIKKBeta
  • DmIKKb
  • DmIKKbeta
  • DmIkkbeta
  • Dmel\\CG4201
  • Dmikkb
  • Dmikkbeta
  • IK
  • IKK
  • IKK-beta
  • IKKb
  • IKKbeta
  • IRD-5
  • IRD5
  • Ik
  • Ikkb
  • Ikkbeta
  • Ird-5
  • Ird5
  • LRK
  • Lak
  • anon-89Bd
  • dIKK
  • dIKK-beta
  • dIKKbeta
  • dLak
  • dmIKKbeta
  • ikk
  • ird
  • ird5/DmIkk[beta]
  • IKK2
  • NFKBIKB
  • AIM-1
  • AI132552
  • IKK-2
  • IKK[b]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
  • immune response deficient 5
  • inhibitor of kappaB kinase beta
  • ikbkb
  • IKBKB
  • ird5
  • Ikbkb
Epitope
Middle Region
51
33
29
23
21
21
20
20
20
20
20
20
20
19
19
19
19
17
17
17
15
8
8
8
7
6
6
5
5
4
4
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
678
229
160
21
13
7
6
5
4
3
1
1
Host
Rabbit
572
120
9
2
Clonality
Polyclonal
Conjugate
This IKBKB antibody is un-conjugated
38
37
37
33
31
31
9
7
5
5
5
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
326
297
264
100
80
76
68
41
37
34
6
6
6
5
3
3
2
2
1
Options
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Catalog No. ABIN2779400
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3140206 ABIN2779787 WB Rabbit N-Term Log in to see Polyclonal 1
3.3140206 ABIN252473 CyTOF FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 Log in to see 10AG2 57
3.3140206 ABIN252477 FACS GS ICC IF IP SimWes WB Mouse IgG1 kappa Log in to see 10A9B6 9
3.3140206 ABIN1882049 WB Mouse IgG1 full length Log in to see 62AT992 7
3.3140206 ABIN387826 WB Mouse IgG1 full length Log in to see 62AT216 11
3.3140206 ABIN967355 IP WB Mouse IgG1 AA 1-666 Log in to see F18-1875 12
3.3140206 ABIN269739 IHC (p) IHC ELISA WB Rabbit C-Term Log in to see Polyclonal 1
3.3140206 ABIN1882097 IF IHC (p) WB Rabbit Log in to see Polyclonal 8
3.3140206 ABIN2668916 WB Mouse IgG1 Log in to see 10AG2 1
3.3140206 ABIN3042734 IHC (p) WB Rabbit IgG AA 398-756 Log in to see Polyclonal
3.3140206 ABIN969212 ELISA WB Mouse IgG1 Log in to see 10A2 4
3.3140206 ABIN4324266 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.3140206 ABIN389574 WB Rabbit Ig pTyr199 Log in to see Polyclonal
3.3140206 ABIN3042938 WB Rabbit IgG AA 735-750, C-Term Log in to see Polyclonal
3.3140206 ABIN356415 EIA WB Mouse IgG1 Log in to see 62AT216
3.3140206 ABIN2856162 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.3140206 ABIN1531286 IHC ELISA WB Rabbit IgG pTyr188 Log in to see Polyclonal
3.3140206 ABIN1531325 IHC ELISA WB Rabbit IgG pTyr199 Log in to see Polyclonal
3.3140206 ABIN1724659 IHC ELISA WB Mouse IgG1 Log in to see 10A2C5B3 2
3.3140206 ABIN4324261 IHC IHC (p) WB Rabbit Log in to see Polyclonal 2

Top referenced anti-IKBKB antibody for Western Blotting

Similar anti-IKBKB Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Rabbit Guinea Pig Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 4 Antibodies 3 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies
Western Blotting (WB) 294 Antibodies 125 Antibodies 85 Antibodies 16 Antibodies 3 Antibodies 5 Antibodies 4 Antibodies 4 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies
Immunoprecipitation (IP) 33 Antibodies 21 Antibodies 10 Antibodies

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies
Epitope Middle Region
(51), (33), (29), (23), (21), (21), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (17), (17), (17), (15), (8), (8), (8), (7), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(678), (229), (160), (21), (13), (7), (6), (5), (4), (3), (1), (1)
Host Rabbit
(572), (120), (9), (2)
Clonality
Polyclonal
Conjugate This IKBKB antibody is un-conjugated
(38), (37), (37), (33), (31), (31), (9), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(326), (297), (264), (100), (80), (76), (68), (41), (37), (34), (6), (6), (6), (5), (3), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-IKBKB Antibody

Target Details IKBKB Application Details Handling ProductDetails: References for anti-IKBKB antibody (ABIN2779400) Images
Blocking Peptide Blocking peptide for this product available: ABIN978456
Sequence ITYETQISPR PQPESVSCIL QEPKRNLAFF QLRKVWGQVW HSIQTLKEDC
Predicted Reactivity Cow: 79%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against IKBKB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human IKBKB

Target Details IKBKB

Product Details anti-IKBKB Antibody Application Details Handling ProductDetails: References for anti-IKBKB antibody (ABIN2779400) Images back to top
Antigen
Alternative Name IKBKB (IKBKB Antibody Abstract)
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-203 AL708460.1 9-211 204-3077 AF080158.1 170-3043 3078-3916 AK023193.1 1980-2818
Alias Symbols: FLJ40509, IKK-beta, IKK2, IKKB, MGC131801, NFKBIKB
Protein Interaction Partner: BCL10, IKBKG, UBC, Snap23, RELA, NFKBIA, MTOR, RPTOR, ROCK1, IKBKB, CDC37, KEAP1, MAP3K7, FLNC, NGFR, EGLN3, SASH1, COPS5, COPS3, PPP1CA, TPX2, TNFRSF1A, AURKA, PRKCQ, MAP3K1, TFAP2C, NLRC5, PYCARD, TAB2, IKBKE, MAP3K14, CHUK, HSPB1, PRKCE, NAA20, TRAF3IP
Protein Size: 756
Molecular Weight 86 kDa
Gene ID 3551
NCBI Accession NM_001556, NP_001547
UniProt O14920
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-IKBKB Antibody Target Details IKBKB Handling ProductDetails: References for anti-IKBKB antibody (ABIN2779400) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 756 AA

Restrictions For Research Use only

Handling

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details ProductDetails: References for anti-IKBKB antibody (ABIN2779400) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-IKBKB antibody (ABIN2779400)

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling Images back to top
Background publications

Hu, Li, Mahavadi, Murthy: "Interleukin-1beta up-regulates RGS4 through the canonical IKK2/IkappaBalpha/NF-kappaB pathway in rabbit colonic smooth muscle." in: The Biochemical journal, Vol. 412, Issue 1, pp. 35-43, 2008

Images

Product Details anti-IKBKB Antibody Target Details IKBKB Application Details Handling ProductDetails: References for anti-IKBKB antibody (ABIN2779400) back to top
Supplier Images
Western Blotting (WB) image for anti-IKBKB antibody (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta) (Middle Region) (ABIN2779400) WB Suggested Anti-IKBKB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: