|Antigen||Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This IKBKB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-IKBKB AntibodyTarget Details IKBKB Application Details Handling Images
|Specificity||Anti-IKKb may react non-specifically with other proteins. Control peptide (R1141CP) will compete only with the specific reaction of antiserum with the IKKb subunit.|
|Characteristics||Concentration: 80 mg/mL (by Refractometry)|
|Purification||Prepared from monospecific antiserum by delipidation and defibrination.|
|Immunogen||IKKb peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
Target Details IKBKBProduct Details anti-IKBKB Antibody Application Details Handling Images back to top
|Alternative Name||IKBKB / IKKB (IKBKB Antibody Abstract)|
|Background||NFκB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFκB is negatively modulated by the inhibitory proteins IκB. In turn IκB is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-α and IKK-β which phosphorylate IκB leading to its degradation and the resulting translocation of NFκB to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-α, endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.Synonyms: I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-IKBKB Antibody Target Details IKBKB Handling Images back to top
Suitable for Immunoprecipitation,Western Blot and ELISA. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IKKb at a dilution of 1: 1000 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-IKKb is suitable for thedetection by immunoblot of human, mouse and rat IKKb showing an 87 kDa band.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IKBKB Antibody Target Details IKBKB Application Details Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.|