anti-Human IKBKG antibody for Intracellular Staining

Recommended IKBKG Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies
  • nemo
  • fj33c07
  • wu:fj33c07
  • zgc:113492
  • MGC97885
  • IKBKG
  • IKBKG_1
  • IKBKG_2
  • CG16910
  • DmIKK-gamma
  • DmIKKgamma
  • Dmel\\CG16910
  • Dmikkgamma
  • IKK
  • IKK-gamma
  • IKK[[gamma]]
  • IKKg
  • IKKgamma
  • KEY
  • Kenny
  • Key
  • dIKK
  • dIKK-gamma
  • dmIKKgamma
  • AMCBX1
  • FIP-3
  • FIP3
  • Fip3p
  • IP
  • IP1
  • IP2
  • IPD2
  • NEMO
  • ZC2HC9
  • Nemo
  • 1110037D23Rik
  • AI848108
  • AI851264
  • AW124339
  • IKK[g]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • kenny
  • inhibitor of kappaB kinase gamma
  • ikbkg
  • IKBKG
  • LOC782205
  • IKBKG_1
  • key
  • Ikbkg
Epitope
pSer376
37
32
18
13
12
10
9
9
9
7
7
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
331
115
82
10
8
6
6
5
5
4
3
3
2
2
2
1
Host
Mouse
242
97
3
2
2
Clonality
Monoclonal
Conjugate
This IKBKG antibody is un-conjugated
12
8
8
6
6
6
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Intracellular Staining (ICS), Western Blotting (WB)
264
102
74
60
51
40
25
21
6
5
5
3
2
1
1
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Catalog No. ABIN1177068
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177069 ICS PE Mouse IgG1, kappa pSer376 Log in to see N19-39 6

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies
Epitope pSer376
(37), (32), (18), (13), (12), (10), (9), (9), (9), (7), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(331), (115), (82), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1)
Host Mouse
(242), (97), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IKBKG antibody is un-conjugated
(12), (8), (8), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Intracellular Staining (ICS), Western Blotting (WB)
(264), (102), (74), (60), (51), (40), (25), (21), (6), (5), (5), (3), (2), (1), (1)
Pubmed 7 references available
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Product Details anti-IKBKG Antibody

Target Details IKBKG Application Details Handling References for anti-IKBKG antibody (ABIN1177068) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human IKKgamma Peptide
Clone N19-39
Isotype IgG1, kappa

Target Details IKBKG

Product Details anti-IKBKG Antibody Application Details Handling References for anti-IKBKG antibody (ABIN1177068) Images back to top
Antigen
Alternative Name IKKgamma (IKBKG Antibody Abstract)
Background The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKgamma (pS376). IKKgamma is also known an NEMO (NF-kappa-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. Activated NF-kappaB regulates genes involved in various pathways including inflammation, immunity, and cell survival signaling pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK)4 and MEKK1. IKKbeta has been shown to phosphorylate IKKgamma at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia virus type 1. IKKgamma has an essential regulatory role in the IKK complex and is required for the activation of the NF-kappaB pathway in response to multiple cellular stimuli. IKKgamma isoforms migrate as a doublet of ~50-55 kDa upon Western blot analysis.
Synonyms: IKBKG, IKKgamma, IKKG, IKK-gamma, AMCBX1, FIP3, IKKAP1, IP1, IP2, IPD2, NEMO
Molecular Weight ~50-55 kDa
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-IKBKG Antibody Target Details IKBKG Handling References for anti-IKBKG antibody (ABIN1177068) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-IKBKG Antibody Target Details IKBKG Application Details References for anti-IKBKG antibody (ABIN1177068) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IKBKG antibody (ABIN1177068)

Product Details anti-IKBKG Antibody Target Details IKBKG Application Details Handling Images back to top
Product cited in:

Shifera: "The zinc finger domain of IKKγ (NEMO) protein in health and disease." in: Journal of cellular and molecular medicine, Vol. 14, Issue 10, pp. 2404-14, 2010

Carter, Pennington, Ungurait, Ballard: "In vivo identification of inducible phosphoacceptors in the IKKgamma/NEMO subunit of human IkappaB kinase." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19642-8, 2003

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997