|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||18 Antibodies|
|Immunofluorescence (IF)||33 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||46 Antibodies|
|Immunohistochemistry (IHC)||62 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||42 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||189 Antibodies|
|Antigen||Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies|
|Conjugate||This IKBKG antibody is un-conjugated Alternatives|
Intracellular Staining (ICS), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-IKBKG AntibodyTarget Details IKBKG Application Details Handling References for anti-IKBKG Antibody (ABIN1177068) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Phosphorylated Human IKKgamma Peptide|
Target Details IKBKGProduct Details anti-IKBKG Antibody Application Details Handling References for anti-IKBKG Antibody (ABIN1177068) Images back to top
|Alternative Name||IKKgamma (IKBKG Antibody Abstract)|
The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKgamma (pS376). IKKgamma is also known an NEMO (NF-kappa-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. Activated NF-kappaB regulates genes involved in various pathways including inflammation, immunity, and cell survival signaling pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK)4 and MEKK1. IKKbeta has been shown to phosphorylate IKKgamma at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia virus type 1. IKKgamma has an essential regulatory role in the IKK complex and is required for the activation of the NF-kappaB pathway in response to multiple cellular stimuli. IKKgamma isoforms migrate as a doublet of ~50-55 kDa upon Western blot analysis.
Synonyms: IKBKG, IKKgamma, IKKG, IKK-gamma, AMCBX1, FIP3, IKKAP1, IP1, IP2, IPD2, NEMO
|Molecular Weight||~50-55 kDa|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-IKBKG Antibody Target Details IKBKG Handling References for anti-IKBKG Antibody (ABIN1177068) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-IKBKG Antibody Target Details IKBKG Application Details References for anti-IKBKG Antibody (ABIN1177068) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-IKBKG Antibody (ABIN1177068)Product Details anti-IKBKG Antibody Target Details IKBKG Application Details Handling Images back to top
|Product cited in:||
Shifera: "The zinc finger domain of IKKγ (NEMO) protein in health and disease." in: Journal of cellular and molecular medicine, Vol. 14, Issue 10, pp. 2404-14, 2010 (PubMed).
Carter, Pennington, Ungurait et al.: "In vivo identification of inducible phosphoacceptors in the IKKgamma/NEMO subunit of human IkappaB kinase." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19642-8, 2003 (PubMed).
Rothwarf, Zandi, Natoli et al.: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998 (PubMed).
Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998 (PubMed).
Nakano, Shindo, Sakon et al.: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998 (PubMed).
Mercurio, Zhu, Murray et al.: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997 (PubMed).
DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).