anti-Mouse (Murine) IKBKG antibody for Immunoprecipitation

Recommended IKBKG Antibody (supplied by: Log in to see )

Antigen
Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies
  • nemo
  • fj33c07
  • wu:fj33c07
  • zgc:113492
  • MGC97885
  • IKBKG
  • IKBKG_1
  • IKBKG_2
  • CG16910
  • DmIKK-gamma
  • DmIKKgamma
  • Dmel\\CG16910
  • Dmikkgamma
  • IKK
  • IKK-gamma
  • IKK[[gamma]]
  • IKKg
  • IKKgamma
  • KEY
  • Kenny
  • Key
  • dIKK
  • dIKK-gamma
  • dmIKKgamma
  • AMCBX1
  • FIP-3
  • FIP3
  • Fip3p
  • IP
  • IP1
  • IP2
  • IPD2
  • NEMO
  • ZC2HC9
  • Nemo
  • 1110037D23Rik
  • AI848108
  • AI851264
  • AW124339
  • IKK[g]
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
  • kenny
  • inhibitor of kappaB kinase gamma
  • ikbkg
  • IKBKG
  • LOC782205
  • IKBKG_1
  • key
  • Ikbkg
Reactivity
Human, Mouse (Murine)
329
112
81
10
8
6
6
5
5
4
3
3
2
2
2
1
Host
Mouse
240
97
2
2
2
Clonality
Monoclonal
Conjugate
This IKBKG antibody is un-conjugated
11
8
8
6
6
6
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
262
102
73
59
51
40
24
21
5
5
5
3
2
2
1
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Catalog No. ABIN967646
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN567775 IHC (p) IP WB Mouse IgG1 AA 262-319 Log in to see EA2-6 2
1 ABIN2745538 IP ELISA WB Rabbit AA 40-210 Log in to see Polyclonal
1 ABIN3198156 IC IF IHC IP WB Rabbit Log in to see Polyclonal
1 ABIN2565048 IHC (p) IP WB Mouse IgG1 AA 262-319 Log in to see EA2-6
1 ABIN2972911 IF/ICC IHC IP WB Rabbit Center Log in to see Polyclonal

General

Antigen Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies
Reactivity Human, Mouse (Murine)
(329), (112), (81), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1)
Host Mouse
(240), (97), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IKBKG antibody is un-conjugated
(11), (8), (8), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(262), (102), (73), (59), (51), (40), (24), (21), (5), (5), (5), (3), (2), (2), (1)
Pubmed 7 references available
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Product Details anti-IKBKG Antibody

Target Details IKBKG Application Details Handling ProductDetails: References for anti-IKBKG antibody (ABIN967646) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant Human IKKgamma
Clone C73
Isotype IgG1, kappa

Target Details IKBKG

Product Details anti-IKBKG Antibody Application Details Handling ProductDetails: References for anti-IKBKG antibody (ABIN967646) Images back to top
Antigen
Alternative Name IKK gamma (IKBKG Antibody Abstract)
Background The transcription factor NF-kappaB, (nuclear factor kappa-B) is controlled by interaction with an inhibitory subunit, IkappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IkappaB becomes degraded and NF-kappaB is released to the nucleus. The release of IkappaB from NF-kappaB is a critical step in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (aka IKK-1 and IKK-2), which phosphorylate critical residues of IkappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Thus the IKK kinases play an important role in the activation of NF-kappaB. IKKgamma migrates as a doublet between 50-55 kDa in SDS-PAGE.
Synonyms: NEMO
Molecular Weight 50-55 kDa doublet
Research Area Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades

Application Details

Product Details anti-IKBKG Antibody Target Details IKBKG Handling ProductDetails: References for anti-IKBKG antibody (ABIN967646) Images back to top
Application Notes Applications include western blot analysis and immunoprecipitation. In immunoprecipitation experiments, it is reported that the C73-764 antibody brings down the IKK complex (IKKalpha, IKKbeta, and IKKgamma). The immunoprecipitation application has not been evaluated. NIH/3T3 cells (ATCC CRL 1658) are recommended as a western blot positive control.
Comment

Related Products: ABIN968538, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-IKBKG Antibody Target Details IKBKG Application Details ProductDetails: References for anti-IKBKG antibody (ABIN967646) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-IKBKG antibody (ABIN967646)

Product Details anti-IKBKG Antibody Target Details IKBKG Application Details Handling Images back to top
Product cited in:

Chu, Ostertag, Li, Chang, Chen, Hu, Williams, Perrault, Karin: "JNK2 and IKKbeta are required for activating the innate response to viral infection." in: Immunity, Vol. 11, Issue 6, pp. 721-31, 2000

Li, Chu, Hu, Delhase, Deerinck, Ellisman, Johnson, Karin: "The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1839-45, 1999

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, 1998

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, 1998

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998

DiDonato, Hayakawa, Rothwarf, Zandi, Karin: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997

Mercurio, Zhu, Murray, Shevchenko, Bennett, Li, Young, Barbosa, Mann, Manning, Rao: "IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation." in: Science (New York, N.Y.), Vol. 278, Issue 5339, pp. 860-6, 1997

Images

Product Details anti-IKBKG Antibody Target Details IKBKG Application Details Handling ProductDetails: References for anti-IKBKG antibody (ABIN967646) back to top
Supplier Images
Western Blotting (WB) image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (ABIN967646) Western blot analysis of IKKgamma. Lysates from NIH/3T3 mouse embryonic fibroblast ce...
 image for anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (ABIN967646) anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) antibody (Image 2)