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|Antigen||Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies|
|Epitope||AA 278-396 Alternatives|
|Reactivity||Dog (Canine), Human, Rat (Rattus) Alternatives|
|Conjugate||This IKBKG antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-IKBKG AntibodyTarget Details IKBKG Application Details Handling ProductDetails: References for anti-IKBKG antibody (ABIN968464) Images
|Cross-Reactivity||Rat (Rattus), Human, Dog (Canine)|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse IKKgamma/NEMO aa. 278-396|
Target Details IKBKGProduct Details anti-IKBKG Antibody Application Details Handling ProductDetails: References for anti-IKBKG antibody (ABIN968464) Images back to top
|Alternative Name||IKK gamma (IKBKG Antibody Abstract)|
In most cells, NF-kappaB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkappaBalpha, IkappaBbeta, and IkappaBepsilon. Cell stimulation induces the release, activation, and nuclear translocation of NF-kappaB. Release of NF-kappaB results from the phosphorylation and subsequent proteolytic degradation of the IkappaB proteins. Two cytokine-inducible IkappaB kinases (IKKalpha and IKKbeta) phosphorylate and target the IkappaB proteins for degradation by the ubiquitin pathway. These kinases are components of a 700-900 kDa multisubunit complex that also contains NF-kappaB/RelA, IkappaBalpha, MEKK1, NIK, IKAP, and IKKgamma/NEMO (NF-kappaB essential modulator). IKKgamma contains two coiled-coil domains and a leucine zipper which allow it to form dimers and trimers that interact directly with IKKbeta. IKKgamma, essential for IKKalpha/IKKbeta activation of NF-kappaB, is located functionally and physically upstream of these subunits. In addition, IKKgamma enables the HTLV-1 Tax oncoprotein to interact with IKKbeta, resulting in constitutive activation of NF-kappaB and maintenance of cellular transformation. Thus, IKKgamma is an essential element of the IkappaB complex and an adaptor that mediates stable formation of Tax-IKK complexes.
|Molecular Weight||48 kDa|
|Research Area||Immunology, Innate Immunity, Atherosclerosis, Apoptosis/Necrosis, Transcription Factors|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades|
Application DetailsProduct Details anti-IKBKG Antibody Target Details IKBKG Handling ProductDetails: References for anti-IKBKG antibody (ABIN968464) Images back to top
Related Products: ABIN968547, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-IKBKG Antibody Target Details IKBKG Application Details ProductDetails: References for anti-IKBKG antibody (ABIN968464) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
ProductDetails: References for anti-IKBKG antibody (ABIN968464)Product Details anti-IKBKG Antibody Target Details IKBKG Application Details Handling Images back to top
|Product cited in:||
Weil, Schwamborn, Alcover, Bessia, Di Bartolo, Israël: "Induction of the NF-kappaB cascade by recruitment of the scaffold molecule NEMO to the T cell receptor." in: Immunity, Vol. 18, Issue 1, pp. 13-26, 2003
Chu, Shin, Yang, DiDonato, Ballard: "IKKgamma mediates the interaction of cellular IkappaB kinases with the tax transforming protein of human T cell leukemia virus type 1." in: The Journal of biological chemistry, Vol. 274, Issue 22, pp. 15297-300, 1999
Jin, Giordano, Kibler, Nakano, Jeang: "Role of adapter function in oncoprotein-mediated activation of NF-kappaB. Human T-cell leukemia virus type I Tax interacts directly with IkappaB kinase gamma." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17402-5, 1999
Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998
Yamaoka, Courtois, Bessia, Whiteside, Weil, Agou, Kirk, Kay, Israël: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998
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