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anti-Human IL1A antibody for Western Blotting

Recommended IL1A Antibody (supplied by: Log in to see )

Antigen
Interleukin 1 alpha (IL1A) Antibodies
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Epitope
N-Term
16
16
13
10
7
6
5
5
5
5
5
5
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine)
259
102
53
8
7
7
7
6
5
2
2
1
1
Host
Rabbit
223
138
34
10
7
7
7
3
3
Clonality
Polyclonal
Conjugate
This IL1A antibody is un-conjugated
68
31
15
14
8
8
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
277
275
65
63
52
35
22
21
20
19
17
10
9
5
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2785653
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN390844 WB Rabbit Ig AA 65-94, Ser87 Log in to see Polyclonal 1
0.13341247 ABIN652257 FACS IHC (p) WB Rabbit Ig AA 177-206, Center Log in to see Polyclonal 1
0.13341247 ABIN1724632 ELISA WB Mouse IgG2a Log in to see 3A6F11 2
0.13341247 ABIN1107769 EIA WB Mouse IgG2a Log in to see 1F3B3 2
0.13341247 ABIN453129 EIA WB Rabbit Ser87 Log in to see Polyclonal
0.13341247 ABIN1107770 EIA WB Mouse IgG2a Log in to see 1F3B3 2
0.13341247 ABIN223536 Neut WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1873200 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN151605 ELISA WB Mouse IgG2a Log in to see 1F3B3
0.13341247 ABIN634266 WB Rabbit N-Term Log in to see Polyclonal
0.13341247 ABIN2464070 ELISA WB Mouse IgG2a Log in to see 1F3B3
0.13341247 ABIN2467843 WB Chicken AA 1-271 Log in to see Polyclonal
0.13341247 ABIN3023109 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1583025 ELISA WB Mouse IgG2a Log in to see 1F3B3-3A6F11
0.13341247 ABIN1172163 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 113-271 Log in to see Polyclonal
0.13341247 ABIN932233 ELISA WB Mouse IgG2a Log in to see 1F3B3-3A6F11
0.13341247 ABIN561450 ELISA WB Mouse IgG1 kappa AA 172-271, partial Log in to see 4B8
0.13341247 ABIN516989 PLA WB Rabbit AA 1-271, full length Log in to see Polyclonal
0.13341247 ABIN2428275 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2405214 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL1A antibody for Western Blotting

Similar anti-IL1A Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine)
Western Blotting (WB) 170 Antibodies 53 Antibodies 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 20 Antibodies 16 Antibodies
Intracellular Staining (ICS) 2 Antibodies 3 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Immunoprecipitation (IP) 5 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies 8 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 49 Antibodies 13 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies 1 Antibodies

General

Antigen Interleukin 1 alpha (IL1A) Antibodies
Epitope N-Term
(16), (16), (13), (10), (7), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine)
(259), (102), (53), (8), (7), (7), (7), (6), (5), (2), (2), (1), (1)
Host Rabbit
(223), (138), (34), (10), (7), (7), (7), (3), (3)
Clonality
Polyclonal
Conjugate This IL1A antibody is un-conjugated
(68), (31), (15), (14), (8), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(277), (275), (65), (63), (52), (35), (22), (21), (20), (19), (17), (10), (9), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL1A Antibody

Target Details IL1A Application Details Handling References for anti-IL1A Antibody (ABIN2785653) Images
Blocking Peptide Blocking peptide for this product available: ABIN978484
Sequence VSYGPLHEGC MDQSVSLSIS ETSKTSKLTF KESMVVVATN GKVLKKRRLS
Predicted Reactivity Human : 100 %, Bovine : 90 %, Horse : 90 %, Dog : 80 %, Goat : 80 %, Sheep : 80 %
Characteristics This is a rabbit polyclonal antibody against IL1A. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1A

Target Details IL1A

Product Details anti-IL1A Antibody Application Details Handling References for anti-IL1A Antibody (ABIN2785653) Images back to top
Antigen
Alternative Name IL1A (IL1A Antibody Abstract)
Background The IL1A protein is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. The gene encoding IL1A and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
Molecular Weight 18 kDa
Gene ID 3552
NCBI Accession NM_000575, NP_000566
UniProt P01583
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

Product Details anti-IL1A Antibody Target Details IL1A Handling References for anti-IL1A Antibody (ABIN2785653) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 271 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1A Antibody Target Details IL1A Application Details References for anti-IL1A Antibody (ABIN2785653) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1A Antibody (ABIN2785653)

Product Details anti-IL1A Antibody Target Details IL1A Application Details Handling Images back to top
Product cited in:

Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-κB p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013

Zheng, Humphry, Maguire, Bennett, Clarke: "Intracellular interleukin-1 receptor 2 binding prevents cleavage and activity of interleukin-1α, controlling necrosis-induced sterile inflammation." in: Immunity, Vol. 38, Issue 2, pp. 285-95, 2013

Background publications

Ikeda, Sasazuki, Natsukawa, Shaura, Koizumi, Kasuga, Ohnami, Sakamoto, Yoshida, Iwasaki, Tsugane: "Screening of 214 single nucleotide polymorphisms in 44 candidate cancer susceptibility genes: a case-control study on gastric and colorectal cancers in the Japanese population." in: The American journal of gastroenterology, Vol. 103, Issue 6, pp. 1476-87, 2008

Images

Product Details anti-IL1A Antibody Target Details IL1A Application Details Handling References for anti-IL1A Antibody (ABIN2785653) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1A antibody (Interleukin 1 alpha) (N-Term) (ABIN2785653) WB Suggested Anti-IL1A Antibody Titration: 0.2-1 ug/ml
Positive Control:...