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anti-Mouse (Murine) IL1A antibody for Intracellular Staining

Recommended IL1A Antibody (supplied by: Log in to see )

Antigen
Interleukin 1 alpha (IL1A) Antibodies
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Reactivity
Mouse (Murine)
248
102
53
8
7
7
7
6
5
3
2
1
1
Host
Armenian Hamster
222
128
34
10
7
7
6
3
3
Clonality
Monoclonal
Conjugate
This IL1A antibody is conjugated to PE
68
31
14
14
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
276
271
61
55
51
33
21
20
20
18
16
10
9
4
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN279086
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008653603 ABIN2689744 ICS Neut ELISA WB Armenian Hamster IgG, lambda Log in to see ALF 3

Top referenced anti-IL1A antibody for Intracellular Staining

Similar anti-IL1A Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
ELISA 64 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 10 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Neutralization (Neut) 16 Antibodies
Western Blotting (WB) 53 Antibodies

General

Antigen Interleukin 1 alpha (IL1A) Antibodies
Reactivity Mouse (Murine)
(248), (102), (53), (8), (7), (7), (7), (6), (5), (3), (2), (1), (1)
Host Armenian Hamster
(222), (128), (34), (10), (7), (7), (6), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1A antibody is conjugated to PE
(68), (31), (14), (14), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(276), (271), (61), (55), (51), (33), (21), (20), (20), (18), (16), (10), (9), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1A Antibody

Target Details IL1A Application Details Handling References for anti-IL1A Antibody (ABIN279086) Images
Specificity The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23 % amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Characteristics The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23 % amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Synonyms: Synonyms: Interleukin-1 alpha, IL-1a, IL1a
Clone ALF-161
Isotype IgG

Target Details IL1A

Product Details anti-IL1A Antibody Application Details Handling References for anti-IL1A Antibody (ABIN279086) Images back to top
Antigen
Alternative Name IL-1a (IL1A Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

Product Details anti-IL1A Antibody Target Details IL1A Handling References for anti-IL1A Antibody (ABIN279086) Images back to top
Application Notes Applications reported: The ALF-161 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometry.

Applications tested: The fluorochrome-conjugated ALF-161 antibody is offered in 2 formats: - μg size: Can can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated can be used at test size: 5 μL (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test.
Comment

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions For Research Use only

Handling

Product Details anti-IL1A Antibody Target Details IL1A Application Details References for anti-IL1A Antibody (ABIN279086) Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C

References for anti-IL1A Antibody (ABIN279086)

Product Details anti-IL1A Antibody Target Details IL1A Application Details Handling Images back to top
Background publications

Rogers, Sheehan, Brunt, Dower, Unanue, Schreiber: "Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 3, pp. 1011-5, 1992

Images

Product Details anti-IL1A Antibody Target Details IL1A Application Details Handling References for anti-IL1A Antibody (ABIN279086) back to top
Supplier Images
 image for anti-IL1A antibody (Interleukin 1 alpha)  (PE) (ABIN279086) anti-Interleukin 1 alpha (IL1A) antibody (PE)
 image for anti-IL1A antibody (Interleukin 1 alpha)  (PE) (ABIN279086) anti-Interleukin 1 alpha (IL1A) antibody (PE) (Image 2)