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anti-Rat (Rattus) IL1A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended IL1A Antibody (supplied by: Log in to see )

Antigen
Interleukin 1 alpha (IL1A) Antibodies
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Reactivity
Rat (Rattus)
259
103
52
8
7
7
7
6
5
3
2
1
1
Host
Goat
224
137
33
10
7
7
7
3
3
Clonality
Polyclonal
Conjugate
This IL1A antibody is un-conjugated
68
31
15
14
8
8
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
276
274
65
63
52
34
22
20
19
19
17
10
9
5
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN116388
$ 392.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089690165 ABIN116387 EIA Func IHC (p) WB Goat Log in to see Polyclonal
0.00089690165 ABIN1385710 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00089690165 ABIN3201256 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
0.00089690165 ABIN1172170 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-IL1A Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 42 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Functional Studies (Func) 4 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Neutralization (Neut) 16 Antibodies
Western Blotting (WB) 45 Antibodies

General

Antigen Interleukin 1 alpha (IL1A) Antibodies
Reactivity Rat (Rattus)
(259), (103), (52), (8), (7), (7), (7), (6), (5), (3), (2), (1), (1)
Host Goat
(224), (137), (33), (10), (7), (7), (7), (3), (3)
Clonality
Polyclonal
Conjugate This IL1A antibody is un-conjugated
(68), (31), (15), (14), (8), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(276), (274), (65), (63), (52), (34), (22), (20), (19), (19), (17), (10), (9), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1A Antibody

Target Details IL1A Application Details Handling Images
Specificity This antibody reacts with Rat Interleukin 1 alpha
Purification Affinity chromatography
Immunogen Recombinant rat IL-1 alpha

Target Details IL1A

Product Details anti-IL1A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-1 alpha / IL-1A (IL1A Antibody Abstract)
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
Gene ID 24493
NCBI Accession NP_058715
UniProt P16598
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

Product Details anti-IL1A Antibody Target Details IL1A Handling Images back to top
Application Notes Immunohistochemistry: we recommend use of 1 μg per ml as a starting dilution. Paraffin sections may require pretreatment like Proteinase K or Trypsin predigestion toimprove staining. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rIL-1alpha (50 pg/mL), a concentration of 0.011-0.017 μg/mL of this antibody is required. ELISA: To detect rIL-1 alpha by direct ELISA (using 100 ml/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant rIL-1 alpha. Western blot: To detect rIL-1 alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rIL-1 alpha is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL1A Antibody Target Details IL1A Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.