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Mouse (Murine) IL1A ELISA Kit

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Antigen
Interleukin 1 alpha (IL1A) ELISA Kits
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
50
39
34
10
9
7
6
6
4
4
4
3
2
1
1
Methode Type
Sandwich ELISA
Detection Range
16-2000 pg/mL
Minimum Detection Limit
16 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN924814
$ 380.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN921060 Sandwich ELISA Cell Culture Supernatant, Serum 4.7-300 pg/mL 4.7 pg/mL Log in to see 8
0.32748017 ABIN415509 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see 1
0.32748017 ABIN1115631 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.63 pg/mL Log in to see
0.32748017 ABIN1888522 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 10-3000 pg/mL 10 pg/mL Log in to see
0.32748017 ABIN2708530 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32748017 ABIN2708529 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.32748017 ABIN2064507 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32748017 ABIN2762267 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 1.8-125 pg/mL 1.8-125 pg/mL Log in to see
0.32748017 ABIN2114187 Cell Culture Supernatant, Serum Log in to see
0.0012092679 ABIN1873500 Sandwich ELISA Serum, Plasma, Biological Fluids 0.78-50 pg/mL 0.78 pg/mL Log in to see
0.0012092679 ABIN365176 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant 1.56-100 pg/mL 1.56 pg/mL Log in to see
0.0012092679 ABIN3032809 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 12.3-3000 pg/mL 12.3 pg/mL Log in to see
0.0012092679 ABIN3198565 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012092679 ABIN625140 Serum, Plasma, Cell Culture Supernatant Log in to see
0.0012092679 ABIN1570664 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012092679 ABIN1112638 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.0012092679 ABIN2745457 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.6 pg/mL-1 ng/mL 15.6 pg/mL Log in to see
0.0012092679 ABIN2042510 Sandwich ELISA 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.0012092679 ABIN2114243 Cell Lysate, Serum, Plasma 16-2000 pg/mL 16 pg/mL Log in to see
0.0012092679 ABIN2792508 Cell Culture Supernatant, Serum, Plasma Log in to see

Top referenced IL1A ELISA Kit for ELISA

Similar IL1A ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 39 ELISA Kits

General

Antigen Interleukin 1 alpha (IL1A) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(50), (39), (34), (10), (9), (7), (6), (6), (4), (4), (4), (3), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 16-2000 pg/mL
Minimum Detection Limit 16 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details IL1A ELISA Kit

Target details Application Details Handling References for IL1A Kit (ABIN924814) Images
Purpose The OmniKine? Murine IL-1? ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine IL-1? concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine IL-1? while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non- specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Brand OmniKine™
Sample Type Cell Lysate, Serum, Plasma
Detection Method Colorimetric
Specificity The Murine IL-1? ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-1? proteins.
Cross-Reactivity (Details) The Murine IL-1α ELISA is capable of recognizing both recombinant and naturally produced Murine IL-1α proteins. The antigens listed below were tested at 50 ng/mL and exhibited less than 1% cross reactivity. Human: IL-1α Rat: IL-1α The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. Human: IL-1β, IL-18 Murine: IL-1β, IL-18 Rat: IL-1β
Characteristics The Murine IL-1alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IL-1alpha proteins within the range of 16-2000 pg/mL.
Components
  • Microstrips Coated w / Capture Antibody: 12 x 8-Well Microstrips
  • Protein Standard: Lyophilized (100 ng), Red container
  • Biotinylated Detection Antibody: Lyophilized, Yellow container
  • 400x Streptavidin-HRP: 30 μL, Blue container
  • Wash Buffer (10x): 50 mL, Clear containter
  • Assay Diluent: 50 mL, Clear container
  • Ready-to-Use Substrate: 12 mL, Brown container
  • Stop Solution: 12 mL, Clear container
  • Adhesive Plate Sealers: 4 Sheets
  • Technical Manual 1 Manual
Material not included The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
Microplate reader able to measure absorbance at 450 nm (with correction wavelength set to 540 nm or 570 nm)
Micropipettes with capability of measuring volumes ranging from 1 μl to 1 mL
Deionized or sterile water
Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
Graph paper or computer software capable of generating or displaying logarithmic functions
Absorbent paper or vacuum aspirator
Test tubes or microfuge tubes capable of storing ≥1 mL
Bench
top centrifuge (optional)
Bench
top vortex (optional)
Orbital shaker (optional)

Target details

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Antigen
Alternative Name IL-1alpha (IL1A ELISA Kit Abstract)
Background Murine IL-1α or Interleukin-1α is a 270 amino acid cytokine protein expressed from chromosome 2. Produced by activated macrophages, IL-1α stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins, typically monomeric and belonging to the IL-1 family, are involved in the inflammatory response as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Moreover, the lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Source: Entrez Gene: Il1a interleukin 1 alpha [Mus musculus], Swiss-Prot: P01582
Gene ID 16175
UniProt P01582
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

Product Details IL1A ELISA Kit Target details Handling References for IL1A Kit (ABIN924814) Images back to top
Plate Strips (12 x 8)
Protocol This particular immunoassay utilizes the quantitative technique of a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a sandwich format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IL-1alpha cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and sandwiching of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’- Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Sample Preparation If samples are to be used within 24 hours, aliquot and store at 4 °C. If samples are to be used over a long period of time, aliquot and store between -20 °C and -80 °C, depending on the duration of storage.
Note: Samples containing a visible precipitate or pellet must be clarified prior to use in the assay.
Caution: Avoid repeated freeze/thaw cycles to prevent loss of biological activity of proteins in experimental samples.
  • Cell Lysate and Supernatants:
    Remove large cell components via centrifugation and perform the assay. Cell lysates and supernatants require a dilution using Assay Diluent. A serial dilution may be performed to determine a suitable dilution factor for the sample. For future use of the sample, follow the sample storage guidelines stated above.
  • Serum:
    Allow samples to clot in a serum separator tube (SST) for 30 minutes. After sufficient clotting, centrifuge at 1000 x g for 15 minutes and remove serum from SST in preparation for the assay. Serum samples require at least a 1:50 dilution using Assay Diluent. For future use of the sample, follow the storage guidelines above.
  • Plasma:
    Use heparin, citrate or EDTA as an anticoagulant to gather plasma from original biological sample. After collection of the plasma, centrifuge for 15 minutes at 1000 x g. This step must be performed within 30 minutes of plasma collection. Plasma samples require at least a 1:50 dilution using Assay Diluent. Afterwards, perform the assay or for future use of the sample, follow the storage guidelines stated above.
Assay Procedure Note: If possible, all incubation steps should be performed on an orbital shaker to equilibrate solutions when added to the microplate wells. Also, all provided solutions should be at ambient temperature prior to use.
Note: Avoid adding solutions into wells at an angle, always keep pipette tip perpendicular to plate bottom.

Reconstitution of Provided Materials:
    1. Reconstitute the Biotin-Conjugated Detection Antibody in 67 µL of ddH₂O for a concentration of 180 µg/ml.
    2. Reconstitute the Protein Standard in 100 µL of ddH₂O for a concentration of 340 ng/ml.
    3. Dilute the 50 mL of 10x Wash Buffer in 450 mL of ddH2O for 500 mL of 1x Wash Buffer.
Addition of Known Standard and Unknown Sample to Immunoassay:
    The OmniKine™ Human CD163 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD163 proteins
Calculation of Results Generation of Standard Curve and Interpretation of Data
1. Average the duplicate or triplicate readings for each standard, control and sample and subtract the average zero standard optical density.
2. Generate a standard curve by using Microsoft Excel or other computer software capable of establishing a 4- Parameter Logistic (4-PL) curve fit. If using Excel or an alternative graphing tool, plot the average optical density values in absorbance units (y-axis) against the known standard concentrations in pg/ml (x-axis). Note: Only use the values in which a noticeable gradient can be established. Afterwards, generate a best fit curve or trend-line through the plotted points via regression analysis.
Restrictions For Research Use only

Handling

Product Details IL1A ELISA Kit Target details Application Details References for IL1A Kit (ABIN924814) Images back to top
Precaution of Use Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.
Handling Advice This ELISA kit is intended for research purposes only, NOT diagnostic or clinical procedures of any kind.
Materials included in this kit should NOT be used past the expiration date on the kit label.
Reagents or substrates included in this kit should NOT be mixed or substituted with reagents or substrates from any other kits.
Variations in pipetting technique, washing technique, operator laboratory technique, kit age, incubation time or temperature may cause differences in binding affinity of the materials provided.
The assay is designed to eliminate interference and background by other cellular macromolecules or factors present within any biological samples. However, the possibility of background noise cannot be fully excluded until all factors have been tested using the assay kit.

Reagents provided in this kit may be harmful if ingested, inhaled or absorbed through the skin. Please carefully review the MSDS for each reagent before conducting the experiment.
Stop Solution contains 2 N Sulfuric Acid (H2SO4) and is an extremely corrosive agent. Please wear proper eye, hand and face protection when handling this material. When the experiment is finished, be sure to rinse the plate with copious amounts of running water to dilute the Stop Solution prior to disposing the plate.
Storage 4 °C
Storage Comment Note: If used frequently, reagents may be stored at 4 °C.
  • Unopened Kits: Store at 4 °C for 6 months.
  • Microstrips Coated w/ Capture Antibody, 400x Streptavidin-HRP Wash Buffer (10x), Assay Diluent Ready-to-Use Substrate, Stop Solution: 6 Months at 4 °C
  • Protein Standard, Biotinylated Detection Antibody: Lyophilized: 6 Months (if Reconstituted: 1 Month) at 4 °C

References for IL1A Kit (ABIN924814)

Product Details IL1A ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Mateeva, Gangapuram, Mazzio, Eyunni, Soliman, Redda: "Biological evaluation of synthetic chalcone and flavone derivatives as anti-inflammatory agents." in: Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents, Vol. 24, Issue 4, pp. 1672-1680, 2015

Images

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Supplier Images
ELISA image for IL1A ELISA Kit (Interleukin 1 alpha) (ABIN924814) This is an example of what a typical standard curve will look like. You must make you...