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Rat (Rattus) IL1A ELISA Kit

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Antigen
Interleukin 1 alpha (IL1A) ELISA Kits
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Epitope
AA 115-270
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
50
40
33
10
9
7
6
6
4
4
4
3
2
1
1
Methode Type
Sandwich ELISA
Detection Range
4.69-300 pg/mL
Minimum Detection Limit
4.69 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411292
$ 350.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN1115632 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.63 pg/mL Log in to see 1
0.30628014 ABIN924835 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.30628014 ABIN416246 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 1
0.30628014 ABIN579022 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.30628014 ABIN1888523 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15-8000 pg/mL 15 pg/mL Log in to see
0.30628014 ABIN2708650 Sandwich ELISA Cell Culture Supernatant, Serum 4.69-300 pg/mL 4.69 pg/mL Log in to see
0.30628014 ABIN2762269 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 0.02-1.0 ng/mL 0.02-1.0 ng/mL Log in to see
0.30628014 ABIN2114186 Cell Culture Supernatant, Serum Log in to see
0.0012155735 ABIN365193 Sandwich ELISA Serum, Plasma, Tissue Homogenate 1.56-100 pg/mL 1.56 pg/mL Log in to see 2
0.0012155735 ABIN1873503 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012155735 ABIN625199 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 15-8000 pg/mL 15 pg/mL Log in to see
0.0012155735 ABIN3032871 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 10.9-8000 pg/mL 10.9 pg/mL Log in to see
0.0012155735 ABIN3199226 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012155735 ABIN1570666 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012155735 ABIN1112639 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.0012155735 ABIN2748827 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2042512 Sandwich ELISA 4.9-300 pg/mL 4.9 pg/mL Log in to see
0.0012155735 ABIN2114242 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.0012155735 ABIN2684629 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012155735 ABIN2684630 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 4.7-300 pg/mL 4.7 pg/mL Log in to see

Top referenced IL1A ELISA Kit for ELISA

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Application / Reactivity Rat (Rattus)
ELISA 33 ELISA Kits

General

Antigen Interleukin 1 alpha (IL1A) ELISA Kits
Epitope AA 115-270
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(50), (40), (33), (10), (9), (7), (6), (6), (4), (4), (4), (3), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 4.69-300 pg/mL
Minimum Detection Limit 4.69 pg/mL
Application ELISA
Pubmed 8 references available
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Product Details IL1A ELISA Kit

Target details Application Details Handling References for IL1A Kit (ABIN411292) Images
Purpose For quantitative detection of rat IL-1 alpha in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant rat IL-1 alpha
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <0.5pg/mL
Components
  • 96-well plate precoated with anti- rat IL-1 alpha antibod - 1
  • Lyophilized recombinant rat IL-1 alpha standar - 10ng/tubex2
  • Biotinylated anti- rat IL-1 alpha antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S115-S270

Target details

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Antigen
Alternative Name IL1A (IL1A ELISA Kit Abstract)
Background Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18 kDa.
Gene ID 24493
UniProt P16598
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1 alpha amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.69pg/mL rat IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IL-1 alpha standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 45, Standard deviation: 1.89, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 137, Standard deviation: 6.58, CV(%): 4.8
  • Sample 3: n=16, Mean(pg/ml): 211, Standard deviation: 10.76, CV(%): 5.1,
  • Sample 1: n=24, Mean(pg/ml): 49, Standard deviation: 2.646, CV(%): 5.4
  • Sample 2: n=24, Mean(pg/ml): 145, Standard deviation: 8.99, CV(%): 6.2
  • Sample 3: n=24, Mean(pg/ml): 230, Standard deviation: 15.41, CV(%): 6.7
Restrictions For Research Use only

Handling

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Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL1A Kit (ABIN411292)

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Product cited in:

Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, 2014

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, 2014

Liu, Yang, Guo, Zhu, Shi, Wang, Qi, Guo, Ye, Yang, Liang: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, 2013

Duan, Liao, Huang, Nie, Wu: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, 2012

Background publications

Diehl, Wang, Brooks, Burmeister, Califano, Wang, Schenkein: "Linkage disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis." in: Journal of periodontology, Vol. 70, Issue 4, pp. 418-30, 1999

Bailly, di Giovine, Blakemore, Duff: "Genetic polymorphism of human interleukin-1 alpha." in: European journal of immunology, Vol. 23, Issue 6, pp. 1240-5, 1993

Silver, Masson, George, Adam, Cox: "The IL-1 alpha and beta genes are closely linked (less than 70 kb) on mouse chromosome 2." in: Somatic cell and molecular genetics, Vol. 16, Issue 6, pp. 549-56, 1991

Modi, Masuda, Yamada, Oppenheim, Matsushima, OBrien: "Chromosomal localization of the human interleukin 1 alpha (IL-1 alpha) gene." in: Genomics, Vol. 2, Issue 4, pp. 310-4, 1989

Images

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Supplier Images
ELISA image for IL1A ELISA Kit (Interleukin 1 alpha) (ABIN411292) Rat IL-1 alpha PicoKine ELISA Kit standard curve