We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Rat (Rattus)|
|ELISA||33 ELISA Kits|
|Antigen||Interleukin 1 alpha (IL1A) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||4.69-300 pg/mL|
|Minimum Detection Limit||4.69 pg/mL|
|8 references available|
|Supplier||Log in to see|
Product Details IL1A ELISA KitTarget details Application Details Handling References for IL1A Kit (ABIN411292) Images
|Purpose||For quantitative detection of rat IL-1 alpha in cell culture supernates and serum.|
|Sample Type||Cell Culture Supernatant, Serum|
|Specificity||Natural and recombinant rat IL-1 alpha|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: E.coli
Immunogen sequence: S115-S270
Target detailsProduct Details IL1A ELISA Kit Application Details Handling References for IL1A Kit (ABIN411292) Images back to top
|Background||Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18 kDa.|
|Pathways||NF-kappaB Signaling, TCR Signaling|
Application DetailsProduct Details IL1A ELISA Kit Target details Handling References for IL1A Kit (ABIN411292) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||rat IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1 alpha amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.69pg/mL rat IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat IL-1 alpha standard solution and each sample is measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details IL1A ELISA Kit Target details Application Details References for IL1A Kit (ABIN411292) Images back to top
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for IL1A Kit (ABIN411292)Product Details IL1A ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, 2014 (PubMed).
Lin, Liu, Fang et al.: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, 2014 (PubMed).
Liu, Yang, Guo et al.: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, 2013 (PubMed).
Duan, Liao, Huang et al.: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, 2012 (PubMed).
Diehl, Wang, Brooks et al.: "Linkage disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis." in: Journal of periodontology, Vol. 70, Issue 4, pp. 418-30, 1999 (PubMed).
Bailly, di Giovine, Blakemore et al.: "Genetic polymorphism of human interleukin-1 alpha." in: European journal of immunology, Vol. 23, Issue 6, pp. 1240-5, 1993 (PubMed).
Modi, Masuda, Yamada et al.: "Chromosomal localization of the human interleukin 1 alpha (IL-1 alpha) gene." in: Genomics, Vol. 2, Issue 4, pp. 310-4, 1989 (PubMed).
Silver, Masson, George et al.: "The IL-1 alpha and beta genes are closely linked (less than 70 kb) on mouse chromosome 2." in: Somatic cell and molecular genetics, Vol. 16, Issue 6, pp. 549-56, 1991 (PubMed).
ImagesProduct Details IL1A ELISA Kit Target details Application Details Handling References for IL1A Kit (ABIN411292) back to top