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anti-Human IL1B antibody for ELISA (Detection)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Human
502
170
121
38
23
20
16
11
11
10
8
7
6
3
3
2
2
1
Host
Mouse
399
332
20
14
8
8
6
4
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Detection), Enzyme Immunoassay (EIA)
499
402
147
123
112
70
64
46
31
28
24
21
14
13
12
7
7
5
4
4
3
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN112300
$ 511.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000875046 ABIN191247 ELISA (Detection) ELISA Mouse IgM Log in to see 5C10 1
0.000875046 ABIN279961 ELISA (Detection) ELISpot Biotin Mouse IgG2b Log in to see CRM57

Top referenced anti-IL1B antibody for ELISA (Detection)

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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(502), (170), (121), (38), (23), (20), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (1)
Host Mouse
(399), (332), (20), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Detection), Enzyme Immunoassay (EIA)
(499), (402), (147), (123), (112), (70), (64), (46), (31), (28), (24), (21), (14), (13), (12), (7), (7), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity This antibody is specific for Human Interleukin-1 beta (IL-1b). Assay sensitivity is down to between 20-50 IU of IL-1 activity per ml. This antibody does not exhibit biological activity inhibition of Interleukin 1..
Purification Affinity Chromatography on Protein A
Immunogen Recombinant Interleukin-1 beta.
Clone 5C10
Isotype IgM

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 3553
NCBI Accession NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes ELISA: Catalogue numbers BM533 and BM534 have been used in a capture ELISA systemwhereby the first can be used to coat the plate and the second monoclonal used as theantibody-enzyme conjugate to complete the sandwich.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.