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anti-Human IL1B antibody for Flow Cytometry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
AA 148-174, Center
34
27
20
18
17
15
13
12
9
9
7
7
6
5
5
5
5
5
5
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
513
172
123
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Rabbit
400
342
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
502
412
153
125
115
70
66
47
36
29
25
24
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN652470
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3087334 ABIN2657949 FACS Alexa Fluor 647 Mouse IgG, IgG1 Log in to see JK1B-1 3
0.3087334 ABIN2662350 FACS Pacific Blue Mouse IgG1 kappa Log in to see H1b-98 2
0.3087334 ABIN2661941 FACS FITC Mouse IgG, IgG1 Log in to see JK1B-1 2
0.3087334 ABIN2661942 FACS FITC Mouse IgG1 kappa Log in to see H1b-98 2
0.3087334 ABIN1383939 FACS PE Mouse IgG1 Log in to see B-A15
0.3087334 ABIN263125 ICC FACS IF ELISA FITC Mouse IgG1 Log in to see JK1B-1
0.3087334 ABIN263124 ICC FACS IF ELISA Mouse IgG1 Log in to see JK1B-1
0.3087334 ABIN4324388 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 43N3D8 1
0.3087334 ABIN2487916 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.3087334 ABIN2680252 FACS ICS FITC Mouse IgG1 Log in to see CRM56
0.0010452945 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0010452945 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0010452945 ABIN2661219 FACS Biotin Mouse IgG1 kappa Log in to see H1b-98 2
0.0010452945 ABIN1107773 Func FACS Mouse IgG1 Log in to see B-A15 2
0.0010452945 ABIN2665129 FACS Mouse IgG, IgG1 Log in to see JK1B-1 2
0.0010452945 ABIN2660776 FACS Biotin Goat Log in to see Polyclonal 2
0.0010452945 ABIN1107774 FACS PE Mouse IgG1 Log in to see B-A15
0.0010452945 ABIN964777 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0010452945 ABIN1383938 FACS Mouse IgG1 Log in to see B-A15 2
0.0010452945 ABIN264345 FACS FITC Mouse IgG1 Log in to see AS10

Top referenced anti-IL1B antibody for Flow Cytometry

Similar anti-IL1B Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 221 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 95 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 148-174, Center
(34), (27), (20), (18), (17), (15), (13), (12), (9), (9), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(513), (172), (123), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(400), (342), (20), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(502), (412), (153), (125), (115), (70), (66), (47), (36), (29), (25), (24), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN652470) Images
Specificity This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the Center region of human IL1B.
Predicted Reactivity Monkey,Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 AA from the Central region of human IL1B.
Clone RB21917
Isotype Ig

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN652470) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Synonyms: Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2, IL1B
Molecular Weight 30748 DA
Gene ID 3553
UniProt P01584
Research Area Neurology
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN652470) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN652470) Images back to top
Format Liquid
Concentration 0.27 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-IL1B Antibody (ABIN652470)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Zhang, Liu, Huang, Su, Zhang, Wang, Li: "NF-?B activation and cell death after intracerebral hemorrhage in patients." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 35, Issue 7, pp. 1097-102, 2014

Liu, Tu, Chen, Wang, Hu, Dong: "Effects of Modified Simiao Decoction on IL-1 β and TNF α Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 406816, 2014

Background publications

Ito, Mukaiyama, Itoh, Nagase, Thogersen, Enghild, Sasaguri, Mori: "Degradation of interleukin 1beta by matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14657-60, 1996

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN652470) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (AA 148-174) (ABIN652470) Western blot analysis of IL1B Antibody (Center) (ABIN652470) in NCI-H460 cell line ly...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (AA 148-174) (ABIN652470) Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center)...
Flow Cytometry (FACS) image for anti-IL1B antibody (Interleukin 1, beta) (AA 148-174) (ABIN652470) Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histog...