anti-Human IL1B antibody for Flow Cytometry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
AA 148-174, Center
33
31
30
21
15
15
15
14
12
11
10
7
7
7
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
530
187
126
37
29
20
16
13
13
12
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
405
355
17
16
8
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
96
47
33
24
14
13
12
11
9
9
8
8
8
8
8
8
8
6
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
517
414
175
140
111
73
59
54
44
32
31
24
16
14
14
7
5
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN652470
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.327368 ABIN4895764 FACS Fluorescein Mouse IgG1 AA 117-269 Log in to see 8516 4
14.327368 ABIN4895765 FACS PE Mouse IgG1 AA 117-269 Log in to see 8516 4
14.327368 ABIN4324388 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 43N3D8 1
14.327368 ABIN2657949 FACS Alexa Fluor 647 Mouse IgG, IgG1 Log in to see JK1B-1 3
14.327368 ABIN2662350 FACS Pacific Blue Mouse IgG1 kappa Log in to see H1b-98 2
14.327368 ABIN2661941 FACS FITC Mouse IgG, IgG1 Log in to see JK1B-1 2
14.327368 ABIN4895760 FACS Allophycocyanin Rabbit IgG AA 117-269 Log in to see 1027B
14.327368 ABIN1383939 FACS PE Mouse IgG1 Log in to see B-A15
14.327368 ABIN2661942 FACS FITC Mouse IgG1 kappa Log in to see H1b-98 2
14.327368 ABIN4895761 FACS PE Rabbit IgG AA 117-269 Log in to see 1027B
14.327368 ABIN4895762 FACS Allophycocyanin Mouse IgG1 AA 117-269 Log in to see 8516
14.327368 ABIN4895763 FACS PerCP Mouse IgG1 AA 117-269 Log in to see 8516
14.327368 ABIN263125 ICC FACS IF ELISA FITC Mouse IgG1 Log in to see JK1B-1
14.327368 ABIN263124 ICC FACS IF ELISA Mouse IgG1 Log in to see JK1B-1
14.327368 ABIN3031434 FACS IHC ELISA WB Rabbit Ig Fraction AA 148-174 Log in to see Polyclonal
14.327368 ABIN3031433 FACS IHC ELISA WB Rabbit Ig Fraction AA 177-206 Log in to see Polyclonal
1 ABIN4899223 CyTOF FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
1 ABIN4899224 CyTOF FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
1 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
1 ABIN2661219 FACS Biotin Mouse IgG1 kappa Log in to see H1b-98 2

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 148-174, Center
(33), (31), (30), (21), (15), (15), (15), (14), (12), (11), (10), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(530), (187), (126), (37), (29), (20), (16), (13), (13), (12), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(405), (355), (17), (16), (8), (7), (6), (2), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(96), (47), (33), (24), (14), (13), (12), (11), (9), (9), (8), (8), (8), (8), (8), (8), (8), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(517), (414), (175), (140), (111), (73), (59), (54), (44), (32), (31), (24), (16), (14), (14), (7), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling ProductDetails: References for anti-IL1B antibody (ABIN652470) Images
Specificity This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the Center region of human IL1B.
Predicted Reactivity Monkey,Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 AA from the Central region of human IL1B.
Clone RB21917
Isotype Ig

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling ProductDetails: References for anti-IL1B antibody (ABIN652470) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Synonyms: Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2, IL1B
Molecular Weight 30748 DA
Gene ID 3553
UniProt P01584
Research Area Neurology
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling ProductDetails: References for anti-IL1B antibody (ABIN652470) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details ProductDetails: References for anti-IL1B antibody (ABIN652470) Images back to top
Format Liquid
Concentration 0.27 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-IL1B antibody (ABIN652470)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Zhang, Liu, Huang, Su, Zhang, Wang, Li: "NF-?B activation and cell death after intracerebral hemorrhage in patients." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 35, Issue 7, pp. 1097-102, 2014

Liu, Tu, Chen, Wang, Hu, Dong: "Effects of Modified Simiao Decoction on IL-1 β and TNF α Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 406816, 2014

Background publications

Ito, Mukaiyama, Itoh, Nagase, Thogersen, Enghild, Sasaguri, Mori: "Degradation of interleukin 1beta by matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14657-60, 1996

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling ProductDetails: References for anti-IL1B antibody (ABIN652470) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (AA 148-174), (Center) antibody (ABIN652470) Western blot analysis of IL1B Antibody (Center) (ABIN652470) in NCI-H460 cell line ly...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) (AA 148-174), (Center) antibody (ABIN652470) Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center)...
Flow Cytometry (FACS) image for anti-Interleukin 1, beta (IL1B) (AA 148-174), (Center) antibody (ABIN652470) Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histog...