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anti-Human IL1B antibody for Immunocytochemistry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
AA 117-269
30
26
24
23
18
16
15
12
11
10
9
7
7
6
6
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
532
179
128
39
31
22
18
17
12
11
8
7
7
4
3
2
2
2
1
1
Host
Mouse
409
350
17
16
8
8
6
3
2
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
100
48
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
539
434
176
125
110
73
59
58
37
29
25
24
14
14
13
7
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899224
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.12836 ABIN4899223 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
26.12836 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
1 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
1 ABIN1498871 ELISA ICC IF IHC (p) WB Mouse IgG1 Log in to see 3A6
1 ABIN263125 ICC FACS IF ELISA FITC Mouse IgG1 Log in to see JK1B-1
1 ABIN263124 ICC FACS IF ELISA Mouse IgG1 Log in to see JK1B-1
1 ABIN1859378 ICC IHC IP WB Rabbit IgG AA 117-269 Log in to see Polyclonal
1 ABIN4324371 ELISA ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 3A6
1 ABIN4880333 ELISA ICC IHC WB Mouse IgG1 Log in to see 3A6
1 ABIN256250 ICC FACS IF ELISA Mouse IgG2b kappa Log in to see H1b-27
1 ABIN345577 ICC ELISA WB Mouse IgG1 Log in to see 9627
1 ABIN1982142 ICC WB Sheep IgG AA 117-269 Log in to see Polyclonal
1 ABIN208673 Func ICC Neut Mouse IgG1 Log in to see
1 ABIN233195 Func ICC Neut FITC Mouse IgG1 Log in to see
1 ABIN645713 ICC IP IHC ELISA WB FITC Mouse IgG2b Log in to see

Top referenced anti-IL1B antibody for Immunocytochemistry

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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 117-269
(30), (26), (24), (23), (18), (16), (15), (12), (11), (10), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(532), (179), (128), (39), (31), (22), (18), (17), (12), (11), (8), (7), (7), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(409), (350), (17), (16), (8), (8), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(100), (48), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Neutralization (Neut), Western Blotting (WB)
(539), (434), (176), (125), (110), (73), (59), (58), (37), (29), (25), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN4899224) Images
Specificity Detects human IL-1 beta/IL-1F2 in Western blots. Shows less than 5 % cross-reactivity with recombinant mouse (rm) IL-1 beta and rpIL-1 beta and no cross-reactivity with rrIL-1 beta , rmIL-1 alpha , rhIL-1ra, rmIL-1ra, or rrIL-1 alpha .
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen E. coli-derived recombinant human IL-1 beta/IL-1F2, aa 117-269, Accession # P01584
Clone 8516
Isotype IgG1

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN4899224) Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN4899224) Images back to top
Application Notes Western Blot 1 μg/mL, Neutralization 0.001-0.003 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN4899224) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL1B Antibody (ABIN4899224)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Cheng, van Asten, Burns, Evans, Walter, Hashim, Hughes, Taams: "Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses." in: European journal of immunology, Vol. 46, Issue 9, pp. 2211-21, 2016 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Li, Hua, Qu, Yan, Ming, Jun, Yuan, Nan: "NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis." in: Archives of medical science : AMS, Vol. 12, Issue 5, pp. 950-958, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Oliver, Lovric, Yu, Christou, Aiken, Cooper, Walsh: "Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue." in: PLoS ONE, Vol. 10, Issue 8, pp. e0136514, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Alfaidi, Wilson, Daigneault, Burnett, Ridger, Chamberlain, Francis: "Neutrophil elastase promotes interleukin-1β secretion from human coronary endothelium." in: The Journal of biological chemistry, Vol. 290, Issue 40, pp. 24067-78, 2015 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Ansari, Dutta, Veettil, Dutta, Iqbal, Kumar, Roy, Chikoti, Singh, Chandran: "Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses." in: PLoS pathogens, Vol. 11, Issue 7, pp. e1005019, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Corvaisier, Delneste, Jeanvoine, Preisser, Blanchard, Garo, Hoppe, Barré, Audran, Bouvard, Saint-André, Jeannin: "IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation." in: PLoS biology, Vol. 10, Issue 9, pp. e1001395, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Zielinski, Mele, Aschenbrenner, Jarrossay, Ronchi, Gattorno, Monticelli, Lanzavecchia, Sallusto: "Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β." in: Nature, Vol. 484, Issue 7395, pp. 514-8, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Nicolaidou, Wong, Redpath, Ersek, Baban, Williams, Cope, Horwood: "Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation." in: PLoS ONE, Vol. 7, Issue 7, pp. e39871, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Aliahmadi, Gramlich, Grützkau, Hitzler, Krüger, Baumgrass, Schreiner, Wittig, Wanner, Peiser: "TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23." in: European journal of immunology, Vol. 39, Issue 5, pp. 1221-30, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Li, Yin, Yuan: "Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity." in: The Journal of cell biology, Vol. 181, Issue 2, pp. 321-33, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Acosta-Rodriguez, Napolitani, Lanzavecchia, Sallusto: "Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells." in: Nature immunology, Vol. 8, Issue 9, pp. 942-9, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

van Grevenstein, Hofland, van Rossen, van Koetsveld, Jeekel, van Eijck: "Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers." in: Digestive diseases and sciences, Vol. 52, Issue 10, pp. 2775-83, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Lee, Ho, Lin, Lin, Liu, Huang, Wang, Chung, Kuo: "Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway." in: American journal of respiratory cell and molecular biology, Vol. 35, Issue 4, pp. 407-14, 2006 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Banks, Farr, La Scola, Morley: "Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum." in: The Journal of pharmacology and experimental therapeutics, Vol. 299, Issue 2, pp. 536-41, 2001 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Yates, Burgess, Kocsis-Angle, Antal, Dority, Embury, Piotrkowski, Brunden: "Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia." in: Journal of neurochemistry, Vol. 74, Issue 3, pp. 1017-25, 2000 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN4899224) back to top
Supplier Images
Neutralization (Neut) image for anti-IL1B antibody (Interleukin 1, beta) (AA 117-269) (ABIN4899224) anti-Interleukin 1, beta (IL1B) (AA 117-269) antibody
Immunocytochemistry (ICC) image for anti-IL1B antibody (Interleukin 1, beta) (AA 117-269) (ABIN4899224) anti-Interleukin 1, beta (IL1B) (AA 117-269) antibody (Image 2)
Immunocytochemistry (ICC) image for anti-IL1B antibody (Interleukin 1, beta) (AA 117-269) (ABIN4899224) anti-Interleukin 1, beta (IL1B) (AA 117-269) antibody (Image 3)
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (AA 117-269) (ABIN4899224) anti-Interleukin 1, beta (IL1B) (AA 117-269) antibody (Image 4)