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anti-Human IL1B antibody for Immunocytochemistry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Reactivity
Human
529
168
123
39
26
21
17
12
12
10
8
7
6
3
3
2
2
2
1
1
Host
Mouse
409
350
17
16
8
8
6
3
2
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
100
48
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
505
414
164
126
105
71
60
58
39
29
27
24
14
13
7
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN969215
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.230143 ABIN4899224 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
26.230143 ABIN4899223 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
26.230143 ABIN1845349 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
1 ABIN1498871 ICC IF IHC ELISA WB Mouse IgG1 Log in to see 3A6
1 ABIN263125 ICC FACS IF ELISA FITC Mouse IgG1 Log in to see JK1B-1
1 ABIN263124 ICC FACS IF ELISA Mouse IgG1 Log in to see JK1B-1
1 ABIN1859378 ICC IHC IP WB Rabbit IgG AA 117-269 Log in to see Polyclonal
1 ABIN4324371 ELISA ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 3A6
1 ABIN4880333 ELISA ICC IHC WB Mouse IgG1 Log in to see 3A6
1 ABIN256250 ICC FACS IF ELISA Mouse IgG2b kappa Log in to see H1b-27
1 ABIN345577 ICC ELISA WB Mouse IgG1 Log in to see 9627
1 ABIN1982142 ICC WB Sheep IgG AA 117-269 Log in to see Polyclonal
1 ABIN208673 Func ICC Neut Mouse IgG1 Log in to see
1 ABIN233195 Func ICC Neut FITC Mouse IgG1 Log in to see
1 ABIN645713 ICC IP IHC ELISA WB FITC Mouse IgG2b Log in to see
1 ABIN3069447 ICC IF IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-IL1B antibody for Immunocytochemistry

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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(529), (168), (123), (39), (26), (21), (17), (12), (12), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(409), (350), (17), (16), (8), (8), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(100), (48), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(505), (414), (164), (126), (105), (71), (60), (58), (39), (29), (27), (24), (14), (13), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN969215) Images
Purification Ascites
Immunogen Purified recombinant fragment of human IL1B expressed in E. coli.
Clone 3A6
Isotype IgG1

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN969215) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Molecular Weight 31 kDa
Gene ID 3553
HGNC 3553
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN969215) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN969215) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-IL1B Antibody (ABIN969215)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Mohideen, Capili, Bilimoria, Yamada, Bonni, Lima: "A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9." in: Nature structural & molecular biology, Vol. 16, Issue 9, pp. 945-52, 2009

Kuo, Bentsi-Barnes, Barlow, Bae, Pisarska: "Sumoylation of forkhead L2 by Ubc9 is required for its activity as a transcriptional repressor of the Steroidogenic Acute Regulatory gene." in: Cellular signalling, Vol. 21, Issue 12, pp. 1935-44, 2009

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN969215) back to top
Supplier Images
ELISA image for anti-IL1B antibody (Interleukin 1, beta) (ABIN969215) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Immunocytochemistry (ICC) image for anti-IL1B antibody (Interleukin 1, beta) (ABIN969215) Figure 2: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (ABIN969215) Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (ABIN969215) Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (ABIN969215) Figure 5: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recom...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (ABIN969215) Figure 6: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc tra...