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|Antigen||Interleukin 1, beta (IL1B) Antibodies|
|Conjugate||This IL1B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
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Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling Images
|Specificity||Recognizes Human IL-1 beta. Does not recognize Mouse IL-1 beta, Human IL-1 alpha or a panel of other Human cytokines.|
|Characteristics||Synonyms: IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin|
|Purification||Protein G Chromatography|
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)|
|Background||Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2|
|Pathways||NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy|
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Neutralization: This antibody has been used at a 100-fold molar excess to neutralize >/=95 % of the biological activity of human IL-1b. Immunohistochemistry on frozen sections: This antibody has been used at 1-10 μg/mL tostain human IL-1b expressed in mononuclear cells, following fixation with 4 %Paraffin-Formaldehyde in PBS for 10 minutes at 4 °C, plus 0.1 % Saponin 20 minutes at RT. Immunoprecipitation: This antibody has been used to immunoprecipitate >90 % hIL-1b insolution using a double-antibody method. (Midgley, AR and Hepburn, MR, MethodsEnzymol. , 70, 266, 1980). ELISA: This antibody has been used as a solution phase antibody at 2 μg/mL in a two siteELISA with biotin conjugated IL-1beta antibody (ABIN264349). Typically the ELISA has aworking range of 0-500 pg/mL and a sensitivity of 5 pg/mL with the appropriate standard. Recommended sample volumes are 25-200 μL. Recovery of hIL-1b in human serum istypically >85 % in the ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
|Buffer||PBS, 0.08 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|