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anti-Human IL1B antibody for Immunohistochemistry (Frozen Sections)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Reactivity
Human
510
177
125
37
27
20
16
13
13
12
8
7
6
4
4
2
2
2
1
1
Host
Mouse
385
342
17
16
8
7
6
3
2
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
94
45
33
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
508
406
165
124
110
72
58
52
38
28
24
23
14
14
13
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN264346
$ 445.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116862 EIA Func IHC (fro) IHC (p) IP RIA WB Rabbit Log in to see Polyclonal 3
1 ABIN116861 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal 2
1 ABIN799612 IHC (fro) IHC (p) IP RIA ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN261277 IHC (fro) IHC (p) IHC ELISA Mouse IgG2b Log in to see 11E5
1 ABIN268027 IHC (fro) IHC (p) IHC ELISA Mouse IgG2a Log in to see 6E10
1 ABIN4224529 ELISA IHC (fro) IHC (p) Alexa Fluor 405 Mouse IgG2b Log in to see 11E5
1 ABIN4224530 ELISA IHC (fro) IHC (p) Alexa Fluor 488 Mouse IgG2b Log in to see 11E5
1 ABIN4224532 ELISA IHC (fro) IHC (p) Alexa Fluor 700 Mouse IgG2b Log in to see 11E5
1 ABIN4224531 ELISA IHC (fro) IHC (p) Alexa Fluor 647 Mouse IgG2b Log in to see 11E5
1 ABIN4224537 ELISA IHC (fro) IHC (p) DyLight 680 Mouse IgG2b Log in to see 11E5
1 ABIN4224538 ELISA IHC (fro) IHC (p) DyLight 488 Mouse IgG2b Log in to see 11E5
1 ABIN4224540 ELISA IHC (fro) IHC (p) DyLight 755 Mouse IgG2b Log in to see 11E5
1 ABIN4224541 ELISA IHC (fro) IHC (p) PerCP Mouse IgG2b Log in to see 11E5
1 ABIN4224558 ELISA IHC (fro) IHC (p) DyLight 755 Mouse IgG2a Log in to see 6E10
1 ABIN4224562 ELISA IHC (fro) IHC (p) DyLight 350 Mouse IgG2a Log in to see 6E10
1 ABIN4224550 ELISA IHC (fro) IHC (p) Alexa Fluor 700 Mouse IgG2a Log in to see 6E10
1 ABIN4224547 ELISA IHC (fro) IHC (p) Alexa Fluor 405 Mouse IgG2a Log in to see 6E10
1 ABIN4224548 ELISA IHC (fro) IHC (p) Alexa Fluor 488 Mouse IgG2a Log in to see 6E10
1 ABIN4224543 ELISA IHC (fro) IHC (p) DyLight 550 Mouse IgG2b Log in to see 11E5
1 ABIN4224544 ELISA IHC (fro) IHC (p) DyLight 350 Mouse IgG2b Log in to see 11E5

Top referenced anti-IL1B antibody for Immunohistochemistry (Frozen Sections)

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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(510), (177), (125), (37), (27), (20), (16), (13), (13), (12), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Mouse
(385), (342), (17), (16), (8), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(94), (45), (33), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(508), (406), (165), (124), (110), (72), (58), (52), (38), (28), (24), (23), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity Recognizes Human IL-1 beta. Does not recognize Mouse IL-1 beta, Human IL-1 alpha or a panel of other Human cytokines.
Characteristics Synonyms: IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin
Purification Protein G Chromatography
Immunogen Genename: IL1B
Clone AS10
Isotype IgG1

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 3553
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Neutralization: This antibody has been used at a 100-fold molar excess to neutralize >/=95 % of the biological activity of human IL-1b. Immunohistochemistry on frozen sections: This antibody has been used at 1-10 μg/mL tostain human IL-1b expressed in mononuclear cells, following fixation with 4 %Paraffin-Formaldehyde in PBS for 10 minutes at 4 °C, plus 0.1 % Saponin 20 minutes at RT. Immunoprecipitation: This antibody has been used to immunoprecipitate >90 % hIL-1b insolution using a double-antibody method. (Midgley, AR and Hepburn, MR, MethodsEnzymol. , 70, 266, 1980). ELISA: This antibody has been used as a solution phase antibody at 2 μg/mL in a two siteELISA with biotin conjugated IL-1beta antibody (ABIN264349). Typically the ELISA has aworking range of 0-500 pg/mL and a sensitivity of 5 pg/mL with the appropriate standard. Recommended sample volumes are 25-200 μL. Recovery of hIL-1b in human serum istypically >85 % in the ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.08 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months