anti-Human IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
30
29
25
20
15
15
15
14
12
11
11
7
7
7
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
517
178
125
37
28
20
16
13
12
11
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
387
351
17
16
8
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
95
45
33
22
14
12
11
11
9
9
8
8
8
8
8
8
8
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
508
407
164
132
111
72
59
53
38
30
24
24
14
14
13
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN5326748
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.054504 ABIN2785655 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.054504 ABIN652470 FACS IHC (p) WB Rabbit Ig AA 148-174, Center Log in to see Polyclonal 3
3.054504 ABIN453702 EIA WB Rabbit Middle Region Log in to see Polyclonal 1
3.054504 ABIN4899223 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
3.054504 ABIN4899224 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
3.054504 ABIN4324388 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 43N3D8 1
3.054504 ABIN446969 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal 3
3.054504 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
3.054504 ABIN4324384 WB Mouse IgG1 kappa Log in to see 43N1G6 1
3.054504 ABIN223537 Neut WB Rabbit IgG Log in to see Polyclonal 2
3.054504 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
3.054504 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
3.054504 ABIN3042625 WB Rabbit IgG AA 249-269, C-Term Log in to see Polyclonal
3.054504 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
3.054504 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.054504 ABIN3187737 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.054504 ABIN4324362 ELISA WB Rabbit N-Term Log in to see Polyclonal 1
3.054504 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
3.054504 ABIN2706371 IHC WB Rabbit Center Log in to see Polyclonal
3.054504 ABIN516996 IF IP ELISA WB Mouse IgG2b kappa AA 170-269, partial Log in to see 2A8 1

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Human Mouse (Murine) Rabbit Dog (Canine) Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 304 Antibodies 127 Antibodies 27 Antibodies 12 Antibodies 12 Antibodies
Radioimmunoassay (RIA) 9 Antibodies 4 Antibodies 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 29 Antibodies 32 Antibodies 4 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(30), (29), (25), (20), (15), (15), (15), (14), (12), (11), (11), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
(517), (178), (125), (37), (28), (20), (16), (13), (12), (11), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(387), (351), (17), (16), (8), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(95), (45), (33), (22), (14), (12), (11), (11), (9), (9), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(508), (407), (164), (132), (111), (72), (59), (53), (38), (30), (24), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling ProductDetails: References for anti-IL1B antibody (ABIN5326748) Images
Blocking Peptide Blocking peptide for this product available: ABIN978485
Sequence MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL
Predicted Reactivity Dog: 92%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 92%
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling ProductDetails: References for anti-IL1B antibody (ABIN5326748) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Alias Symbols: IL-1, IL1-BETA, IL1F2
Protein Interaction Partner: PTPN1, LYN, FYN, CMA1, CASP1, APP, SP1, EGR1, ELAVL1, CASP4, IL1RAP, PRTN3, IL1R2, IL1R1, IL1B, MMP2, ADRB2, A2M, UBE2N, ZNF675, MAPK8IP2,
Protein Size: 269
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling ProductDetails: References for anti-IL1B antibody (ABIN5326748) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 269 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details ProductDetails: References for anti-IL1B antibody (ABIN5326748) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-IL1B antibody (ABIN5326748)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-κB p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling ProductDetails: References for anti-IL1B antibody (ABIN5326748) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN5326748) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN5326748) Sample Type: Human Macrophange Cells
Green: primary
Red: p...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN5326748) WB Suggested Anti-IL1B
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500