You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
AA 148-174, Center
30
26
25
23
18
15
15
12
11
9
9
7
7
6
6
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
532
179
128
39
31
22
18
17
12
11
8
7
7
4
3
2
2
2
1
1
Host
Rabbit
408
351
17
16
8
8
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
48
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
539
434
176
125
109
74
59
58
38
29
25
24
14
14
13
7
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN652470
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN2785655 IHC WB Rabbit N-Term Log in to see Polyclonal 1
2.9590726 ABIN5326748 IHC WB Rabbit N-Term Log in to see Polyclonal 1
2.9590726 ABIN453702 EIA WB Rabbit Middle Region Log in to see Polyclonal 1
2.9590726 ABIN4899224 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
2.9590726 ABIN4899223 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
2.9590726 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
2.9590726 ABIN223537 Neut WB Rabbit IgG Log in to see Polyclonal 2
2.9590726 ABIN4324388 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 43N3D8 1
2.9590726 ABIN446969 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal 3
2.9590726 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
2.9590726 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
2.9590726 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
2.9590726 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9590726 ABIN3187737 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9590726 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
2.9590726 ABIN1107772 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3A6 2
2.9590726 ABIN3042625 WB Rabbit IgG AA 249-269, C-Term Log in to see Polyclonal
2.9590726 ABIN2706371 IHC WB Rabbit Center Log in to see Polyclonal
2.9590726 ABIN516996 IF IP ELISA WB Mouse IgG2b kappa AA 170-269, partial Log in to see 2A8 1
2.9590726 ABIN1537701 WB Mouse IgG1 AA 148-174, Center Log in to see 614CT4-3-1

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Human
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 236 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 99 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 148-174, Center
(30), (26), (25), (23), (18), (15), (15), (12), (11), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(532), (179), (128), (39), (31), (22), (18), (17), (12), (11), (8), (7), (7), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(408), (351), (17), (16), (8), (8), (6), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(100), (48), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(539), (434), (176), (125), (109), (74), (59), (58), (38), (29), (25), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN652470) Images
Specificity This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the Center region of human IL1B.
Predicted Reactivity Monkey,Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 AA from the Central region of human IL1B.
Clone RB21917
Isotype Ig

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN652470) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Synonyms: Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2, IL1B
Molecular Weight 30748 DA
Gene ID 3553
UniProt P01584
Research Area Neurology
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN652470) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN652470) Images back to top
Format Liquid
Concentration 0.27 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-IL1B Antibody (ABIN652470)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Zhang, Liu, Huang, Su, Zhang, Wang, Li: "NF-?B activation and cell death after intracerebral hemorrhage in patients." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, Vol. 35, Issue 7, pp. 1097-102, 2014

Liu, Tu, Chen, Wang, Hu, Dong: "Effects of Modified Simiao Decoction on IL-1 β and TNF α Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 406816, 2014

Background publications

Ito, Mukaiyama, Itoh, Nagase, Thogersen, Enghild, Sasaguri, Mori: "Degradation of interleukin 1beta by matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14657-60, 1996

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN652470) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (AA 148-174) (ABIN652470) Western blot analysis of IL1B Antibody (Center) (ABIN652470) in NCI-H460 cell line ly...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (AA 148-174) (ABIN652470) Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center)...
Flow Cytometry (FACS) image for anti-IL1B antibody (Interleukin 1, beta) (AA 148-174) (ABIN652470) Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histog...