You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
N-Term
33
26
20
18
17
16
13
12
9
9
7
7
6
6
5
5
5
5
5
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
511
171
122
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Rabbit
399
341
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
500
411
150
126
115
70
66
47
35
29
25
24
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2785655
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2785654 IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.13341247 ABIN652470 FACS IHC (p) WB Rabbit Ig AA 148-174, Center Log in to see Polyclonal 3
0.13341247 ABIN453702 EIA WB Rabbit Middle Region Log in to see Polyclonal 1
0.13341247 ABIN223537 Neut WB Rabbit IgG Log in to see Polyclonal 2
0.13341247 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
0.13341247 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.13341247 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
0.13341247 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.13341247 ABIN1107772 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3A6 2
0.13341247 ABIN516996 IF IP ELISA WB Mouse IgG2b kappa AA 170-269, partial Log in to see 2A8 1
0.13341247 ABIN2706371 IHC WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN1537701 WB Mouse IgG1 AA 148-174, Center Log in to see 614CT4-3-1
0.13341247 ABIN3042625 WB Rabbit IgG AA 249-269, C-Term Log in to see Polyclonal
0.13341247 ABIN516995 PLA WB Rabbit AA 1-269, full length Log in to see Polyclonal 1
0.13341247 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit IgG N-Term Log in to see Polyclonal 9
0.13341247 ABIN103012 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Cleavage Site, N-Term, AA 153 Log in to see Polyclonal
0.13341247 ABIN189446 WB Rabbit IgG Log in to see EP407Y
0.13341247 ABIN189447 WB Rabbit IgG Log in to see EP408Y
0.13341247 ABIN498526 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN1873201 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 222 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 97 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 121 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(33), (26), (20), (18), (17), (16), (13), (12), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(511), (171), (122), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(399), (341), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(500), (411), (150), (126), (115), (70), (66), (47), (35), (29), (25), (24), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785655) Images
Blocking Peptide Blocking peptide for this product available: ABIN978486
Sequence DLDLCPLDGG IQLRISDHHY SKGFRQAASV VVAMDKLRKM LVPCPQTFQE
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN2785655) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN2785655) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 153 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN2785655) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN2785655)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Piccini, Carta, Tassi, Lasiglié, Fossati, Rubartelli: "ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1beta and IL-18 secretion in an autocrine way." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8067-72, 2008

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785655) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785655) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785655) Sample Type: Human Macrophange Cells
Green: primary
Red: p...