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anti-Human IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
30
29
26
20
15
15
15
14
12
11
10
7
7
7
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
510
177
125
37
27
20
16
13
13
12
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
384
343
17
16
8
7
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
94
45
33
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
507
406
164
124
110
72
59
53
38
29
24
24
14
14
13
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN2785655
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0922565 ABIN5326748 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.0922565 ABIN652470 FACS IHC (p) WB Rabbit Ig AA 148-174, Center Log in to see Polyclonal 3
3.0922565 ABIN453702 EIA WB Rabbit Middle Region Log in to see Polyclonal 1
3.0922565 ABIN4899224 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
3.0922565 ABIN4899223 FACS ICC Neut WB Mouse IgG1 AA 117-269 Log in to see 8516 15
3.0922565 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
3.0922565 ABIN223537 Neut WB Rabbit IgG Log in to see Polyclonal 2
3.0922565 ABIN446969 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal 3
3.0922565 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
3.0922565 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
3.0922565 ABIN4324388 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 43N3D8 1
3.0922565 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
3.0922565 ABIN3042625 WB Rabbit IgG AA 249-269, C-Term Log in to see Polyclonal
3.0922565 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0922565 ABIN3187737 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0922565 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
3.0922565 ABIN1107772 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3A6 2
3.0922565 ABIN2706371 IHC WB Rabbit Center Log in to see Polyclonal
3.0922565 ABIN516996 IF IP ELISA WB Mouse IgG2b kappa AA 170-269, partial Log in to see 2A8 1
3.0922565 ABIN1537701 WB Mouse IgG1 AA 148-174, Center Log in to see 614CT4-3-1

Top referenced anti-IL1B antibody for Western Blotting

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Application / Reactivity Human
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Cell Culture (CC) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(30), (29), (26), (20), (15), (15), (15), (14), (12), (11), (10), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(510), (177), (125), (37), (27), (20), (16), (13), (13), (12), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(384), (343), (17), (16), (8), (7), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(94), (45), (33), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(507), (406), (164), (124), (110), (72), (59), (53), (38), (29), (24), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785655) Images
Blocking Peptide Blocking peptide for this product available: ABIN978486
Sequence DLDLCPLDGG IQLRISDHHY SKGFRQAASV VVAMDKLRKM LVPCPQTFQE
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN2785655) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: IL-1, IL1-BETA, IL1F2
Protein Interaction Partner: PTPN1, LYN, FYN, CMA1, CASP1, APP, SP1, EGR1, ELAVL1, CASP4, IL1RAP, PRTN3, IL1R2, IL1R1, IL1B, MMP2, ADRB2, A2M, UBE2N, ZNF675, MAPK8IP2,
Protein Size: 153
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN2785655) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 153 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN2785655) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN2785655)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Piccini, Carta, Tassi, Lasiglié, Fossati, Rubartelli: "ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1beta and IL-18 secretion in an autocrine way." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 23, pp. 8067-72, 2008

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN2785655) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785655) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN2785655) Sample Type: Human Macrophange Cells
Green: primary
Red: p...