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anti-Monkey IL1B antibody for Radioimmunoassay

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Human, Monkey
476
166
117
38
20
20
16
11
10
8
8
7
5
3
3
2
2
1
Host
Rabbit
390
312
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
98
49
33
21
12
10
9
9
8
8
8
8
8
8
8
8
7
7
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
478
392
138
110
101
68
63
44
29
28
23
18
14
12
12
7
5
5
4
4
4
3
3
1
1
1
1
1
1
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Catalog No. ABIN116862
$ 1,435.50
Plus shipping costs $45.00

Top referenced anti-IL1B antibody for Radioimmunoassay

Similar anti-IL1B Antibodies

Application / Reactivity Monkey Human
ELISA 1 Antibodies 215 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 15 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 5 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human, Monkey
(476), (166), (117), (38), (20), (20), (16), (11), (10), (8), (8), (7), (5), (3), (3), (2), (2), (1)
Host Rabbit
(390), (312), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(98), (49), (33), (21), (12), (10), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (7), (7), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(478), (392), (138), (110), (101), (68), (63), (44), (29), (28), (23), (18), (14), (12), (12), (7), (5), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN116862) Images
Specificity This antibody is primarily directed against mature, 17,000 MW human IL1 beta and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1b in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW IL-1b containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1b precursor molecule. Denatured 31,000 precursor IL-1b will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1b exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, a 1:100 to 1:200 dilution of this antiserum detected the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1b precursor in lysates. This antibody is also useful for neutralization of human and primate IL-1b activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1b . It will neutralize primate IL-1b. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically.
Purification DEAE fractionation.
Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant 153 aa human IL1 beta produced in E.coli. Remarks: The MW of the recombinant IL-1b was 17,000 with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL1 beta converting enzyme (ICE, capase-1).

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN116862) Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 3553
NCBI Accession NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN116862) Images back to top
Application Notes This IgG fraction of anti-Human IL1 beta antibody has been tested for use inNeutralizations, ELISA, Radioimmunoassays, Flow cytometry, Immunohistochemistry,Immunoblotting and Immunoprecipitation. It recognizes the 17,000 MW mature IL1 beta. Recommended Dilutions: Neutralization: 1/100Radioimmunoassay: 1/5,000-1/10,000Western blot: 1/1,000, in Immunoblots, typically, IL1 beta is detected from supernatants orlysates of 2 x 10^6 endotoxin-stimulated human peripheral blood mononuclear cells(PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coliLPS. Immunoprecipitation: 1/400-1/800, Pre- clearing the preparation with a non-specific rabbitIgG to reduce background. Immunohistochemistry: 1/100-1/200, either Paraffin fixation or cryofixation can be used for
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN116862) Images back to top
Concentration 2.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 without preservatives or stabilizers.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL1B Antibody (ABIN116862)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Herzyk, Berger, Allen, Wewers: "Sandwich ELISA formats designed to detect 17 kDa IL-1 beta significantly underestimate 35 kDa IL-1 beta." in: Journal of immunological methods, Vol. 148, Issue 1-2, pp. 243-54, 1992

Auron, Webb, Rosenwasser, Mucci, Rich, Wolff, Dinarello: "Nucleotide sequence of human monocyte interleukin 1 precursor cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 24, pp. 7907-11, 1985

Van Damme, De Ley, Opdenakker, Billiau, De Somer, Van Beeumen: "Homogeneous interferon-inducing 22K factor is related to endogenous pyrogen and interleukin-1." in: Nature, Vol. 314, Issue 6008, pp. 266-8, 1985