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anti-Mouse (Murine) IL1B antibody for ELISA (Capture)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Mouse (Murine), Rat (Rattus)
503
169
120
38
23
20
16
11
11
10
8
7
6
3
3
2
2
1
Host
Armenian Hamster
399
333
20
14
8
8
6
3
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture), Neutralization (Neut)
499
402
147
123
112
69
64
46
31
29
24
21
14
13
12
7
6
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN282499
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN281601 ELISA (Capture) Neut Armenian Hamster IgG Log in to see B122

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 2 Antibodies
ELISA 60 Antibodies 83 Antibodies
ELISA (Capture) 2 Antibodies 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Neutralization (Neut) 16 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 90 Antibodies
ELISA (Detection) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(503), (169), (120), (38), (23), (20), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (1)
Host Armenian Hamster
(399), (333), (20), (14), (8), (8), (6), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), Neutralization (Neut)
(499), (402), (147), (123), (112), (69), (64), (46), (31), (29), (24), (21), (14), (13), (12), (7), (6), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity The B122 antibody reacts with mouse and rat interleukin-1β (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.
Characteristics The B122 antibody reacts with mouse and rat interleukin-1β (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T- helper cells, inducing IL-2 secretion and expression of IL-2 receptors.
Synonyms: Synonyms: Interleukin-1 beta, IL-1b, IL1b il-1beta
Purification Affinity Purified
Endotoxin Level <0.001 ng endotoxin/μg
Grade Functional Grade
Clone B122
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-1b (IL1B Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Applications reported: The B122 antibody has been reported for use in cytokine neutralization and ELISA capture.

Applications tested: The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1 beta bioactivity. The B122 antibody at 0.125 μg/mL has been found to inhibit by 50 % the biological effects of 4.0 ng/mL mouse IL-1 beta in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1 beta) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 1 mg/mL
Buffer aqueous buffer, no sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Use in sterile environment.
Storage 4 °C