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anti-Mouse (Murine) IL1B antibody for Flow Cytometry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Mouse (Murine)
513
171
123
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Rat
401
341
19
14
8
8
6
4
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is conjugated to EFluor 450
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
502
411
152
125
116
70
66
47
35
29
25
24
13
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN2680266
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30636168 ABIN2680255 FACS ICS FITC Rat IgG1 kappa Log in to see NJTEN3
0.30636168 ABIN2680258 FACS APC Rat IgG1 kappa Log in to see NJTEN3
0.30636168 ABIN2680262 FACS ICS PerCP-eFluor® 710 Rat IgG1 kappa Log in to see NJTEN3
0.30636168 ABIN2680264 FACS ICS APC eFluor 780 Rat IgG1 kappa Log in to see NJTEN3
0.30636168 ABIN2680260 FACS ICS PE-Cy7 Rat IgG1 kappa Log in to see NJTEN3
0.30636168 ABIN2680257 FACS ICS PE Rat IgG1 kappa Log in to see NJTEN3
0.0010374015 ABIN964790 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0010374015 ABIN964782 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.0010374015 ABIN2665826 FACS Hamster IgG Log in to see B122 9
0.0010374015 ABIN1102520 FACS IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0010374015 ABIN2665127 FACS Hamster IgG Log in to see B122 9
0.0010374015 ABIN964792 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0010374015 ABIN1102519 FACS IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0010374015 ABIN1832274 FACS PerCP Rat IgG2b Log in to see 11n92
0.0010374015 ABIN1832275 FACS CF Rat IgG2b Log in to see 11n92
0.0010374015 ABIN2661217 FACS Biotin Hamster IgG Log in to see B122 9
0.0010374015 ABIN4324366 ELISA FACS IHC IHC (p) WB Biotin Rabbit Log in to see Polyclonal 1
0.0010374015 ABIN4324365 ELISA FACS ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 3
0.0010374015 ABIN1832271 FACS PE Rat IgG2b Log in to see 11n92
0.0010374015 ABIN2665126 FACS Hamster IgG Log in to see B122 9

Top referenced anti-IL1B antibody for Flow Cytometry

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Application / Reactivity Mouse (Murine)
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ELISA 84 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
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Flow Cytometry (FACS) 23 Antibodies
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Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
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Immunoprecipitation (IP) 14 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine)
(513), (171), (123), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rat
(401), (341), (19), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is conjugated to EFluor 450
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(502), (411), (152), (125), (116), (70), (66), (47), (35), (29), (25), (24), (13), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form.
Characteristics This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form.
Synonyms: Synonyms: Interleukin-1 beta, IL-1F2
Clone NJTEN3
Isotype IgG1 kappa

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-1 beta (IL1B Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Applications reported: This NJTEN3 antibody has been reported for use intracellular staining followed by flow cytometric analysis.

Applications tested: This NJTEN3 antibody has been tested by intracellular staining of stimulated mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 456 nm and is excited with the Violet Page 2 of 2 Anti-Mouse IL-1 beta Pro-form eFluor® 450
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL1B antibody (Interleukin 1, beta)  (EFluor 450) (ABIN2680266) anti-Interleukin 1, beta (IL1B) antibody (EFluor 450)