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anti-Mouse (Murine) IL1B antibody for Blocking Antibody

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Mouse (Murine)
514
171
123
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Rabbit
400
342
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
502
411
153
126
116
70
66
47
36
29
25
24
14
13
12
7
7
4
4
4
4
3
3
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1002570
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00086317933 ABIN289471 Inhibition ELISA WB Rabbit Log in to see Polyclonal

Similar anti-IL1B Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 84 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine)
(514), (171), (123), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(400), (342), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(502), (411), (153), (126), (116), (70), (66), (47), (36), (29), (25), (24), (14), (13), (12), (7), (7), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint mIL-1beta (murine IL-1 beta)
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 1-beta (IL1B Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1beta (murine IL-1 beta). Anti-Murine IL-1beta specific antibody was purified by affinity chromatography employing immobilized mIL-1beta matrix.
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of mIL-1beta (10 pg/µl), a concentration of 0.57 - 0.86 µg/µl of this antibody is required. To detect mIL-1beta by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta. To detect mIL-1beta by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Murine IL-1beta (PK-AB815-61122MB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta. To detect mIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect mIL-1beta by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Murine IL-1beta (PK-AB815-61122MB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta. To detect mIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of mIL-1beta (10 pg/µl), a concentration of 0.57 - 0.86 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months