anti-Mouse (Murine) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
48
34
29
20
16
15
15
14
12
11
11
7
7
7
6
6
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
545
192
128
37
30
20
17
13
12
11
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
409
367
19
19
8
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
96
46
33
26
15
14
12
12
10
10
9
8
8
8
8
8
8
8
4
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
524
415
175
160
112
76
59
54
46
32
31
24
16
14
14
7
6
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN5326748
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 10
3.2182703 ABIN446969 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal 5
3.2182703 ABIN4324366 ELISA FACS IHC IHC (p) WB Biotin Rabbit Log in to see Polyclonal 1
3.2182703 ABIN4324365 ELISA FACS ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 4
3.2182703 ABIN3042621 WB Rabbit IgG AA 250-265, C-Term Log in to see Polyclonal
3.2182703 ABIN3187737 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.2182703 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.2182703 ABIN964790 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN964782 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN4324367 ELISA IHC IHC (p) WB HRP Rabbit Log in to see Polyclonal
3.2182703 ABIN2957636 WB Rabbit Log in to see Polyclonal
3.2182703 ABIN498526 IHC (p) WB Rabbit Log in to see Polyclonal
3.2182703 ABIN4904039 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN3021462 IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN1173157 ICC IHC IP WB Rabbit IgG AA 118-269 Log in to see Polyclonal
3.2182703 ABIN1859383 ICC IHC IP WB Rabbit IgG AA 118-269 Log in to see Polyclonal
3.2182703 ABIN3031437 WB Rabbit IgG C-Term Log in to see Polyclonal
3.2182703 ABIN5531465 FACS IHC (p) WB Rabbit Ig Fraction AA 148-174 Log in to see Polyclonal
3.2182703 ABIN2428273 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN2421713 ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(48), (34), (29), (20), (16), (15), (15), (14), (12), (11), (11), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
(545), (192), (128), (37), (30), (20), (17), (13), (12), (11), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(409), (367), (19), (19), (8), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(96), (46), (33), (26), (15), (14), (12), (12), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(524), (415), (175), (160), (112), (76), (59), (54), (46), (32), (31), (24), (16), (14), (14), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN5326748) Images
Blocking Peptide Blocking peptide for this product available: ABIN978485
Sequence MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL
Predicted Reactivity Dog: 92%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 92%
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B antibody (ABIN5326748) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Alias Symbols: IL-1, IL1-BETA, IL1F2
Protein Interaction Partner: PTPN1, LYN, FYN, CMA1, CASP1, APP, SP1, EGR1, ELAVL1, CASP4, IL1RAP, PRTN3, IL1R2, IL1R1, IL1B, MMP2, ADRB2, A2M, UBE2N, ZNF675, MAPK8IP2,
Protein Size: 269
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B antibody (ABIN5326748) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 269 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B antibody (ABIN5326748) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B antibody (ABIN5326748)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Lee, Lu, Chai, Sheu, Chen, Huang, Kao, Chen, Chung, Sung, Chang, Lee, Yip: "Circulating microparticles enhanced rat vascular wall remodeling following endothelial denudation." in: American journal of translational research, Vol. 8, Issue 11, pp. 4511-4522, 2016

Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-?B p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B antibody (ABIN5326748) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748) Sample Type: Human Macrophange Cells
Green: primary
Red: p...
Western Blotting (WB) image for anti-Interleukin 1, beta (IL1B) (N-Term) antibody (ABIN5326748) WB Suggested Anti-IL1B
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500