anti-Rat (Rattus) IL1B antibody for Functional Studies

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Reactivity
Rat (Rattus)
546
193
127
37
31
20
17
13
13
12
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
409
367
19
19
8
7
6
2
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
96
46
33
26
15
14
12
12
10
10
9
8
8
8
8
8
8
8
4
4
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
524
415
176
160
112
76
59
54
46
31
31
23
16
14
14
7
6
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN115953
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115954 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN2474901 Func ELISA WB Rabbit IgG Log in to see Polyclonal 4
1 ABIN2144076 Func FACS IP Mouse IgG1 Log in to see CRM56
1 ABIN641015 Func ELISA WB Rabbit Log in to see

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Rat (Rattus)
(546), (193), (127), (37), (31), (20), (17), (13), (13), (12), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(409), (367), (19), (19), (8), (7), (6), (2), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(96), (46), (33), (26), (15), (14), (12), (12), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(524), (415), (176), (160), (112), (76), (59), (54), (46), (31), (31), (23), (16), (14), (14), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity Reacts with Interleukin-1-beta.
Cross-Reactivity (Details) Species reactivity (tested):Rat.
Purification Affinity Chromatography using immobilized Rat IL-1 beta matrix.
Immunogen Highly pure (>98%) recombinant Rat IL-1 beta. Remarks: Molecular Weight: ~150 kDa

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background IL1-beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1A and IL1B binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1B is a secreted cytokine, IL-1A is predominantly a cell-associated cytokine.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 10116
UniProt Q63264
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-1 beta (0.20 ng/mL), a concentration of 0.09-0.14 μg/mL of this antibody is required. Sandwich ELISA: To detect Rat IL-1 beta by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with biotinylated Anti-Rat IL-1 beta (PP034B1 or PP034B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant RatIL-1 beta. Western blot: To detect Rat IL-1 beta by Western blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat IL-1 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody is stable at -20 °C for 1 year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Expiry Date 12 months