We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Interleukin 1, beta (IL1B) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This IL1B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-IL1B AntibodyTarget Details IL1B Application Details Handling Images
|Specificity||Reacts with Interleukin-1-beta.|
|Cross-Reactivity (Details)||Species reactivity (tested):Rat.|
|Purification||Affinity Chromatography using immobilized Rat IL-1 beta matrix.|
|Immunogen||Highly pure (>98%) recombinant Rat IL-1 beta. Remarks: Molecular Weight: ~150 kDa|
Target Details IL1BProduct Details anti-IL1B Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)|
|Background||IL1-beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1A and IL1B binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1B is a secreted cytokine, IL-1A is predominantly a cell-associated cytokine.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2|
|Pathways||NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy|
Application DetailsProduct Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-1 beta (0.20 ng/mL), a concentration of 0.09-0.14 μg/mL of this antibody is required. Sandwich ELISA: To detect Rat IL-1 beta by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with biotinylated Anti-Rat IL-1 beta (PP034B1 or PP034B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant RatIL-1 beta. Western blot: To detect Rat IL-1 beta by Western blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat IL-1 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||The lyophilized antibody is stable at -20 °C for 1 year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.|
|Expiry Date||12 months|