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anti-Rat (Rattus) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Reactivity
Mouse (Murine), Rat (Rattus)
533
178
127
39
31
22
18
17
12
11
8
7
7
4
3
2
2
2
1
1
Host
Rabbit
408
351
17
16
8
8
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is conjugated to Biotin
99
48
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
539
433
175
125
109
74
59
58
38
29
25
24
14
14
13
7
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4324366
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0977407 ABIN446969 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal 3
3.0977407 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
3.0977407 ABIN4324365 ELISA FACS ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 3
3.0977407 ABIN3042509 ELISA WB Rabbit IgG AA 117-268 Log in to see Polyclonal
3.0977407 ABIN3185165 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0977407 ABIN3187737 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0977407 ABIN2001591 ELISA WB Rabbit IgG AA 117-268 Log in to see Polyclonal 1
3.0977407 ABIN964790 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN3042621 WB Rabbit IgG AA 250-265, C-Term Log in to see Polyclonal
3.0977407 ABIN3043165 ELISA WB Rabbit IgG AA 249-263, C-Term Log in to see Polyclonal
3.0977407 ABIN3201456 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 1
3.0977407 ABIN964782 FACS IP IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN2957636 WB Rabbit Log in to see Polyclonal
3.0977407 ABIN498526 IHC (p) WB Rabbit Log in to see Polyclonal
3.0977407 ABIN4324367 ELISA IHC IHC (p) WB HRP Rabbit Log in to see Polyclonal
3.0977407 ABIN4904039 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN3021462 IHC WB Rabbit IgG Log in to see Polyclonal
3.0977407 ABIN1173160 ICC IHC IP WB Rabbit IgG AA 117-268 Log in to see Polyclonal
3.0977407 ABIN3031438 ELISA WB Rabbit IgG AA 117-268 Log in to see Polyclonal
3.0977407 ABIN2428273 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 2 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Neutralization (Neut) 15 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 103 Antibodies
Blocking Peptide (BP) 5 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(533), (178), (127), (39), (31), (22), (18), (17), (12), (11), (8), (7), (7), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(408), (351), (17), (16), (8), (8), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is conjugated to Biotin
(99), (48), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(539), (433), (175), (125), (109), (74), (59), (58), (38), (29), (25), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN4324366) Images
Specificity This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
Purification Ion exchange chromatography
Immunogen This antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta produced in E.coli. The MW of recombinant mouse IL-1 beta was 17 kDa.

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN4324366) Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Background Gene Symbol: IL1B
Molecular Weight Theoretical MW: 17 kDa
Gene ID 3553
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN4324366) Images back to top
Application Notes Western Blot 1:2000 - 1:10000, Flow Cytometry 1:10-1:1000, ELISA 1:20000-1:100000, Immunohistochemistry 1:1000-1:5000, Immunohistochemistry-Paraffin 1:10-1:500This antibody recognizes the 17,000 MW mature IL-1Beta . For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells . PBMC are stimulated for 24 hours with 1 % serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta . For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of human or primate IL-1Beta . For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.Flow Cytometry was reported in scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN4324366) Images back to top
Format Lyophilized
Reconstitution Reconstitute with 0.1 mL deionized water.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN4324366)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Fujisaka, Usui, Ikutani, Aminuddin, Takikawa, Tsuneyama, Mahmood, Goda, Nagai, Takatsu, Tobe: "Adipose tissue hypoxia induces inflammatory M1 polarity of macrophages in an HIF-1α-dependent and HIF-1α-independent manner in obese mice." in: Diabetologia, Vol. 56, Issue 6, pp. 1403-12, 2013 (Sample species: Mouse (Murine)). Further details: Flow Cytometry

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN4324366) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta)  (Biotin) (ABIN4324366) Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1Bet...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta)  (Biotin) (ABIN4324366) Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1Bet...