Mouse (Murine) IL1B ELISA Kit

Recommended IL1B Product (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) ELISA Kits
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
79
51
43
19
18
16
12
12
11
10
7
5
4
4
4
2
2
1
Method Type
Sandwich ELISA
Detection Range
81.9-20000 pg/mL
Minimum Detection Limit
81.9 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2506960
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN365172 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see 13
10.090268 ABIN625141 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 5-2000 pg/mL 5 pg/mL Log in to see 41
10.090268 ABIN415642 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see 21
10.090268 ABIN2472097 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
10.090268 ABIN411294 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 12.5-800 pg/mL 12.5 pg/mL Log in to see 9
10.090268 ABIN625142 Sandwich ELISA Tissue Lysate, Cell Lysate Log in to see 8
10.090268 ABIN3198455 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 46.88-3000 pg/mL 46.88 pg/mL Log in to see
10.090268 ABIN924815 Sandwich ELISA Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
10.090268 ABIN1570671 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
10.090268 ABIN1115634 Sandwich ELISA Serum, Plasma, Biological Fluids 7.813-500 pg/mL 7.813 pg/mL Log in to see
10.090268 ABIN1000233 Plasma, Cell Culture Supernatant, Serum, Tissue Homogenate 6.25-400 pg/mL 6.25 pg/mL Log in to see
10.090268 ABIN492075 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.37-1.000 pg/mL 1.37 pg/mL Log in to see 2
10.090268 ABIN1888525 Sandwich ELISA Plasma, Cell Culture Cells 5-2000 pg/mL 5 pg/mL Log in to see
10.090268 ABIN2708531 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 12.5-800 pg/mL 12.5 pg/mL Log in to see
10.090268 ABIN2708532 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
10.090268 ABIN2114190 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
10.090268 ABIN2064443 Cell Culture Supernatant, Serum, Plasma Log in to see
1 ABIN612740 Sandwich ELISA Cell Culture Cells, Plasma, Serum 0.016-1 ng/mL 0.016 ng/mL Log in to see 10
1 ABIN3032810 Competition ELISA Cell Culture Supernatant, Plasma, Serum 2.7-2000 pg/mL 2.7 pg/mL Log in to see
1 ABIN4947734 Sandwich ELISA Log in to see

General

Antigen Interleukin 1, beta (IL1B) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(79), (51), (43), (19), (18), (16), (12), (12), (11), (10), (7), (5), (4), (4), (4), (2), (2), (1)
Method Type Sandwich ELISA
Detection Range 81.9-20000 pg/mL
Minimum Detection Limit 81.9 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL1B ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL-1 beta in mouse serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of mouse IL-1 beta.
Sensitivity 4 pg/mL
Characteristics EasyTest ELISA, a fast ELISA provides a simple and rapid quantitation of antigen in a range of sample matrices. Compared to standard ELISA, EasyTest ELISA requires only one wash, decreasing variations and significantly saves time. The whole process requires less than 2.5 hours and provides customers with high accuracy.
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • mouse IL-1 beta Mix-4x 9 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details IL1B ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL-1 beta (IL1B ELISA Kit Abstract)
Background IL-1α and IL-1β are structurally related polypeptides that share approximately 17 % amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RII has high affinity for IL-1β but functions as a decoy receptor and negative regulator of IL-1β activity. The mouse IL-1β cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1β-converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1β shares 90 % aa sequence identity with cotton rat and rat and 65 %-78 % identity with human.
Molecular Weight 17 kDa
Gene ID 16176
NCBI Accession NP_032387
UniProt P10749
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details IL1B ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 1.5 h
Plate Pre-coated
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for mouse IL-1 beta has been pre-coated onto a microplate. Standards, samples and mouse IL-1 beta mix are pipetted into the wells and any IL-1 beta present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 50 ng/mL standard. Gently vortex to mix.Take 200 μL mouse IL-1 beta standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 20000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. mouse IL-1 beta Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1490 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:2.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of Mouse IL-1 beta Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 1 hour at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details IL1B ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details IL1B ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Interleukin 1, beta (IL1B) ELISA Kit (ABIN2506960) Interleukin 1, beta (IL1B) ELISA Kit