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Human IL1R2 ELISA Kit

Recommended IL1R2 Product (supplied by: Log in to see )

Antigen
Interleukin 1 Receptor, Type II (IL1R2) ELISA Kits
  • CD121b
  • CDw121b
  • IL-1R-2
  • IL-1RT-2
  • IL-1RT2
  • Il1r-2
  • IL1R2c
  • IL1RB
Epitope
AA 14-343
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
11
6
4
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1889297
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN414879 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.30628014 ABIN924851 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.30628014 ABIN1115596 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.30628014 ABIN2708273 Sandwich ELISA Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN1029822 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012155735 ABIN2114250 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012155735 ABIN2684639 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.0012155735 ABIN456451 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN2535312 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012155735 ABIN2948704 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155735 ABIN2691128 Log in to see

Top referenced IL1R2 ELISA Kit for ELISA

Similar IL1R2 ELISA Kits

Application / Reactivity Human
ELISA 12 ELISA Kits

General

Antigen Interleukin 1 Receptor, Type II (IL1R2) ELISA Kits
Epitope AA 14-343
Reactivity Human
Kits with alternative reactivity to:
(11), (6), (4), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details IL1R2 ELISA Kit

Target details Application Details Handling References for IL1R2 Kit (ABIN1889297) Images
Purpose For quantitative detection of human IL1R2 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant human IL1R2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human IL1R2 antibod - 1
  • Lyophilized recombinant human IL1R2 standar - 10ng/tubex2
  • Biotinylated anti- human IL1R2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: F14-E343

Target details

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Antigen
Alternative Name IL1R2 (IL1R2 ELISA Kit Abstract)
Background Interleukin 1 receptor, type II(IL1R2) also known as CD121b(Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta(IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Gene ID 7850
UniProt P27930
Research Area Immunology
Pathways NF-kappaB Signaling

Application Details

Product Details IL1R2 ELISA Kit Target details Handling References for IL1R2 Kit (ABIN1889297) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL1R2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R2 has been precoated onto 96-well plates. Standards(sf21, F14-E343) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL1R2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL1R2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 85, Standard deviation: 3.91, CV(%): 4.6
  • Sample 2: n=16, Mean(pg/ml): 392, Standard deviation: 12.2, CV(%): 3.1
  • Sample 3: n=16, Mean(pg/ml): 750, Standard deviation: 28.5, CV(%): 3.8,
  • Sample 1: n=24, Mean(pg/ml): 96, Standard deviation: 5.95, CV(%): 6.2
  • Sample 2: n=24, Mean(pg/ml): 414, Standard deviation: 22.77, CV(%): 5.5
  • Sample 3: n=24, Mean(pg/ml): 763, Standard deviation: 38.9, CV(%): 5.1
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL1R2 Kit (ABIN1889297)

Product Details IL1R2 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Dale, Nicklin: "Interleukin-1 receptor cluster: gene organization of IL1R2, IL1R1, IL1RL2 (IL-1Rrp2), IL1RL1 (T1/ST2), and IL18R1 (IL-1Rrp) on human chromosome 2q." in: Genomics, Vol. 57, Issue 1, pp. 177-9, 1999

Images

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Supplier Images
ELISA image for IL1R2 ELISA Kit (Interleukin 1 Receptor, Type II) (ABIN1889297) Human IL1R2 PicoKine ELISA Kit standard curve