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|Antigen||Interleukin 1 Receptor Antagonist (IL1RN) Antibodies|
|Conjugate||This IL1RN antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-IL1RN AntibodyTarget Details IL1RN Application Details Handling Images
E.coli derived recombinant Human IL-1RA.
Type of Immunogen: Cell extract
Target Details IL1RNProduct Details anti-IL1RN Antibody Application Details Handling Images back to top
|Alternative Name||IL1RN (IL1RN Antibody Abstract)|
Name/Gene ID: IL1RN
Synonyms: IL1RN, Anakinra, DIRA, IL-1ra, IL-1RN, IL1F3, IL1RA, MVCD4, IL1 inhibitor, IL1RN (IL1F3), IRAP, ICIL-1RA, IL-1ra3
|Pathways||NF-kappaB Signaling, Hormone Transport|
Application DetailsProduct Details anti-IL1RN Antibody Target Details IL1RN Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC-P (5 μg/mL), Neut (2.5 - 3.7 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7435 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-1RA (50 ng/mL) and Human IL-1alpha (50 pg/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required. ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (LS-C104474) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL1RN Antibody Target Details IL1RN Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-IL1RN Antibody Target Details IL1RN Application Details Handling back to top