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Human IL1RN ELISA Kit

Recommended IL1RN Product (supplied by: Log in to see )

Antigen
Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kits
  • DIRA
  • F630041P17Rik
  • ICIL-1RA
  • icIL-1ra
  • IL-1RA
  • IL-1ra
  • IL-1ra3
  • IL-1RN
  • IL1F3
  • IL1RA
  • IL1RN
  • IRAP
  • IRAP1
  • MVCD4
Epitope
AA 26-177
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
26
16
13
10
8
8
7
5
5
5
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN921100
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN414674 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.30628014 ABIN924775 Sandwich ELISA Cell Lysate, Serum, Plasma 23-1500 pg/mL 23 pg/mL Log in to see
0.30628014 ABIN1115590 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.30628014 ABIN2708274 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.30628014 ABIN2114248 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
0.0012155735 ABIN367350 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
0.0012155735 ABIN3032944 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 41.1-30000 pg/mL 41.1 pg/mL Log in to see
0.0012155735 ABIN1028953 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155735 ABIN511406 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012155735 ABIN3198569 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 39.06-2500 pg/mL 39.06 pg/mL Log in to see
0.0012155735 ABIN455515 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012155735 ABIN625020 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 2
0.0012155735 ABIN626502 Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012155735 ABIN625021 Sandwich ELISA Tissue Lysate, Cell Lysate 0.1-30 ng/mL 0.1 ng/mL Log in to see
0.0012155735 ABIN2042533 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN2114247 Cell Lysate, Serum, Plasma 23-1500 pg/mL 23 pg/mL Log in to see
0.0012155735 ABIN2684643 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN2684644 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN1846939 Tissue Homogenate, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN2116108 0.312-20 ng/mL 0.312 ng/mL Log in to see

Top referenced IL1RN ELISA Kit for ELISA

  • Human IL1RN ELISA Kit for Sandwich ELISA - ABIN625020 (2 Publications): Wang, Williams, Krishnan, Liao, Liu, Mao, Shi, Mobley, Woodruff, Cleeland: Serum sTNF-R1, IL-6, and the development of fatigue in patients with gastrointestinal cancer undergoing chemoradiation therapy. in Brain, behavior, and immunity 2012 (PubMed)

  • Human IL1RN ELISA Kit for Sandwich ELISA - ABIN921100 (1 Publications): Patterson, Jones, Hart, Bleskan, Berger, Geyer, Eisenberg, Smith, Arend: The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region. in Genomics 1993 (PubMed)

Similar IL1RN ELISA Kits

Application / Reactivity Human
ELISA 26 ELISA Kits

General

Antigen Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kits
Epitope AA 26-177
Reactivity Human
Kits with alternative reactivity to:
(26), (16), (13), (10), (8), (8), (7), (5), (5), (5), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details IL1RN ELISA Kit

Target details Application Details Handling References for IL1RN Kit (ABIN921100) Images
Purpose For quantitative detection of human IL-1RA in cell culture supernates, serum and plasma( heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-1RA
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human IL-1RA antibod - 1
  • Lyophilized recombinant human IL-1RA standar - 10ng/tubex2
  • Biotinylated anti- human IL-1RA antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: R26-E177

Target details

Product Details IL1RN ELISA Kit Application Details Handling References for IL1RN Kit (ABIN921100) Images back to top
Antigen
Alternative Name IL1RN (IL1RN ELISA Kit Abstract)
Background The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
Gene ID 3557
UniProt P18510
Pathways NF-kappaB Signaling

Application Details

Product Details IL1RN ELISA Kit Target details Handling References for IL1RN Kit (ABIN921100) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-1RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-1RA standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 80, Standard deviation: 4.64, CV(%): 5.8
  • Sample 2: n=16, Mean(pg/ml): 574, Standard deviation: 25.83, CV(%): 4.5
  • Sample 3: n=16, Mean(pg/ml): 1395, Standard deviation: 61.38, CV(%): 4.4,
  • Sample 1: n=24, Mean(pg/ml): 76, Standard deviation: 7, CV(%): 9.2
  • Sample 2: n=24, Mean(pg/ml): 509, Standard deviation: 37.16, CV(%): 7.3
  • Sample 3: n=24, Mean(pg/ml): 1213, Standard deviation: 77.6, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details IL1RN ELISA Kit Target details Application Details References for IL1RN Kit (ABIN921100) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL1RN Kit (ABIN921100)

Product Details IL1RN ELISA Kit Target details Application Details Handling Images back to top
Background publications

Patterson, Jones, Hart, Bleskan, Berger, Geyer, Eisenberg, Smith, Arend: "The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region." in: Genomics, Vol. 15, Issue 1, pp. 173-6, 1993

Images

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Supplier Images
ELISA image for IL1RN ELISA Kit (Interleukin 1 Receptor Antagonist) (ABIN921100) Human IL-1RA PicoKine ELISA Kit standard curve