Human IL1RN ELISA Kit

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Antigen
Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kits
  • IL1RN
  • DIRA
  • ICIL-1RA
  • IL-1RN
  • IL-1ra
  • IL-1ra3
  • IL1F3
  • IL1RA
  • IRAP
  • MVCD4
  • IRAP1
  • F630041P17Rik
  • icIL-1ra
  • IL-1RA
  • interleukin 1 receptor antagonist
  • interleukin 1 receptor, type I
  • IL1RN
  • Il1rn
  • IL1R1
Epitope
AA 26-177
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
27
16
14
11
9
7
7
7
4
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Options
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Catalog No. ABIN921100
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.499232 ABIN625020 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 0.1-30 ng/mL 0.1 ng/mL Log in to see 3
12.499232 ABIN1028953 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.499232 ABIN367350 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
12.499232 ABIN414674 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.499232 ABIN3198569 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 39.06-2500 pg/mL 39.06 pg/mL Log in to see
12.499232 ABIN924775 Sandwich ELISA Cell Lysate, Serum, Plasma 23-1500 pg/mL 23 pg/mL Log in to see
12.499232 ABIN1115590 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.499232 ABIN625021 Sandwich ELISA Tissue Lysate, Cell Lysate 0.1-30 ng/mL 0.1 ng/mL Log in to see
12.499232 ABIN2708274 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
12.499232 ABIN2114248 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin) Log in to see
12.499232 ABIN5564674 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
1 ABIN3032944 Competition ELISA Cell Culture Supernatant, Plasma, Serum 41.1-30000 pg/mL 41.1 pg/mL Log in to see
1 ABIN511406 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN5592204 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN455515 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5520863 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN2114247 Cell Lysate, Serum, Plasma 23-1500 pg/mL 23 pg/mL Log in to see
1 ABIN2042533 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2684643 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN2684644 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 31.2-2000 pg/mL 31.2 pg/mL Log in to see

General

Antigen Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kits
Epitope AA 26-177
Reactivity Human
Kits with alternative reactivity to:
(27), (16), (14), (11), (9), (7), (7), (7), (4), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details IL1RN ELISA Kit

Target details Application Details Handling ProductDetails: References for IL1RN Kit (ABIN921100) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1RA
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: E.coli
Immunogen sequence: R26-E177
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: R26-E177

Target details

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Antigen
Alternative Name IL1RN (IL1RN ELISA Kit Abstract)
Background

Protein Function: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2, however, the physiological relevance of the latter association is unsure. .

Background: The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Synonyms: Interleukin-1 receptor antagonist protein,IL-1RN,IL-1ra,IRAP,ICIL-1RA,IL1 inhibitor,Anakinra,IL1RN,IL1F3, IL1RA,

Full Gene Name: Interleukin-1 receptor antagonist protein

Cellular Localisation: Isoform 1: Secreted.
Gene ID 3557
UniProt P18510
Pathways NF-kappaB Signaling, Hormone Transport

Application Details

Product Details IL1RN ELISA Kit Target details Handling ProductDetails: References for IL1RN Kit (ABIN921100) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Tissue Specificity: The intracellular form of IL1RN is predominantly expressed in epithelial cells.

Plate Pre-coated
Protocol human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-1RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-1RA standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 80, Standard deviation: 4.64, CV(%): 5.8
  • Sample 2: n=16, Mean(pg/ml): 574, Standard deviation: 25.83, CV(%): 4.5
  • Sample 3: n=16, Mean(pg/ml): 1395, Standard deviation: 61.38, CV(%): 4.4,
  • Sample 1: n=24, Mean(pg/ml): 76, Standard deviation: 7, CV(%): 9.2
  • Sample 2: n=24, Mean(pg/ml): 509, Standard deviation: 37.16, CV(%): 7.3
  • Sample 3: n=24, Mean(pg/ml): 1213, Standard deviation: 77.6, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details IL1RN ELISA Kit Target details Application Details ProductDetails: References for IL1RN Kit (ABIN921100) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for IL1RN Kit (ABIN921100)

Product Details IL1RN ELISA Kit Target details Application Details Handling Images back to top
Background publications

Patterson, Jones, Hart, Bleskan, Berger, Geyer, Eisenberg, Smith, Arend: "The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region." in: Genomics, Vol. 15, Issue 1, pp. 173-6, 1993

Images

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Supplier Images
ELISA image for Interleukin 1 Receptor Antagonist (IL1RN) ELISA Kit (ABIN921100) Human IL-1RA PicoKine ELISA Kit standard curve