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|Application / Reactivity||Mouse (Murine)||Human||Cow (Bovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||5 Antibodies||4 Antibodies|
|Cytometry by Time of Flight (CyTOF)||14 Antibodies||10 Antibodies||8 Antibodies|
|Western Blotting (WB)||188 Antibodies||87 Antibodies||95 Antibodies|
|Radioimmunoassay (RIA)||32 Antibodies||18 Antibodies||3 Antibodies|
|Protein Assay (PrA)||6 Antibodies||2 Antibodies|
|Neutralization (Neut)||2 Antibodies||2 Antibodies|
|Immunoradiometric Assay (IRMA)||1 Antibodies|
|Immunoprecipitation (IP)||65 Antibodies||15 Antibodies|
|Antigen||Insulin (INS) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine) Alternatives|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN4900790) Images
|Specificity||Detects bovine, human, and mouse insulin.|
|Purification||Protein A or G purified from hybridoma culture supernatant|
|Sterility||0.2 μm filtered|
|Immunogen||E. coli-derived recombinant human Insulin|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN4900790) Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||Alternative Names: IDDM2, ILPR, INS, insulin, IRDN, MODY10, proinsulin|
|Pathways||NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN4900790) Images back to top
Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN4900790) Images back to top
|Reconstitution||Reconstitute at 0.5 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-Insulin Antibody (ABIN4900790)Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
|Product cited in:||
Cucak, Grunnet, Rosendahl: "Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization." in: Journal of leukocyte biology, Vol. 95, Issue 1, pp. 149-60, 2014 Method employed by authors: Flow Cytometry (FACS) (Sample species: Mouse (Murine)).
Kalis, Bolmeson, Esguerra, Gupta, Edlund, Tormo-Badia, Speidel, Holmberg, Mayans, Khoo, Wendt, Eliasson, Cilio: "Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus." in: PLoS ONE, Vol. 6, Issue 12, pp. e29166, 2012 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).
Hagberg, Mehlem, Falkevall, Muhl, Fam, Ortsäter, Scotney, Nyqvist, Samén, Lu, Stone-Elander, Proietto, Andrikopoulos, Sjöholm, Nash, Eriksson: "Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes." in: Nature, Vol. 490, Issue 7420, pp. 426-30, 2012 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).
Jin, Li, Xu, Geng, He, Su, Tao, Ding, Wang, Hu: "Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1." in: Journal of cellular biochemistry, Vol. 104, Issue 1, pp. 224-36, 2008 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).
Dowling, ODriscoll, OSullivan, Dowd, Henry, Jeppesen, Meleady, Clynes: "Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress." in: Proteomics, Vol. 6, Issue 24, pp. 6578-87, 2006 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).
ImagesProduct Details anti-Insulin Antibody Target Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN4900790) back to top