anti-Cow (Bovine) Insulin antibody for Western Blotting

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Cow (Bovine), Human, Pig (Porcine)
965
396
302
282
262
121
49
6
6
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
842
183
54
25
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is un-conjugated
57
42
41
28
28
27
23
23
18
17
17
17
17
17
17
16
16
12
11
11
11
11
11
5
5
5
5
5
5
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
622
372
248
233
208
204
161
142
71
66
40
27
18
11
9
8
8
7
5
4
3
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN487416
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1469584 ABIN411565 IHC ELISA WB Mouse IgG1 full length Log in to see HPI-B5
3.1469584 ABIN1172907 ICC IHC IP WB Rabbit IgG AA 25-54 Log in to see Polyclonal
1 ABIN272321 EIA IHC (p) WB Mouse IgG1 Log in to see E2E3 3
1 ABIN4326102 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM139
1 ABIN4347575 ELISA IHC (p) WB Mouse IgG1 Log in to see HPI-B5
1 ABIN4326055 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326056 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326054 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326053 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326057 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326058 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG Log in to see E2-E3-2D11-H5 (same as INS04-INS05)
1 ABIN4326069 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326072 ELISA ICC IF IHC (fro) IHC (p) WB Biotin Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326073 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326074 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326080 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326082 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326068 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326070 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)
1 ABIN4326081 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 kappa Log in to see E2-E3 (same as INS04)

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Pig (Porcine)
(965), (396), (302), (282), (262), (121), (49), (6), (6), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(842), (183), (54), (25), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is un-conjugated
(57), (42), (41), (28), (28), (27), (23), (23), (18), (17), (17), (17), (17), (17), (17), (16), (16), (12), (11), (11), (11), (11), (11), (5), (5), (5), (5), (5), (5), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(622), (372), (248), (233), (208), (204), (161), (142), (71), (66), (40), (27), (18), (11), (9), (8), (8), (7), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Specificity The antibody recognizes Insulin and recombinant Proinsulin, but does not react with synthetic C-Peptide.
Characteristics Synonyms: INS
Purification Affinity Chromatography on Protein G.
Immunogen Recombinant full length Insulin expressed in Yeast.
Clone HPI-B5
Isotype IgG1

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background Proinsulin is a prohormone precursor to Insulin, made by the pancreas in response to increased blood sugar. Proinsulin is 39 amino acids longer than the mature Insulin, of which 4 are completely lost and the remaining 35 forms the C-peptide. The measurement of Proinsulin in serum provides a useful, valuable information for the diagnosis of insulinomas. Proinsulin levels have also been shown to be elevated in non-insulin dependent diabetics (NIDDM), in insulin dependent diabetics (IDDM) and other clinical situations.Synonyms: INS
Gene ID 3630
UniProt P01308
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Indirect ELISA: Use at 2 μg/mL (For detection of antigen). Western Blot: Use at 4-5 μg/mL. Immunohistochemistry: Use at 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS containing containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °CAvoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Insulin (INS) antibody (ABIN487416) anti-Insulin (INS) antibody
Western Blotting (WB) image for anti-Insulin (INS) antibody (ABIN487416) anti-Insulin (INS) antibody (Image 2)