|Application / Reactivity||Human||Pig (Porcine)||Rat (Rattus)||Hamster|
|Western Blotting (WB)||102 Antibodies||19 Antibodies||22 Antibodies|
|Radioimmunoassay (RIA)||37 Antibodies||14 Antibodies||4 Antibodies|
|Radial Immunodiffusion (RID)||1 Antibodies|
|Protein Assay (PrA)||6 Antibodies||2 Antibodies|
|Immunoradiometric Assay (IRMA)||1 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies||1 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraformaldehyde) (IHC (pfa))||1 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||118 Antibodies||56 Antibodies||37 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||167 Antibodies|
|Antigen||Insulin (INS) Antibodies|
|Reactivity||Hamster, Human, Pig (Porcine), Rat (Rattus) Alternatives|
|Host||Guinea Pig Alternatives|
|Conjugate||This Insulin antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|4 references available|
|Supplier||Log in to see|
Product Details anti-Insulin AntibodyTarget Details Insulin Application Details Handling References for anti-Insulin Antibody (ABIN115757) Images
|Specificity||The antibody recognizes Insulin produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors. Absorption with 10-100 μg proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and Insulin only partly reduce staining.|
|Cross-Reactivity (Details)||Also reacts to: Proinsulin|
|Immunogen||Synthetic Human proinsulin|
Target Details InsulinProduct Details anti-Insulin Antibody Application Details Handling References for anti-Insulin Antibody (ABIN115757) Images back to top
|Alternative Name||Insulin (INS Antibody Abstract)|
|Background||Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.Synonyms: INS|
|Research Area||Neurology, Diabetes, Hormones|
|Pathways||NF-kappaB Signaling, RTK Signaling|
Application DetailsProduct Details anti-Insulin Antibody Target Details Insulin Handling References for anti-Insulin Antibody (ABIN115757) Images back to top
Immunofluorescence: 1: 1000 - 1: 2000, overnight incubation at 2-8C. Immunohistochemistry on frozen and paraffin sections: 1: 3000 - 1: 5000. Positive Control: Formalin-fixed paraffin sections of human pancreas
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Insulin Antibody Target Details Insulin Application Details References for anti-Insulin Antibody (ABIN115757) Images back to top
|Reconstitution||Dissolve the antiserum in 50-100 μL distilled water, and dilute further in 0.1 M PBS with 1 % BSA and 0.09 % Sodium Azide|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.|
References for anti-Insulin Antibody (ABIN115757)Product Details anti-Insulin Antibody Target Details Insulin Application Details Handling Images back to top
Kohnert, Axcrona, Hehmke et al.: "Islet neuronal abnormalities associated with impaired insulin secretion in type 2 diabetes in the Chinese hamster." in: Regulatory peptides, Vol. 82, Issue 1-3, pp. 71-9, 1999 (PubMed).
Ahrén, Gudbjartsson, Al-Amin et al.: "Islet perturbations in rats fed a high-fat diet." in: Pancreas, Vol. 18, Issue 1, pp. 75-83, 1999 (PubMed).
Myrsén-Axcrona, Karlsson, Sundler et al.: "Dexamethasone induces neuropeptide Y (NPY) expression and impairs insulin release in the insulin-producing cell line RINm5F. Release of NPY and insulin through different pathways." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10790-6, 1997 (PubMed).
Myrsén, Ahrén, Sundler: "Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin." in: Diabetes, Vol. 45, Issue 10, pp. 1306-16, 1996 (PubMed).